The cryopreservation process adversely affects sperm function and quality traits, causing some changes at biochemical and structural levels, due to mechanical, thermal, osmotic, and oxidative damage. Supplementation with curcumin nanoparticles could prevent and even revert this effect and could enhance the post/thawed sperm quality in the rabbit. The study amid to explore the effect of curcumin (CU) and curcumin nanoparticles (CUNPs) supplementation in semen extender on post/thawed rabbit sperm quality. Twelve fertile, healthy rabbit bucks were included, and the ejaculates were collected using artificial vaginas. Rabbit pooled semen was cryopreserved in tris-yolk fructose (TYF) extender without any supplement (control group) or extender supplemented with CU at levels of 0.5, 1 or 1.5 µg/mL (CU0.5, CU1.0, and CU1.5, respectively) or CUNPs at levels of 0.5, 1, 1.5 (CUNPs0.5, CUNPs1.0, and CUNPs1.5, respectively) and was packed in straws (0.25 mL) and stored in liquid nitrogen (−196 °C). Results revealed that CUNPs1.5 had a positive influence (p < 0.05) on post-thawing sperm progressive motility, viability, and membrane integrity as compared with the other groups. Percentages of dead sperm, abnormalities, early apoptotic, apoptotic, and necrotic sperm cells reduced (p < 0.05) in CUNPs1.5 as compared to other treatments. Using 1.5 µg/mL of CUNPs significantly improved total antioxidant capacity (TAC), GPx, while MDA and POC reduced (p < 0.05) in CU1.5 in comparison with other groups. SOD values were enhanced (p < 0.05) in CUNPs1.0 and CUNPs1.5 in relation with other treatments. Conclusively, the addition of curcumin and its nanoparticles to the extender can improve the post-thawed quality of rabbit sperm via redox signaling and reduce the apoptosis process.

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This study explored the influence of triclosan (TCS) in the absence and presence of sodium fluoride (NaF) on estrogenic activity and thyroid function of adolescent female rats. The results indicated that the individual exposure to TCS evoked a significant decline in T and T but the levels of estradiol, FSH, and LH were significantly elevated beside marked up regulation of calbindin-D9k and estrogen α mRNA expression. On the other hand, the single exposure to NaF causes insignificant changes in thyroid hormones, but evoked a trend toward an increase in both estradiol and LH levels. No significant differences in the TSH level were recorded among the experimental groups. The joint exposure to TCS and NaF induced a significant improvement in thyroid and reproductive hormone levels. Overall, these findings revealed that exposure to TCS resulted in significant endocrine and reproductive alterations in immature female rats, while TCS + NaF coexposure resulted in lessening most effects.

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Introduction Hexavalent chromium Cr (VI) is a strong oxidizing toxic agent. It penetrates cell membrane and quickly reduced with production of reactive intermediates and reactive oxygen species that react with the DNA causing anomalies in the cell structure. Vitamin E (vit. E) is a lipid soluble antioxidant preventing damage to membranes also selenium (Se) is an essential micronutrient with an antioxidant activity. Aim of the present study was to evaluate the hepatotoxicity, nephrotoxicity, and genotoxicity induced by acute Cr (VI) toxicity. Also, to evaluate and compare the possible protective role of vit. E and Se against that toxicity. Methodology: This study was carried out on 60 adult male albino rats divided into 10 rats of six groups, negative control group, selenium control group (0.5 mg/kg IP for 5 consecutive days), vitamin E control group (125 mg/kg orally for 14 days), Cr (VI) group (10 mg/kg single dose IP), Cr (VI) + Selenium group and hexavalent chromium + Vitamin E group. Liver and kidney function tests, total protein, oxidative stress, antioxidant markers and genotoxic analysis were done to all groups. Result: Acute Cr(VI) toxicity resulted in increased levels of the studied liver, kidney , oxidative stress markers, all forms of chromosomal aberrations and elevation of DNA damage. It decreased levels of total protein and antioxidant markers. Treatment with Se or vit. E resulted in improvement in all these effects. Conclusion& recommendation: Cr (VI) is a hepatotoxic, nephrotoxic, and genotoxic. Se or vit. E has the ability for reduction of these deleterious effects. So it is recommended to do regular medical examination of workers exposed to hexavalent chromium for early detection of any health problem and afford dietary supplementation with vitamin E and selenium.