Four plant species belonging to the family Lamiaceae were assessed with different molecular markers using five RAPD, six inter simple sequence repeats (ISSR), and six start codon targeted (SCoT) primers to detect their levels of genetic diversity. The RAPD primers identified a total of 41 amplified bands, while all primers inducing five unique markers among the four species used in this study, furthermore the polymorphism percentage reached to 73.71%. ISSR primers generated 31 amplified bands including eight unique markers with a polymorphism percentage reached to 54.83%. SCoT primers exhibited a total of 43 amplified bands. Four of these primers revealed 14 unique genotype specific markers with a polymorphism percentage reached to 39.53%. The four Lamiaceae species were separated into two major groups using cluster analysis: the first group comprised Phlomis floccosa and Salvia officinals, while the second group included Teucrium polium and Thymus capitatus.
Background: The use of ultrasonography as a complementary diagnostic tool to CT in the assessment of cancer larynx has always been underestimated due to the anatomical obstacles in the form of thyroid cartilage calcification yet specific anatomic sites with proper technique implementation raised the sensitivity and specificity of the sonographic results and presented it as a trustable screening tool taking advantage of its additional dynamic criteria. Aim of work: To evaluate the usefulness of ultrasonography versus Computed Tomography in assessing cancer larynx. Patients and Methods: 46 patients with histopathologically proven laryngeal carcinoma were enrolled after exclusion of 5 patients from surgery. A comparative assessment was made between the detection rate of localization and sensitivity, specificity, PPV, NPV and accuracy of pre-therapeutic ultrasonography compared to CT with post operative pathology as a standard reference. Results: Most of the patents were males [47(92.2%)/51] and aged > 60 years [25(49%)/51]. The detection rate was highest in glottic lesions being the same in both ultrasonography and CT [32 (96.9%)/33] with a sensitivity and specificity of 90.9% and 84.6% versus 93.9% and 92.3% in each modality respectively. (p=0.000). The specificity, sensitivity, PPV, NPV and accuracy results of invasion of the intra-laryngeal structures were comparable for both modalities:
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