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The current study was carried out to evaluate the nutritive value of mushroom-uncultivated and -cultivated substrates, and their in vitro gas and methane production. The experiment was conducted in a completely randomized design, and analyzed with GLM using SAS 9.4. Analysis of the structural morphology of mushroom-cultivated substrate was performed using a scanning electron microscope. Mushroom cultivation led to lower ether extract, acid detergent fiber, and crude fiber level of substrate (p < 0.05). Mushroom-cultivated substrate showed higher in vitro cumulative gas production (p < 0.05). Moreover, mushroom cultivation led to a higher sample surface, and improved the microorganisms’ access to feed materials, thus stimulating rumen fermentation and increasing methane production (p < 0.05). The organic matter digestibility, metabolizable energy, and net energy lactation values were higher for mushroom-cultivated substrate than uncultivated substrate. The results demonstrate that mushroom-cultivation not only increases the contact surface of cellulose, leading to higher ruminal microorganisms’ access to feedstuff, but could also had higher nutritive value; this material might be used in ruminant ration formulation, to reduce environmental pollution and feed costs.

This study was carried out to determine the effects of different doses (10, 20 and 30%) of sumac shrub leaf substitution instead of corn silage in sheep rations on in vitro gas and methane production, metabolic energy (ME), net energy lactation (NEL) and organic matter digestion degree. Sheep ration consisting of corn silage (20%), alfalfa straw (22.5%), dry meadow grass (20%), and commercial feed (37.5%) constituted the control group. The experimental groups were formed by substituting 10 (S1), 20 (S2) and 30 (S3) percent sumac shrub leaves for corn silage in the control (C) group formed the experimental groups. The effect of sumac shrub leaf substitution on in vitro gas and methane production, metabolic energy, net energy lactation, and organic matter digestion degree was found to be significant. The 24-hour in vitro gas production values of rations ranged between 43.11- 46.77 ml/200 mg DM, methane production values 6.8-7.48 ml, metabolic energy values 8.91-9.41 MJ/kg DM, net energy lactation, 5.59-5.95 MJ/kg DM and organic matter digestion degree values found between 64.25 and 67.61%. As a result, it was determined that increasing doses of sumac shrub leaf substitute reduced gas and methane production. In addition, it was concluded that the data obtained should be supported by determining the microorganism counts, feed consumption amounts, and feed efficiency coefficients with in vivo studies.

The purpose of this study was to determine the chemical content, in vitro gas and methane production, relative feed value (RFV), relative feed quality (RFQ), in vitro digestibility parameters, and microbial protein production of the Italian ryegrass plants harvested in different periods of the 2020 production season in Erzincan. In this study an ANKOM Daisy Incubator was used to determine the in vitro digestibility parameters. In vitro gas production technique was used todetermine gas production and predicted parameters. It was found that there were significant differences between the Italian ryegrass plants harvested in different periods in terms of composition, RFV, RFQ, and in vitro digestibility and fermentation parameters. The RFV, metabolizable energy (ME), net energy (NE), true NDF digestibility (TNDFD), organic matter digestibility degree (OMDD), total digestible nutrient (TDN), dry matter intake (DMI), and RFQ values of the Italian ryegrass plants were found to be 247.32, 9.13, 5.68, 62.26%, 54.15%, 55.35%, 4.82%, and 215.81, respectively. On the other hand, after 24 hours of incubation it’s in vitro gas (GP), and methane production (ml and %), true dry matter digestibility (TDMD) values, partitioning factor (PF), microbial protein (MP), microbial protein synthesis efficiency (MPSE), and true digestibility (TD) were found to be 105.41, 17.35, 16.42, 281.72, 2.68, 57.68, 20.32, and 59.82, respectively. In conclusion, determining the digestibility of plants via the measurement of RFV, RFQ, digestion parameters, and gas production in different harvest periods provided insights into the potential of Italian ryegrass as a feed material.