ÖZETAmaç: Bu çalışmanın amacı iki farklı self-etch adeziv sistemin [Clearfil SE Bond (CSE) ve G-Bond (GB)] biyouyumluluk ve dentine bağlanma dayanımlarının değerlendirilmesidir. Gereç -Yöntem: Çalışma için 8 adet çekilmiş çürük-süz insan üçüncü azı dişi kullanıldı. Tüm dişlerin oklüzal yüzeyleri düz bir dentin yüzeyi elde etmek için dişin uzun eksenine dik olacak şekilde aşındırıldı. Dişler rastgele 2 gruba ayrıldı ve dişlerin oklüzal yüzeylerine adeziv sistemler üretici firmanın önerileri doğrultu-sunda uygulandıktan sonra Gradia Direct kompozit ile restore edildi. Örnekler, bağlantı ara yüzeyine dik olacak şekilde kesildi (bağlantı alanı: 1 mm 2 ) ve her grup için 15 çubuk elde edildi. Çubuklar daha sonra mikrogerilim bağlanma testine (Microtensile tester, Bisco, Schaumburg, IL, ABD) tabi tutuldu. Veriler tek yönlü varyans analizi kullanılarak analiz edildi. Biyouyumluluğun değerlendirilmesi için agar overlay testi yapıldı. 24 saat sonra inverted mikroskopla hücre dekolarizasyon zonu ve hücre lizis skorları tespit edildi. Veriler Kruskal wallis ve Mann withney U testi ile değerlendirildi. Bulgular: CSE (23.5 MPa), GB (18.9 MPa) dan daha yüksek bağlanma dayanımı gösterdi (p<0.05). Agar overlay testi sonuçlarına göre ise her iki adeziv de pozitif kontrol grubundan daha az hücre lizisi ve dekolarizasyon zonu gösterdi (p<0.001). Sonuç: Kullanılan bonding sistemlerin sitotoksisiteleri arasında istatistiksel olarak önemli bir fark olmadığı için daha yüksek bağlanma dayanımına sahip olan adeziv sistem (CSE) klinik olarak tercih edilebilir. Anahtar Kelimeler: Biyouyumlu materyaller, dentin yapıştırma ajanları, sitotoksisite testleri. ABSTRACT Objectives:The aim of this study was to evaluate the biocompatibility and dentin bond strengths of two different self-etch adhesive systems[Clearfil SE Bond (CSE) and G-Bond (GB)]. Materials and methods: Non-carious 8 extracted human third molar teeth were used in this study. The occlusal surfaces of all teeth were ground perpendicular to the long axis of each tooth to expose a flat dentin surface. The teeth were divided into two groups and adhesive systems were applied according to the manufacturer's instructions to the oclusal surfaces of the teeth and restored with Gradia Direct composite resin. The samples were sectioned perpendicular to the adhesive interface to produce sticks (adhesive area: 1 mm 2 ) and fifteen sticks were obtained for each group. All sticks submitted to microtensile bond strength test (Microtensile tester, Bisco, Schaumburg, IL, ABD). Data were analyzed using one-way varyans analysis ANOVA. An agar overlay test was used for the cytotoxicity evaluation. Cytotoxicity was examined by measuring the zones of cell decolorization and evaluating cell lysis by an inverted microscope after 24 hours. Data were analyzed using Kruskal wallis and Mann withney U test. Results: CSE showed higher dentin bond strength mean (23.5 MPa) than GB (18.9 MPa) (p < 0.05). Both bonding systems showed lower lysis scores than positive control material (p<0.001). Conclusions:The bondi...
The present in vitro study showed that water rinsing is necessary if cured adhesive resin is contaminated with saliva to ensure adequate bond strength.
Abstract. Purpose: This in vitro study evaluated the microleakage of glass carbomer (Glass Fill, GCP Dental, Vianen, Netherlands), resin-modified glass ionomer (Fuji II LC, GC, Japan) and selfadhering flowable composite (Vertise Flow, Kerr, USA) materials. Materials And Methods: Class V cavities were prepared in the occlusal margin of enamel and gingival margin of dentin on both buccal and lingual surfaces of 45 human molar teeth and restored with self-adhering materials according to manufacturers' directions (n=15). The specimens were immersed in 2% basic fuchsine dye at 37ºC for 24 hours. The teeth were sectioned into two pieces buccolingually in an occlusoapical direction and evaluated for microleakage using a stereo microscope (30×) and the degree of microleakage was evaluated using specific scoring criteria. The data were analyzed using KruskalWallis, Mann-Whitney U, and Wilcoxon signed-rank tests. Results: When the self-adhering materials were compared, Glass Fill showed the highest leakage scores but was statistically different from only Vertise flow in the gingival surfaces (p<0.05). In the occlusal surfaces all tested selfadhering materials exhibited similar degrees of microleakage at the enamel margins (p>0.05). Conclusion:Glass Carbomer based self-adhering material showed more microleakage than resin based self adhering materials in the gingival surfaces, but in the occlusal surfaces all of the tested materials showed good performance.
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