The standard therapy for rectal carcinoma is surgical, however, preoperative radiochemotherapy will play an increasing role especially in locally advanced disease. To estimate the prognosis and the effect of radiochemotherapy the postradiochemotherapeutical pathological features are important to assess. We examined the surgical specimens of 17 patients after preoperative radiochemotherapy to estimate and grade the histological reactions. A proposal for a grading system for tumor regression (not yet available in the literature) has also been described. All but one of the carcinomas showed different degrees of tumor regression. A total regression was not observed after standardised pathological work up. In only one case a locally curative resection was not possible. We think that preoperative radiochemotherapy is able to reduce tumor mass thus achieving operability in non-curatively operable cases. We recommend standards of pathological work up and regression grading for further studies comparing surgery and radiochemotherapy of rectal carcinoma.

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now widely used for marker/multi-biomarker detection in medical diagnosis. We tested a new protocol for bacterial identification from blood culture broths in hospital routine by using collection tubes with separator gels on 503 included samples examined over 3 months, where 1.5 mL was injected by a syringe into BD Vacutainer tubes from BACTEC-positive bottles, before processing for bacterial protein extraction. Samples were loaded in duplicate onto the MALDI MS target, allowing a series of 12 samples to be processed in duplicate within 80 min by using Biflex III and BioTyper 2.0 software (Bruker). Including polymicrobial samples, 193 of 213 of Gram-negative bacteria (91.08%) and 284 of 319 of Gram-positive bacteria (89.02%) were correctly identified at the species level. Enterobacteriaceae constituted 35.15% of all species found, Staphylococaceae 37.96%, Streptococaceae and Enterococaceae 20.85%, Pseudomonadaceae 1.69%, and anaerobes 2.44%. In most of the polymicrobial samples, one of the species present was identified (80.9%). Seven isolates remained misidentified as Streptococcus pneumoniae, all belonging to Streptococcus mitis. Staphylococcus aureus was identified better when grown on anaero-aerobic medium, and MALDI BioTyper identification scores as low as 1.4 were pertinent, provided that four successive proposals of the same species were given. This new protocol correlates with conventional microbiology procedures by up to 90%, and by >95% for only monomicrobial samples, and provides a decreased turn-around time for identification of bacteria isolated from blood cultures, making this technology suitable also for blood cultures, with less delay and cost decreases in bacterial diagnostics, and favouring better care of patients.

Purpose The purpose of this study was to analyse patient and injury characteristics as well as arthroscopic findings in a prospective cohort of ACL‐injured patients with or without an associated ramp lesion. Methods Two hundred and twenty‐four patients undergoing a primary (n = 196) or revision (n = 28) ACL reconstruction were included. The presence of a ramp lesion was determined by a systematic arthroscopic inspection of the posteromedial compartment. Chi‐square tests were used to compare the population of ACL‐injured patients with and without a ramp lesion regarding sex, age, body mass index, previous ACL injuries, sport before injury, and injury characteristics. Significance was set at p < 0.05. Results Fifty‐three out of 224 patients had a ramp lesion (24%). The presence of the latter was not related to any of the analysed patient characteristics. The prevalence of the lesion was higher in contact injuries (n = 19; 41%) compared with non‐contact injures (n = 34; 19%; p < 0.001). It was higher in patients with complete ACL ruptures (n = 49; 27%) as opposed to partial ruptures (n = 1; 4%; p = 0.01). A patient was 2.98 [95% CI 1.49–5.98] times more likely to have a ramp lesion if the ACL injury was declared to have been caused by direct contact and 8.71 [95% CI 1.15–66.12] times more likely if the ACL tear was complete. Conclusion Ramp lesions may be anticipated in almost one out of four patients undergoing ACL reconstruction, especially if a patient sustained a contact injury and in the presence of a complete ACL tear. Level of evidence III.

No abstract

Purpose The purpose of this study was to compare preoperative knee laxity between two groups of patients with primary or revision ACL reconstruction with or without an associated ramp lesion of the medial meniscus. Methods Two‐hundred and seventy‐five patients with an ACL reconstruction (243 primaries; 32 revisions) were prospectively screened using direct arthroscopic visualisation and divided into a ramp lesion group (RLG) and a control group (CG) regardless of the presence of other associated meniscal tears. All patients were clinically examined under anaesthesia before surgery by grading the Lachman and pivot shift tests. Results Fifty‐eight patients were included in the RLG. The CG included 217 patients. With all meniscus lesions included, there were no significant differences between the two groups. After excluding all other meniscus lesions in both groups except for ramp lesions in the RLG, the prevalence of a grade III pivot shift was higher in the RLG (32 remaining patients; 47% grade III) compared to the CG (91 remaining patients; 24% grade III, p = 0.02). The difference of patients with a grade III pivot shift between the CG and RLG remained significant after removal of revision ACL reconstructions (CG, 85 remaining patients; 25% grade III—RLG, 27 remaining patients; 44% grade III, p = 0.05). Conclusion Patients with an isolated ramp lesion of the medial meniscus in association with an ACL injury displayed a higher amount of dynamic rotational laxity as expressed by the pivot shift test in comparison to patients with isolated ACL injury and no ramp lesion. The association between ramp lesions of the medial meniscus and increased pivot shift grading suggests that it is important to diagnose and repair them during ACL reconstruction surgery. Level of evidence III.

No abstract

Level II, diagnostic study--investigating a diagnostic test.

No abstract