MALDI-TOF mass spectrometry following short incubation on a solid medium is a valuable tool for rapid pathogen identification from positive blood cultures

Kohlmann, R.; Hoffmann, Alexander; Geis, G.; Gatermann, Sören

doi:10.1016/j.ijmm.2015.04.004

Cited by 72 publications

(47 citation statements)

References 42 publications

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“…Preliminary identification of the isolates by mass spectrometry with the MALDI-TOF system (Bruker Daltonics, Bremen, Germany) was performed simultaneously from the same material. 10 When the Gram stain revealed the presence of gram-negative diplococci and the preliminary identification by mass spectrometry corresponded to Acinetobacter spp, we adapted the modification of the Carba NP test proposed by Dortet et al 11 for the detection of OXA-carbapenemases to the RBCT. Briefly, the inoculum was re-suspended in two tubes, one containing 100 μl of the test solution (bromothymol blue/ZnSO 4 ) plus 100 μl 5 M NaCl and the other containing the same solutions plus imipenem.…”

Section: Methodsmentioning

confidence: 99%

Rapid Blue-Carba test: reduction in the detection time of carbapenemases performed from a 4-hour bacterial lawn

Nastro

Ayora

García

et al. 2016

Journal of Chemotherapy

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The increase in carbapenem-resistant gram-negative bacteria is a matter of concern due to the limited therapeutic options available. In severe infections caused by these isolates, the rapid detection of the mechanisms of resistance is vital. We described a slightly modified version of the Blue-Carba test, rapid Blue-Carba test, which allows the detection of carbapenemases at 4 h of incubation from a haze of bacterial growth obtained from a positive blood culture. It was able to detect carbapenemase-producing isolates (Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii) with a sensitivity and specificity of 98.1 and 100%, respectively. It is a rapid, easy-to-perform and an inexpensive technique that can be applied to routine laboratories, together with the simultaneous identification by mass spectrometry which would help to screen non-enzymatic carbapenem resistance; this method allows the detection of clinically relevant multidrug-resistant bacteria and the early implementation of accurate therapeutic interventions.

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Section: Methodsmentioning

confidence: 99%

Rapid Blue-Carba test: reduction in the detection time of carbapenemases performed from a 4-hour bacterial lawn

Nastro

Ayora

García

et al. 2016

Journal of Chemotherapy

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“…Direct testing of positive blood culture bottles by MALDI-TOF MS yields accurate identification; however, this approach requires the processing of the blood culture bottle contents, is not U.S. Food and Drug Administration (FDA) approved/cleared, and has a lower success rate than testing of colony isolates (4,6). As an alternative, the use of MALDI-TOF MS for the identification of bacterial isolates from positive blood cultures after short-term incubation (i.e., 2 to 6 h) of high-inoculum subcultures on solid media has been adopted by many laboratories, including our own (7)(8)(9). For example, we use this approach to test positive blood culture bottles showing Gram-negative bacilli upon Gram staining; in our hands, this method has excellent performance, enabling the identification of organisms in 92% (45/49) of blood culture bottles positive for Gramnegative bacilli following a 4-h incubation (our unpublished data).…”

Section: Rapid Testing Of Positive Blood Culture Bottlesmentioning

confidence: 99%

Syndromic Panel-Based Testing in Clinical Microbiology

et al. 2018

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The recent development of commercial panel-based molecular diagnostics for the rapid detection of pathogens in positive blood culture bottles, respiratory specimens, stool, and cerebrospinal fluid has resulted in a paradigm shift in clinical microbiology and clinical practice. This review focuses on U.S. Food and Drug Administration (FDA)-approved/cleared multiplex molecular panels with more than five targets designed to assist in the diagnosis of bloodstream, respiratory tract, gastrointestinal, or central nervous system infections. While these panel-based assays have the clear advantages of a rapid turnaround time and the detection of a large number of microorganisms and promise to improve health care, they present certain challenges, including cost and the definition of ideal test utilization strategies (i.e., optimal ordering) and test interpretation.

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“…For the diagnosis of bacterial pathogens responsible for bloodstream infections the mean incubation time to identify Gram-positive bacteria was 5.9 h, which dropped to 3.1 h if a crude acidic extract was prepared [52]. In other studies, 97 to 69.5% of bacteria were correctly identified after a short incubation of positive blood cultures on solid media of only 3 to 5 h [53][54][55][56], which enabled identification on the same day as the positive blood sample was detected. By increasing the sample concentration (by reducing the sample spot size), creating reference spectra for different cell densities, and immunoaffinity enrichment of bacteria, it was possible to detect as few as 10 to 100 bacterial cells after a blood culture time of only 4 h [20].…”

Section: Shortening the Cultivation Time For Faster Maldi-tof Ms Idenmentioning

confidence: 96%

MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain

Drissner

Freimoser

2017

Chem. Biol. Technol. Agric.

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Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS; MALDI biotyping) has become a standard tool for the accurate, rapid, and economical identification of pathogens in the clinical diagnostics laboratory. The method is continuously being improved, and new applications for distinguishing strains, identifying metabolites or functional characteristics (e.g., antibiotic resistance), and detecting microbes directly in patient samples have been developed. Adopting these methods in other disciplines than clinical diagnostics, for example, in agriculture, food safety and quality testing, or ecology, will open up new opportunities for diagnostics and research. This review focuses on MALDI-TOF MS approaches for the identification of yeasts and filamentous fungi. In contrast to bacterial diagnostics, MALDI biotyping of fungi is more challenging and less established. We thus start by discussing the role of MALDI-TOF MS as a tool for species identification; in particular with respect to DNA-based identification methods. The review then highlights the value of custom-made reference spectra for MALDI biotyping and points out recent advancements of MALDI-TOF MS, mainly from the field of clinical diagnostics that may be adopted and used for fungal diagnostic challenges. The overview ends with a summary of MALDI-TOF MS studies of yeasts and filamentous fungi of agricultural relevance.

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MALDI-TOF mass spectrometry following short incubation on a solid medium is a valuable tool for rapid pathogen identification from positive blood cultures

Cited by 72 publications

References 42 publications

Rapid Blue-Carba test: reduction in the detection time of carbapenemases performed from a 4-hour bacterial lawn

Rapid Blue-Carba test: reduction in the detection time of carbapenemases performed from a 4-hour bacterial lawn

Syndromic Panel-Based Testing in Clinical Microbiology

MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain

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