The PNC method detects different stages of caries lesions in real time, and it exceeds x-rays in sensitivity, without any ionizing radiation. Preliminary results showed a high potential of using the PNC method in clinical practice (98% accuracy) in comparison to the other methods.
In this research results of approbation of the optical PNC-method in dental clinic are presented The PNC-method was used for diagnostics stages of caries (initial, moderate and deep). The variant of the PNC-method [1-3] adapted for dental diagnosis is based on simultaneous analyses the following parameters by special algorithms: backscattering and probing radiation, stimulated (endogenous) auotofluorescence of caries induced batteries. Analyze of informational signals show good correlation with tooth morphological structure and concentration of anaerobic microflora in hearth of caries lesion.Investigation was performed in vivo on 101 tooth in conditions of typical dental clinic. Comparison of the PNC-method with visual and X-ray ones, which are widely used in clinical practice was made. Preliminary results showed high potential of usage the PNC-method in clinical practice and more high probability of initial canes detection (up to 100%) in comparison with X-ray method (approximately 75%). In cases when X-ray diagnosed absence of initial caries, more sensitive the PNCmethod detected initial caries in stage "white lesion".
Stimulation of production of reactive oxygen intermediates (ROI) was examined in human peripheral blood monocytes by luminol-dependent chemiluminescence. The dose-response curve characterizing the dependence of ROI production on the concentration of platelet-activating factor (PAF) showed that stimulation occurred within a concentration range of 2 x 10(-9)M to 5 x 10(-6)M. Transformation of the dose-response curve to an Eadie-Hofstee plot indicated that the process is characterized by two Km values. The Km value corresponding to the high-affinity branch of the curve is 1.3 +/- 0.14 nM. In the same cells, the dissociation constant for the [3H]PAF/receptor complex was determined. The Kd value was 0.8 +/- 0.1 nM, which agreed quite well with the high-affinity Km value obtained in the Eadie-Hofstee plot. The data indicate that stimulation of ROI generation is mediated through PAF binding at specific receptor sites at nanomolar PAF concentrations. Along with the specific receptor-mediated ROI generation, a nonspecific effect of PAF at a high concentration was demonstrated.
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