Biopsy or photodynamic therapy oftumors are usually investigated by fluorescent diagnostics methods.Information on modified method of fluorescence diagnostics of inflammatory diseases is represented in this research (optical PNC-method). Anaerobic microorganisms (bactenas) are an often cause of these pathological processes. These microorganisms also accompany disbiotic processes in intestines.Conducted reserches on studying the possibility of registration of monoculture of anaerobic microorganisms and their mixtures by the method of laser PNC-diagnostics. Stoke's and anti-Stoke's characteristics of 7 anaerobic and aerobic microorganisms (bacterias) types were researched.A choice ofthe listed bactenas as objects of study connected with that these species of microorganisms, presenting a normal microbiocenosis and being obligatory anaerobes are the most often cause of purulent-inflamatory diseases of different localizations.Moreover, at present very few facts are known about fluorescence of these bacterias under excitement by red light.
The PNC method detects different stages of caries lesions in real time, and it exceeds x-rays in sensitivity, without any ionizing radiation. Preliminary results showed a high potential of using the PNC method in clinical practice (98% accuracy) in comparison to the other methods.
In this research results of approbation of the optical PNC-method in dental clinic are presented The PNC-method was used for diagnostics stages of caries (initial, moderate and deep). The variant of the PNC-method [1-3] adapted for dental diagnosis is based on simultaneous analyses the following parameters by special algorithms: backscattering and probing radiation, stimulated (endogenous) auotofluorescence of caries induced batteries. Analyze of informational signals show good correlation with tooth morphological structure and concentration of anaerobic microflora in hearth of caries lesion.Investigation was performed in vivo on 101 tooth in conditions of typical dental clinic. Comparison of the PNC-method with visual and X-ray ones, which are widely used in clinical practice was made. Preliminary results showed high potential of usage the PNC-method in clinical practice and more high probability of initial canes detection (up to 100%) in comparison with X-ray method (approximately 75%). In cases when X-ray diagnosed absence of initial caries, more sensitive the PNCmethod detected initial caries in stage "white lesion".
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