Background/aim: Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation. The study aimed to assess serum 14-3-3eta, anti-CarP, and anti-Sa in seronegative RA (SNRA) patients who were treatment-naïve as well as in healthy subjects. This is the first study in the literature to examine these autoantibodies together in SNRA patients. Materials and methods: Forty-five treatment-naïve SNRA patients and 45 healthy subjects were recruited. Drugs change the levels of autoantibodies; therefore, patients who took any medication had been excluded from our study. Anti-carbamylated protein, anti-Sa, and 14-3-3eta were measured by using three different ELISA kits. Results: Median serum concentration of healthy controls in 14-3-3eta was 0.02 (0.02-0.27) ng/mL. Median serum concentration of SNRA patients in 14-3-3eta was 1.00 (0.48-1.28) ng/mL. Data were analyzed with Mann-Whitney U tests; the P-value was <0.001 in 14-3-3eta. Receiver operating characteristic (ROC) curve analysis showed that 14-3-3eta in SNR compared to healthy controls had a significant (P < 0.001) area under the curve (AUC) of 0.90 (95% confidence interval, 0.83-0.96). At a cutoff of ≥0.33 ng/mL, the ROC curve yielded a sensitivity of 88.9%, a specificity of 82.2%, a positive predictive value of 83.3%, and a negative predictive value of 88.1%. Conclusion: We found that 14-3-3eta can be used as a diagnostic marker in SNRA.
Objectives: In this study, we evaulated the distribution and alteration of hepatitis C virus (HCV) genotypes throughout years which has a clinical importance in the treatment and follow-up. Materials and Methods: Test results obtained from blood samples sent to the molecular microbiology laboratory at Aydın State Hospital for HCV genotype determination were analyzed retrospectively. A total of 182 samples collected between 2014 and 2018 were enrolled in the study. The determination of genotype and viral load of the samples were performed by real time polymerase chain reaction. Results: 53.8% (98/182) of the samples were collected from male patients and 46.2% (84/182) from female patients. The mean age of the patients was 58.5±15.5 years. 69.2% of the samples were genotype 1b, 18.1%-genotype 1a, 2.2%-genotype 1 (those different from subtype 1a and 1b), 1.7%-genotype 2, 7.2%genotype 3, and 1.7% of the samples were genotype 4. Conclusion: In the present study, genotype 1 was the most common genotype (89.5%). Additionally, we have observed a decrease in the frequency of genotype 1b and a slightly increase in the frequency of other genotypes. Determination of HCV genotypes is important for treatment and prognosis of HCV infections.
virus in chronic gastritis 211 Parkin gene in colorectal cancer 239 Ledipasvir and sofosbuvir in chronic hepatitis C 246 HBV and HCV are the risk factors in CCA 257 Assessment of incidental appendectomy specimens 264 Lanthanum Citrate influences the SMMC-7721 cells Real-life experience of ledipasvir and sofosbuvir single-tablet regimen among chronic hepatitis C patients in Turkey See page 239
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