ABSTRACT. The objective of this study is to establish an in vitro germination and cultivation protocol for murici (Byrsonima cydoniifolia A. Juss.) using zygotic embryos. Therefore, three assays were performed: in assay I, embryo asepsis was tested at exposure times of 5, 10, 15, 20, 25, and 30 minutes in 2.5% sodium hypochlorite, with or without immersion in 70% alcohol; in assay II, MS (MURASHIGE; SKOOG, 1962) e WPM (LLOYD; McCOWN, 1980) culture media were tested at salt concentrations of 25, 50, and 100%, with or without the addition of sucrose, to germinate the buds; in assay III, seedling growth was evaluated in MS and WPM culture media at salt concentrations of 25, 50 and 100%. Sodium hypochlorite (2.5%) with or without 70% alcohol was used to avoid contamination because it was not toxic to murici embryos. Water-agar was the most appropriate culture medium for bud germination, and 50% WPM was appropriate for seedling growth.Keywords: tissue culture, asepsis, culture medium, Cerrado fruit.Cultivo in vitro de embriões zigóticos de Murici (Byrsonima cydoniifolia A. Juss.): estabelecimento, desinfestação e germinação RESUMO. Objetivou-se com este estudo, estabelecer um protocolo de germinação e cultivo in vitro de murici (Byrsonima cydoniifolia A. Juss.) a partir de embriões zigóticos. Para isso, foram feitos três ensaios: no ensaio I, testou-se a assepsia do embrião, nos tempos de exposição de 5, 10, 15, 20, 25 e 30 minutos no hipoclorito de sódio a 2,5% com e sem a imersão em álcool 70%. No ensaio II, para a germinação de embriões, testou-se os meios de cultivo MS (MURASHIGE; SKOOG, 1962) e WPM (LLOYD; McCOWN, 1980) nas concentrações de sais de 25, 50 e 100%, com e sem a adição de sacarose. No ensaio III, avaliou-se o crescimento de plântulas em meios de cultivo MS e WPM nas concentrações de sais de 25, 50 e 100%. Verificou-se que a imersão de 1 minuto em álcool 70% e 25 minutos em hipoclorito de sódio (2,5%) foi adequado para evitar contaminações não sendo fitotóxico para o embrião de murici. Para a germinação dos embriões, o meio de cultivo com água-ágar é o mais adequado e para o crescimento de plântulas é o meio de cultivo WPM 50%.Palavras-chave: cultura de tecido, assepsia, meio de cultivo, frutífera do cerrado.

Hyptis marrubioides (Lamiaceae) is a medicinal plant that is native from Brazilian Cerrado. In vitro propagation techniques make use of elicitors to alter metabolic pathways, affecting how molecules are produced both qualitatively and quantitatively. This research aimed to evaluate how abiotic elicitors salicylic acid (SA) and silver nitrate (SN) at concentrations of 30µM or 60µM influence Hyptis marrubioides seedling growth by two different in vitro culture methods. The rutin content was quantified by HPLC-DAD. Compared to an untreated culture, the H. marrubioides methanolic extracts cultured in MS medium for 10 days followed by culture in MS medium containing SN (30µM) for 20 days had 1.28 times higher rutin content. In a second experiment, seedlings were cultured in MS medium for 20 days, and then the desired elicitor was added to the culture and allowed to remain in contact with the medium for three and six days. SA (30µM) gave the best results: rutin production was 16.56-foldhigher than the control after six days. SN (30µM) increased the rutin content by 1.17-fold. At the two concentrations evaluated during the elicitation experiments, neither SA nor SN altered the growth parameters shoot length, leaf number, and fresh and dry weight of H. marrubioides seedlings grown in vitro as compared to the control. Based on these results, the abiotic elicitors SA and SN successfully provide Hyptis marrubioides with increased rutin content in vitro.

Physalis angulata Linn. is a plant with great importance in folk medicine for its various therapeutic properties and the production of active compounds. It is known as camapú in Brazil. The P. angulata seedlings were cultured in vitro under different light qualities such as white (control), blue, green, red, and yellow at 16 h photoperiod. After 30 days of culture, the shoot length, number of leaves, fresh and dry matter and rutin content were evaluated in triplicate in methanol extracts of seedlings exposed to the different lights by High-Performance Liquid Chromatography-Diode Array (HPLC-DAD). The mean shoot length was longer in seedlings cultured under yellow light (22.83 ± 0.65 cm, 1.62-fold), red light (22.58 ± 0.44 cm, 1.6-fold), or green light (20.57 ± 0.72 cm, 1.46-fold) than seedlings exposed to white light (14.13 ± 0.26 cm). There were no differences in the mean number of leaves between seedlings grown under the remaining lights and white light. Fresh (1,152 ± 0.16 g) and dry weight (0.078 ± 0.01 g) were higher in seedlings grown under white light. However, rutin production was higher under blue light (2.78 ± 0.05 μg g-1 by dry weight) and green light (2.40 ± 0.06 μg g-1 by dry weight). Therefore, the various light qualities affected the growth of P. angulata seedlings differently under in vitro culture condition. The blue and green lights promoted greater accumulation of rutin in this species.

No abstract

No abstract