Caffeine induces loss of calcium and influences the normal development of bone. This study investigated the effects of coffee on bone metabolism in rats by biochemical measurement of calcium, bone densitometry and histometry. Male rats, born of female treated daily with coffee and with coffee intake since born, were anesthetized, subjected to extraction of the upper right incisor, and sacrificed 7, 21 and 42 days after surgery. Blood and urine samples were taken, and their maxilla radiographed and processed to obtain 5-µm-thick semi-serial sections stained with hematoxylin and eosin. The volume and bone quality were estimated using an image-analysis software. The results showed significantly greater amount of calcium in the plasma (9.40 ± 1.73 versus 9.80 ± 2.05 mg%) and urine (1.00 ± 0.50 versus 1.25 ± 0.70 mg/24 h) and significantly less amount in bone (90.0 ± 1.94 versus 86.0 ± 2.12 mg/mg bone), reduced bone mineral density (1.05 ± 0.11 versus 0.65 ± 0.15 mmAL), and lower amount of bone (76.19 ± 1.6 versus 53.41 ± 2.1 %) (ANOVA; p≤0.01) in animals treated with coffee sacrificed after 42 days. It may be concluded that coffee/caffeine intake caused serious adverse effects on calcium metabolism in rats, including increased levels of calcium in the urine and plasma, decreased bone mineral density and lower volume of bone, thus delaying the bone repair process.

Objective: Patients using a removable prosthesis are susceptible to a variety of oral lesions that may progress to cancer. Toluidine blue (TB) staining is used to identify premalignant lesions, but the results are still controversial. Since micronuclei (MN) are a biomarker of genetic instability, the objective of this study was to determine the frequency of MN in white lesions of the oral mucosa and to compare the results with those of the TB test. Study Design: The study included 20 removable prosthesis users with white lesions that were previously classified as toluidine positive or negative. The frequency of MN was evaluated in exfoliated cells from lesions and normal mucosa. Nuclear anomalies were also registered. Results: A significant increase (p < 0.05) in the frequency of MN was observed in exfoliated cells from lesions compared to normal mucosal cells, and no relationship was seen with TB staining. Lifestyle factors or gender did not influence the results. Conclusions: The frequency of MN is a sensitive biomarker and can be used to predict genomic instability in white oral lesions. The MN assay may serve as a good parameter in the battery of tests used to identify high-risk individuals, contributing to the identification of the biological conditions of oral lesions.

Osteoblastic cells associated with autogenous bone accelerated the repair of bone defect, and the action of the osteoblastic cells was more effective until the 10th day and of the autogenous bone after this period.

Para a formação dos cirugiões-dentistas, é de grande importância o atendimento de pacientes durante o período de graduação, por isso, algumas Faculdades oferecem atendimento gratuito a pacientes sob a supervisão de um professor. Muitas avaliações são realizadas para verificar a qualidade do atendimento, no entanto, deixam de lado a visão dos pacientes que exercem um papel fundamental. Tendo em vista a importância do usuário para o processo de ensinoaprendizagem, o objetivo desse trabalho foi avaliar asatisfação dos pacientes com o serviço odontológico prestado pelas clínicas na Universidade de Franca, para assim poder analisar os aspectos do atendimento, e desenvolver novos métodos visando propiciar ao paciente um atendimento adequado e de qualidade. Foram entrevistados 81 pacientes, com o auxílio de um questionário proposto por Davies e Ware Jr. (1982) e adaptado para esta pesquisa, sendo compostaspor 18 questões que permitiram avaliar o acesso, disponibilidade ou conveniência, custo, continuidade, satisfação geral, controle da dor, qualidade, acesso total e índice de satisfação com o atendimento odontológico, através de uma regra de escores. Diante dos resultados apresentados, encontrou-se que a maioria das questões apresentou mais de 70% de respostas positivas, no entanto, o item relativo à satisfação geral, que engloba apenas uma pergunta, ondeo paciente diz se o atendimento poderia ser melhor, recebeu um percentual menor (56%). Pode-se concluir que os pacientes apresentaram-se parcialmente satisfeitos com o atendimento realizados pelas clínicas da Universidade de Franca.

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