Osteogenic Potential of Autogenous Bone Associated with Bone Marrow Osteoblastic Cells in Bony Defects: A Histomorphometric Study

Lacerda, Suzie Aparecida de; Lanzoni, João Fernando Arnoni; Bombonato-Prado, Karina Fittipaldi; Campos, Alessandra Aparecida; Prata, Celina Antonio; Brentegani, Luiz Guilherme

doi:10.1097/id.0b013e3181b8e53c

Cited by 12 publications

(6 citation statements)

References 35 publications

(20 reference statements)

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“…Cell-cell interactions between host and donor cells are believed to play an important role in enhancing the healing process after in vivo cell transplantation (Watanabe et al, 1999). In cell therapy for bone defect healing, the delivery of osteogenic cells into the defect area is often considered to be of direct help to the new bone formation (Lacerda et al, 2009). Due to reduced multipotency compared with progenitor cells, osteoblasts may be more committed to form new bone tissue (Leach and Mooney, 2004).…”

Section: Introductionmentioning

confidence: 99%

Implantation of osteogenic differentiated donor mesenchymal stem cells causes recruitment of host cells

Zhou

Wei

Prasadam

et al. 2012

J Tissue Eng Regen Med

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The interaction between host and donor cells is believed to play an important role in osteogenesis. However, it is still unclear how donor osteogenic cells behave and interact with host cells in vivo. The purpose of this study was to track the interactions between transplanted osteogenic cells and host cells during osteogenesis. In vitro migration assay was carried out to investigate the ability of osteogenic differentiated human mesenchymal stem cells (O-hMSCs) to recruit MSCs. At the in vivo level, O-hMSCs were implanted subcutaneously or into skull defects in severe combined immunodeficient (SCID) mice. New bone formation was observed by micro-CT and histological procedures. In situ hybridization (ISH) against human Alu sequences was performed to distinguish donor osteogenic cells from host cells. In vitro migration assay revealed an increased migration potential of MSCs by co-culturing with O-hMSCs. In agreement with the results of in vitro studies, ISH against human Alu sequences showed that host mouse MSCs migrated in large numbers into the transplantation site in response to O-hMSCs. Interestingly, host cells recruited by O-hMSCs were the major cell populations in newly formed bone tissues, indicating that O-hMSCs can trigger and initiate osteogenesis when transplanted in orthotopic sites. The observations from this study demonstrated that in vitro induced O-hMSCs were able to attract host MSCs in vivo and were involved in osteogenesis together with host cells, which may be of importance for bone tissue-engineering applications.

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Section: Introductionmentioning

confidence: 99%

Implantation of osteogenic differentiated donor mesenchymal stem cells causes recruitment of host cells

Zhou

Wei

Prasadam

et al. 2012

J Tissue Eng Regen Med

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“…Yet, the method has its limitations, such as the limited availability of tissues, the increased period of time and interventions especially in the case of reconstructions (Misch, 2011). Alternative methods were researched, from which the use of bone marrow aspirate was studied to fill the bone defects to stimulate the healing of fractures and to treat pseudoarthrosis (Lacerda et al, 2009). Using a bone marrow aspirate for the treatment of bone defects on dogs, the improvement of bone formation was obtained (Niemeyer et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

The Influence of Haematogenous Bone Marrow on the Early Osseointegration of a Titanium Implant which Penetrates the Endosteum

Rațiu¹,

Raţiu²,

Mitre³

et al. 2022

Int. J. Morphol.

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The utility of metallic bio-medical implants in osseous or dental affections is irrefutable. The paper aims to test the tolerance of the bone marrow to titanium implants. Titanium implants were inserted in the femur of 11-months old rabbits. The implants penetrated the endosteum, half of their length getting into the haematogenous bone marrow. Seven days after the insertion we collected bone fragments containing the implant. The CT exam revealed a significant decrease in the density of the bone at the interface with the implant and a more discrete one aloof from the insertion area. The histologic exam after 7 days revealed osseous reparatory processes only in the endosteal area from where it expanded on the surface of the implant which was inside the marrow. The presence and intensity of the osseous reparatory processes after only seven days post-implant demonstrates that the marrow actively participates in bone regeneration and implants osseointegration.

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“…Moreover, transplant of the autogenous bone block resulted in an intrabony-like space with suffi cient exposure of the bone marrow, which was suitable for fi lling and maintaining particulate bone substitutes and provided better vascularization for bone regeneration. 7 In compromised clinical situations, it is recommended that implants be placed several months after bone grafting because their placement at the time of tissue grafting is appropriate only when optimal positioning can be achieved. 8 A recent systemic review concluded that survival rates of implants placed into augmented areas were comparable with that of implants placed into pristine bone.…”

Section: Discussionmentioning

confidence: 99%

Localized Bone Augmentation and Implant Site Development-Review with a case

Koirala

Pradhan

Gorkhali

2019

J. Nep. Prosthodon. Soc.

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Guided bone regeneration (GBR) has been used for the regeneration of bone in conjunction with the placement of dental implants, for augmentation of resorbed alveolar crests, and to treat localized ridge deformities. It is based on the principle of protecting bone regeneration against overgrowth of tissues formed by rapidly proliferating non-osteogenic cells. In this case, the space created by the Titanium mesh supported platelet rich fibrin membrane was filled by tissues with features of newly formed bone. No residual bone defects were observed and an increase of the alveolar width and height was observed. No untoward effects on bone regeneration were observed except membrane exposure after 4 and 1/2months. This case shows a satisfactory result concerning GBR technique or implant site development.

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Osteogenic Potential of Autogenous Bone Associated with Bone Marrow Osteoblastic Cells in Bony Defects: A Histomorphometric Study

Abstract: Osteoblastic cells associated with autogenous bone accelerated the repair of bone defect, and the action of the osteoblastic cells was more effective until the 10th day and of the autogenous bone after this period.

Cited by 12 publications

References 35 publications

Implantation of osteogenic differentiated donor mesenchymal stem cells causes recruitment of host cells

Implantation of osteogenic differentiated donor mesenchymal stem cells causes recruitment of host cells

The Influence of Haematogenous Bone Marrow on the Early Osseointegration of a Titanium Implant which Penetrates the Endosteum

Localized Bone Augmentation and Implant Site Development-Review with a case

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