The purpose of the research work was to select the optimal conditions for the cultivation of microorganisms. As a result of the conducted research work, the modes of growing a nitrogen-fixing culture and a microorganism with high enzymatic activity were selected and worked out. At the same time, the optimal conditions for the cultivation of Azotobacter sp were determined – the temperature optimum for cell accumulation was 30°C, for increased polysaccharide production 20 °C, aeration within 5-10 l/l/min, agitator speed-150 rpm, pH value within 6.0±0.2 units, which allowed to achieve a cell titer of at least 1.0×109 CFU/ml. A cost-effective nutrient medium was selected for growing Pseudomonas sp. molasses-autolysate medium and optimal conditions for growing the culture: cultivation temperature 30-32 °C, aeration 1.0-1.5 l/l/ min, agitator speed 150-200 rpm, pH 6.8-7.2 units, sub-titration 5.0 % KOH, defoaming with adecanol, cultivation time-72 hours, which allowed to achieve a cell titer of at least 1.0×109 CFU/ml.
This publication presents the results of studying the biosafety of microorganisms used for the development of a biological product-a litter destructor, both individually and in a joint composition. In the experiment, the culture of Stylonychia (Stylonychia mytilus) and laboratory animals (rabbits, mice, and rats) were used. Based on a series of preclinical studies, it was found that the studied microorganisms individually do not indicate any symptoms of virulence, toxigenicity, toxicity and do not give an affirmative counteraction to the formation of hemolysin. The results of the express method showed that the aqueous and acetone extracts obtained from the joint microbial composition did not have a detrimental effect on the culture of protozoa, the viability in all groups was 100.0%. The analysis of the dermonecrotic effect of the studied microbial preparation demonstrated that the studied extracts did not cause hemorrhage, hyperemia, peeling, edema, and other pathology indicating an inflammatory process on the skin of laboratory rabbits. Conducting an acute experiment confirmed the safety of the studied microbial consortium, since during the research the laboratory mice remained active, mobile, no signs of poisoning or other pathology were found, and the conducted pathoanatomical autopsy did not reveal lesions of the parenchymal organs of the laboratory test objects. In general, the results of the studies indicated that the investigated cultures of microorganisms and their joint composition in the composition of the biological product-destructor do not show a toxic impact on the body of lab test objects, which confirms their harmlessness.
Современными молекулярно-биологическими и микробиологическими методами анализа изучена возможность использования в товарном производстве бактериальных штаммов рода Lactobacillus, полученных из содержимого слепых отростков ЖКТ дикого перепела. Штаммы выделены на селективных плотных средах, типированы до вида при определении последовательности гена 16S РНК. Четыре штамма рода Lactobacillus идентифицированы как принадлежащие к видам L. parabuchneri, L. brevis, L. buchneri и L. kunkeei. Проведёны их морфологический анализ и определены культуральные свойства; молекулярное типирование методами ERIC-ПЦР и (GTG)5-ПЦР; экспресс-анализ на наличие в тотальной ДНК 4 отобранных штаммов лактобацилл генов, кодирующих пептиды, подобные педиоцину, низину и энтероцину. В отношении штаммов L. brevis (В-13079) и L. buchneri (В-13078) проведён комплекс экспериментов, подтверждающий возможность использования их в составе пробиотических препаратов. На основе комплекса исследований предложен состав пробиотической добавки «Олигобакт-ДТ», которую можно применять в рационах сельскохозяйственной птицы.
This article presents the results of a study of the effect of a feed additive "Albit-BIO", on the body quail meat productivity. This feed additive made from autolysate of the biomass of Cephaliophora Tropica fungus, saturated with selenium, iodine, iron, calcium, magnesium, zinc, manganese, copper, cobalt, and boron. As a result of the studies, found that the addition in the diet of quail feed additive helps increase poultry safety by 3.5 %, live growth by 6.6 % while reducing feed consumption for growth by 1.5 %. Revealed that the use of autolysate from the fungus in the diet of birds helps increase protein, mineral, and fat metabolism, and increases the quail's immune status. The feed additive had a positive effect on the digestibility of the feed components, stimulated the growth of beneficial microflora, and inhibition of growing the opportunistic microorganisms. The use of additives helps improve the chemical composition of the bird's muscles by reducing the fat content and increasing protein, as well as increasing the dietary properties of meat products and its biological value. In general, the use of the feed additive Albit-BIO as part of the quail diet increases the meat productivity of poultry and improves the quality of the obtained quail products.
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