The aim of this study was to determine whether treatment with fish oil may alter the periapical bone resorption following pulp exposure in rats. Untreated and pulp-exposed animals served as the negative and positive control, respectively. Other pulp-exposed animals were orally treated with fish oil at different concentrations and frequencies. Periapical tissue sections were stained with tartrate-resistant acid phosphatase (TRAP), and then the numbers of both periapical osteodasts and preosteoclasts were determined. The levels of bone resorption were assessed using the osteoclast-bone interface (OBI) index. The results showed that no differences between the numbers of both osteoclasts and preosteoclasts in the fish oil-treated animals and the negative control at day 14 could be found. Similarly, the periapical bone resorption in the animals treated with fish oil for 14 days significantly reduced to the levels of that in the negative control. The results of the present study, therefore, suggest that oral treatment with fish oil may inhibit bone resorption following pulp exposure in rats and hence, may have a therapeutic modality for inflamed periradicular tissue.
Background: Demineralisation and remineralisation is a natural process in tooth enamel. It is influenced by the content of calcium and phosphorus in saliva, which concentrations are affected by the consumption of food, including formula milk. Demineralisation and remineralisation determine the roughness and hardness of the enamel surface. Purpose: This study compared the effect of formula milk on the roughness and hardness of tooth enamel. Methods: Maxillary premolar extracted teeth were demineralised with 37% phosphoric acid for 90 seconds and then divided into four treatment groups. For four days, the teeth were immersed twice a day in cow formula for five and ten minutes (Group I and II) and soy formula for five and ten minutes (Group III and IV). Before and after the immersion in milk, the teeth were submerged in artificial saliva. The enamel surface roughness and hardness were measured three times using a surface roughness tester and a Vickers microhardness tester, before and after demineralisation and after immersion in milk. Data were analysed using Kruskal–Wallis and post hoc Mann–Whitney tests. Results: There was no significant difference (p=0.88) observed in the roughness reduction among the treatment groups. The highest increase in hardness was noted for the ten-minute cow formula milk group (93.27 ± 16.00). The increase of hardness was higher after immersion for ten minutes. A substantial difference (p=0.03) was seen in the increase of hardness between the treatment groups. Conclusion: Immersion in cow and soy formula milk for five and ten minutes does not reduce the enamel roughness, but it increases the enamel hardness.
Background: Adolescence is a period when an individual experiences physical and psychological growth, thus requiring higher energy intake. As a result, they have a high appetite, but at the same time the supervision of parents on their oral hygiene behavior is decreases. They become free to choose their preferred food, sometimes containing high carbohydrates that may increase risks of dental caries and overweight. Sleman is one of districs in Yogyakarta, also considered as an agglomeration area of Yogyakarta town, which still has urban and rural areas. Purpose: This study aimed to examine the correlation between residence area and parental socioeconomic conditions with prediction to get a new dental caries. Method: This study was an analytic survey study conducted on 275 adolescents in Sleman. Samples were selected by using stratified cluster random sampling technique. Prediction to get a new dental caries was measured by using cariogram, involving 10 variables. Residence area was observed based on territorial characteristics, such as urban and rural areas matched to their ID card. Meanwhile, parental socio-economic condition was measured on daily expenses of their parents. A multiple regression analysis with dummy variables was used to analyses the correlation between the independent and dependent variables at a confidence level of 95%. Result: The results showed that the prevalence of caries in those adolescents in urban areas was 70.7%, while 81.95% in rural areas. The DMFT index in urban areas was 2.27, while 2.65 in rural areas. The mean percentage of prediction to get a new dental caries in urban areas was 47.83 ± 23.63, while 53.61 ± 24.68 in rural areas. The results of the statistical analysis then showed that there was no significant correlation of residence area and parental sosio-econimic conditions with prediction to get a new dental caries. Conclusion: In conclusion, residence areas, rural and urban areas, and parental socio-economic conditions, from low to high economic status were not correlated with prediction to get a new dental caries in adolescents.
Streptococcus mutans plays an important role in the pathogenesis of caries. This bacteria has virulence properties involve in the formation of biofilm on tooth surface. Due to its antibacterial effect, Jamblang leaf may be used as an agent to prevent caries. Objective: This study aimed to elucidate the effects of ethanolic extract of Jamblang leaves on the growth of S. mutans. Methods: Analysis of active compounds was carried out using thin layer chromatography (TLC) and Liquid Chromatograph-Mass Spectrography (LC-MS). Samples of S. mutans were isolated from children’s carious deciduous molar teeth. Growth test was done by dilusion technique. Aquadest was used as a negative control. Results: TLC and LC-MS showed the presence of the flavonoid, tannin, and terpenoid Kruskall-Wallis test showed significant differences (p<0.05) among the groups, indicating that Jamblang leaves ethanolic extract decreased the growth of S.mutans. The higher concentrations of the extract, the less number of S.mutans colonies grown. No colony of S. mutans at 22.5% of extract’s concentration. Conclusion: Jamblang leaves ethanolic extract reduces the growth of S. mutans. Concentration of 22.5%Jamblang leaves ethanolic extracthas bacteriocid effect. Key words: Ethanolic extract of Jamblang (Syzygium cumini) leaves, growth, Streptococcus mutans
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