The inhibitory activity of angiotensin I-converting enzyme in milk increased during fermentation with the Calpis sour milk starter containing Lactobacillus helveticus and Saccharomyces cerevisiae. Two kinds of peptides inhibitory to angiotensin I-converting enzyme were purified from the sour milk by using four-step HPLC. The amino acid sequences of these inhibitors were identified as Val-Pro-Pro and Ile-Pro-Pro. The concentrations of peptides providing 50% inhibition of angiotensin I-converting enzyme were 9 and 5 microM, respectively. Most of the inhibitory activity in sour milk was attributed to these two peptides.
We compared a variety of models for the analysis of data on the wind dispersal of seeds and pollen. Dispersal distances from a source depend upon such factors as settling velocity, height of release, wind speed and turbulence, and specific morphological adaptations for dispersal. The dispersal curve, which describes the frequency distribution of dispersal distances, usually shows its peak at some distance from a source and falls off with distance. We used the location of that peak as a measure of dispersal, and organized data in terms of the predictions of two models for the dynamics of advection and diffusion. These data are summarized in a seed dispersal diagram in which the modal distance normalized by the height of seed release is plotted against the mean with speed normalized by the falling speed of seeds. Data from 15 studies were used to compare actual dispersal relationships.
The timing of lytic phage development and the relationship between host generation times and latent periods were investigated by electron microscopy of one-step growth experiments in two strains of marine Vibrio species. Results were used in a correction factor developed to interpret field studies of phage-infected marine bacteria. Both the number of mature phage per average cell section and the percentage of cells with mature phage increased exponentially by 73-86% into the latent periods. Assuming that bacterial infection and lysis take place continually in the ocean, conversion factors for relating the percentage of visibly infected bacteria to the total percentage of the bacterial community that are phage-infected were calculated as 3.70-7.14. When this range of factors was applied to previously-collected field data [Proctor LM, Fuhrman JA (1990) Nature (Lond) 343:60-62; Proctor LM, Fuhrman JA (1991) Mar Ecol Prog Ser 69:133-142] from 3 to 31% of the free-living bacteria and 3 to 26% of particulate-associated bacteria appeared to be phage-infected at any given time. Based upon a steady-state model in which half the daughter cells survive to divide again, the percent of total mortality would be twice the total percentage of phage-infected cells. From 6 to 62% and from 6 to 52% of mortality for the free-living and particulate-associated bacterial community, respectively, may be due to viruses.
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