Cockroaches are among the most common pests in public dwellings and health facilities. Owing to their close association with man and all his surroundings they are considered aspotential carrier and transmitter of human diseases. In hospital, cockroaches are the possible vector of nosocomial infection, specially for the transmission of drug resistant bacteria. The aim of the present study was to identify the major cockroach species, to isolate common foodborne pathogens from cockroach and to determine the antibiotic susceptibility pattern of isolated bacteria. This case control study was conducted on cockroaches captured from hospital, restaurants and houses in Dhaka city, from a period of July to December 2014. A total of 450 cockroaches were collected. Among these 300 cockroaches (150 from hospital and 150 from restaurants) were considered as case and 150 cockroaches from houses were enrolled as control group. All (100%) cockroaches were identified as Blattellagermanica. Examination of external surface and gut homogenates showed that all (100%) cockroaches carried bacteria, while carriage rate of fungi and parasites were 28.2% and 3.7% respectively. Of 1901 isolated bacteria, Escherichia coli (15.3%), Pseudomonas aeruginosa (14.4%) and Salmonella spp. (12.9%) were the predominant bacteria. Other bacteria of medical relevance included, Shigella spp. (6.4%), Klebsiella spp. (8.8%), Proteus spp. (9.6%) and Staphylococcus aureus (4.3%). Antibiotic resistance pattern showed high resistance rate (62.4%) of bacterial isolates to different antibiotics. Additionally, 1051(55.3%) of the isolates were multi drug resistant (MDR) strains, which were resistant to 5-14 antibiotics. The findings of this study revealed that cockroaches are potential source of pathogenic microorganisms including multi drug resistant bacterial strains, which could be a major threat to public health. Hence, effective preventive and control measures are required to minimize cockroach related infections.
amikacin, moxifloxacin, imipenem, meropenem, piperacillin+tazobactum combination, vancomycin, doxycycline, tetracycline, tigecycline, nitrofurantoin, azactum, linezolid and 100% resistant to cefixime. Enterobacter was 100% sensitive to penicillin, amikacin, gentamicin, netilmicin, doxycycline, tetracycline, tigecycline and 100% resistant to cefixime, ceftazidime, ceftriaxone, cefepime, cotrimoxazole, levofloxacin, vancomycin. E. coli was 100% sensitive to imipenem, meropenem, vancomycin, tigecycline and 100% resistant to mecillinam, aztreonam. Klebsiella was 100% sensitive to flucloxacillin, colistin, vancomycin, tigecycline, linezolid and 100% resistant to nalidixic acid. Proteus was 100% sensitive to cephradine,
Background: Different bacterial enteropathogens are responsible for diarrhoea in children less than 5 years of age in Bangladesh. This study aimed to determine the antimicrobial sensitivity pattern of diarrhoeal pathogens that is necessary to know for the specific management of acute bacterial diarrhoea. Objectives: To find out the bacterial enteropathogens causing diarrhoea and their antimicrobial sensitivity pattern in under 5 children. Methodology: This was an observational cross sectional study carried out at Microbiology Department of Sir Salimullah Medical College, Dhaka. A total of 217 diarrhoeal patients 0-5 years age, attending Pediatric unit of Sir Salimullah Medical College and Mitford Hospital. Dhaka and Dhaka Shishu Hospital, Dhaka during the period of July, 2015 to June, 2016, were enrolled in the study. For isolation of the common enteric bacteria, diarrhoeal stools were cultured on MacConkey agar media and for Campylobacter spp, diarrhoeal stools were cultured on selective Modified Campy BAP media and nonselective Blood agar media with membrane filter. Positive culture was confirmed by Gram stain morphology and standard biochemical tests. Antimicrobial susceptibility test were used to identify the bacterial sensitivity pattern in the isolates. Results: Out of 217 diarrhoeal stools, bacteria was isolated from 97 (44.70%) patients by culture. Among 97 culture positive cases Eschericha coli (51- 52.58%), Campylobacter spp. (23-23.71%), Shigella spp. (15-15.46%) and Salmonella spp. (8-8.25%) were isolated. Mecillinam is the most sensitive drug for Escherichia coli, Campylobacter spp., Shigella spp. and Salmonella spp. Among the other antimicrobials, Campylabacter spp. were sensitive to azithromycin, erythromycin and nitrofurantoin. Escherichia coli were sensitive to nitrofurantoin, gentamicin and azithromycin. Shigella spp. were sensitive to ceftriaxone, ciprofloxacin and nitrofurantoin and Salmonella spp. were sensitive to ceftriaxone, cotrimoxazole and gentamicin. Conclusion: Mecillinam and nitrofurantoin were highly effective against bacteria causing diarrhoea in children. Bang Med J (Khulna) 2019; 52 : 35-39
Background: nfectious diarrhoea is a major public health problem in developing countries like Bangladesh. Bacteria and parasites are important etiological agents for diarrhoea in infants and children. Objectives: To isolate and identify the causative organisms of diarrhoea from diarrhoeal stools in under 5 children. Methodology: This was an observational cross sectional study carried out at Microbiology Department of Sir Salimullah Medical College, Dhaka. A total of 217 diarrhoeal patients upto 5 years age, attending Pediatric unit of Sir Salimullah Medical College and Mitford Hospital, Dhaka and Dhaka Shishu Hospital, Dhaka during the period of July 2015 to June 2016, were enrolled in the study. For isolation of enteric bacteria, diarrhoeal stools were inoculated on Mackonkey agar media and for Campylobacter spp. diarrhoeal stools were inoculated on selective Modified Campy-BAP media and nonselective Blood agar media with membrane filter. Positive culture was confirmed by Gram stain morphology and standard biochemical tests. Oocysts of Cryptosporidium were identified by Modified Ziehl Neelsen staining and other parasites were identified by wet mount preparation. Results: Out of 217 diarrhoeal stools bacteria were isolated from 97 (44.7%) patients. Among 97 cases, Escherichia coli were detected in 51(52.58%) patients; Campylobacter spp. were detected in 23(23.71%) patients; Shigella spp. were detected in 15(15.46%) patients and Salmonella spp. were detected in 8(8.25%) patients. Microscopic finding of diarrhoeal stools were detected by pus cells with or without RBCs in 90(70.31%) samples. Cysts of Giardia intestinalis were found in 9(4.14%) samples, cysts of Entamoeba histolytica in 2(0.9%) samples, eggs of Trituris trichiura in 2(0.9%) samples and oocysts of Cryptosporidium were found in.5(2.30%) samples. Conclusion: Escherichia coli was the most common bacteria and Giardia intestinalis was predominant parasite causing diarrhoea in under 5 children. Bang Med J (Khulna) 2018; 51 : 25-28
Nasal carriage of MRSA among hospital stuff act as a source of endogenous infection and becomes a source for hospital and community acquired infection. The study was conducted to determine the rate antibiotic resistance pattern of nasal carriage of MRSA among the hospital stuff of Sir Salimullah Medical College and Mitford Hospital, Dhaka. Pre moistened nasal swabs from hospital stuff (doctor, nurses, lab technicians and other helping stuff were obtained. These swabs were inoculated into Blood agar and Mannitol salt agar media. Antibiogram was done by modified Kirby Bauer disc diffusion method. MRSA were detected by oxacillin and cefoxitin disc diffusion method. The resistance was confirmed by MIC of oxacillin agar dilution method. Out of 142 samples 34 strains of Staphylococcus were isolated among them 07 (4.93%) were MRSA and 27 (19.01%) were MSSA. The carriage rate of MRSA was higher among nurse than other healthcare provider. Nasal carriage of MRSA is responsible for spreading infection from healthcare personnel to normal individual. So, regular screening of carrier is required from prevention of hospital acquired infection. Bangladesh J Med Microbiol 2017; 11 (2): 17-19
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