No abstract

Graphical abstract

No abstract

The taxonomic position of a novel actinomycete, strain RC 1831 T , isolated from the sediment of a fish dumping yard at Barkul village near Chilika Lake, Odisha, India, was determined by a polyphasic approach. Based on morphological and chemotaxonomic characteristics the isolate was determined to belong to the genus Streptomyces. The phylogenetic tree based on its nearly complete 16S rRNA gene sequence (1428 nt) with representative strains showed that the strain consistently falls into a distinct phyletic line together with Streptomyces glaucosporus DSM 41689 T (98.22 % similarity) and a subclade consisting of Streptomyces atacamensis DSM 42065 T (98.40 %), Streptomyces radiopugnans R97 DSM 41901 T (98.27 %), Streptomyces fenghuangensis GIMN4.003 T (98.33 %), Streptomyces nanhaiensis DSM 41926 T (98.13 %), Streptomyces megasporus NBRC 14749 T (97.37 %) and Streptomyces macrosporus NBRC 14748 T (98.22 %). However, the levels of DNA-DNA relatedness between strain RC 1831 T and phylogenetically related strains Streptomyces atacamensis DSM 42065 T (28.75±3.25 %) and Streptomyces glaucosporus DSM 41689 T (15±2.40 %) were significantly lower than the 70 % threshold value for delineation of genomic species. Furthermore, the isolate could be distinguished phenotypically on the basis of physiological, morphological and biochemical differences from its closest phylogenetic neighbours and other related reference strains. Strain RC 1831 T is therefore considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces barkulensis sp. nov. is proposed. The type strain is RC 1831 T (5JCM 18754 T 5DSM 42082 T ).

No abstract

No abstract

A novel actinobacterial strain RC1832T was isolated from the sediment of a fish dumping yard atBalugaon near Chilika Lake. The strain is halotolerant (15 % NaCl, w/v), alkali-tolerant (pH 7-10) and hydrolyzes chitin, starch, gelatin, cellulose, carboxymethyl cellulose,

moderately halophilic spore forming, motile, Gram-positive, rod-shaped bacterial strain designated as KGW1 was isolated from water sample of Chilika Lake and characterized taxonomically using polyphasic approach. The strain grew in the presence of 0-25% (w/v) NaCl in marine salt agar media, hydrolyzes casein, and gelatin and shows presence of alkaline proteases. The major cell wall menaquinone was MK7 and major cellular fatty acids were anteiso-C (44.89%), anteiso-C (6.18%), isoC (19.38%), and iso-C (7.39%). Several chemotaxonomic features conform the isolate be a member of genus Halobacillus. The isolate KGW1 contained A1γ meso-Dpm-direct type of peptidoglycan which is different from its phylogenetically closest neighbours. The 16S rRNA gene sequence based phylogenetic analysis also revealed the strain KGW1 was affiliated to the genus Halobacillus and sequence similarity between the isolated strain and the type strains of Halobacillus species were found closest to, H. dabanensis D-8 DSM 18199 (99.08%) and H. faecis IGA7-4 DSM 21559 (99.01%), H. trueperi SL-5 DSM 10404 (98.94%). The in silico DDH showed that the values in a range of 14.2-17.5% with the most closest strain H. dabanensis D-8 DSM 18199 and other type strains of the genus Halobacillus for which whole genome sequence is reported. DNA-DNA relatedness between strain KGW1 and the closest type strain Halobacillus trueperi DSM 10404 was 11.75% (± 1.15). The draft genome sequence includes 3,683,819 bases and comprises of 3898 predicted coding sequences with a G + C content of 46.98%. Thus, the significant distinctiveness supported by phenotypic and genotypic data with its closest neighbors and other closely related species confirm the strain KGW1 to be classified as a novel species within the genus Halobacillus, for which the name Halobacillus marinus sp. nov. is proposed. The type strain is KGW1 (= DSM 29522 = JCM 30443).