Material and Methods: This is an experimental study with pre-test pos-ttest group design carried out by purposive sampling technique. In this study, sample were divided into 2 groups and each consist of 20 people which are handrubbing and handwashing groups. Bacterial samples were obtained by placing the right hand in a container that contain NaCl 0.9% solution for 1 minute. Samples were then grown in nutrient agar plate and incubated at 37°C for 48 hours. The culture results were calculated using the colony counter tool. Results: There is a significant difference before and after handrubbing with significance 0.00 (P<0.05) and before and after handwashing with significance 0.001 (p<0.05) in reducing bacterial colonization in hand with. However, there is no significant association between handrubbing and handwashing method in reducing the number of bacterial colonization with a significance number of 0.892 (p> 0.05). Conclusion: There is no significant differences between handrubbing and handwashing in reducing the number of bacterial colonization.

Objective: To compare post-odontectomy wound healing time using silk and catgut sutures at Pirngadi Hospital from May to June 2018. Material and Methods: This is anon-laboratory experimental study with a "post-test only"design. Wound healing scores was assessed on the first and seventh days post odotectomy by seeing signs of inflammation. The number of patients studied was 30 people which 15 people used silk and 15 people used catgut. Results: The results were statistically analyzed using Mann-Whitney Test. There was no significant relationship (p> 0.05) between the wound healing time to the suture type on the first day post odontectomy. On the seventh day, it was found that there was a significant relationship (p <0.05) which silk had a score of 1.40 ± 0.507 while catgut had a score of 1.07 ± 0.258. Conclusion: Catgut has a better wound healing time than silk.

Material and Methods: This was an experimental study with posttest only control group design approach. Purposive technique is applied to collect samples which are lower molar extraction forceps. In this study, sample were divided into 2 groups and each consisting of 18 instruments. The treatment group was treated with 2% glutaraldehyde while the control group was treated with 4.8% chloroxylenol. Each instrument was pre-cleaned using a brush, water and soap for both groups underwent the disinfection process. Results: The results were statistically analyzed using Mann-Whitney Test. The comparison between glutaraldehyde and chloroxylenol showed a significant difference to the total bacteria count on instrument after disinfection (p=0.014 < 0.05). Conclusion: 2% glutaraldehyde was more effective than 4.8% chloroxylenol at disinfecting lower molar extraction forceps.

Objective: To compare Chloroxylenol 4.8% and Povidone Iodine 7.5% on total bacterial count post WHO routine hand washing on clinical students at the department of oral surgery, Faculty of Dentistry, Universitas Sumatera Utara Material and Methods: This was an experimental study with posttest only control group design approach. Purposive technique is applied to collect samples which are clinical students. In this study, sample were divided into 3 groups and each consisting of 10 people. Aquadest was used as control group while Chloroxylenol 4.8% and Povidone Iodine 7.5% was used as the treatment group. Each member performed routine handwashing using WHO method for 1 minute long. Results: The results were statistically analyzed using Kruskal Wallis and Mann-Whitney Test. The comparison between Chloroxylenol and Povidone Iodine showed no significant difference to the total bacteria count after handwashing (p>0.05) but the Chloroxylenol group showed a greater mean reduction. Conclusion: Chloroxylenol and Povidone Iodine are capable reducing the number of bacteria in hands, therefore it can be a good antiseptic choice for hand washing.

Material and Methods: This is an experimental research with a post-test only approach. The sampling technique used is Purposive Sampling. In this study, sample were divided into 2 groups consisting of 18 extraction instruments which are lower molar forceps rubbed with 70% alcohol and soaked in 70% alcohol. Each instrument were pre-cleaned using brush, water and soap prior disinfection process. Results: The results were statistically analyzed using Mann-Whitney Test. The result showed no significant difference to the total bacteria count on rubbing and soaking using 70% alcohol. Conclusion: Soaking in 70% alcohol method showed more effective because it overally kills bacteria.

Material and Methods:This was an experimental study with post-test only control group design approach. Purposive technique is applied to collect samples which are lower molar extraction forceps. In this study, sample were divided into 2 groups and each consisting of 18instruments soaked in Glutaraldehyde 2% and Hydrogen Peroxide 3%. Each instrumentwere pre-cleaned using brush, water and soap for both group before going through disinfection process. Results: Statistically analyzed using Mann-Whitney Test. The comparison between Glutaraldehyde 2% and Hydrogen Peroxide 3%showedno significant difference to the total bacteria count on instrument after disinfection (p=0.014 <0.05). Conclusion: Glutaraldehyde 2% showed more effective than Hydrogen Peroxide 3% in disinfecting lower molar extraction forceps.

Objective: Disinfection is an act to kill and eliminate all forms of microorganisms including spores with physical or chemical procedures. One of the goals of disinfection in the health sector is to prevent cross infection through microorganisms that are found in blood, saliva and dental plaque that can contaminate the work surface. Material and Methods: This is an experimental research with pretest-posttest design. This study was conducted on 10 dental chair unit and the number of bacterial colonies is observed before and after disinfection using 70% alcohol and 2% glutaraldehyde. Results: The results showed that the number of bacterial colonies experienced a statistically significant decrease after disinfection with 70% alcohol with mean value of 318.20±217.52 before and 11.20±17.29 after disinfection, while 2% glutaraldehyde decreased with mean value before 194.20±206.61 and after disinfection 2.20±2.28. Comparison of the difference in mean number of bacterial colonies between 70% alcohol group and 2% glutaraldehyde group did not show a significant difference (p = 0.412). Conclusion:Based on the results, it can be concluded that there is no significant difference in the number of bacterial colonies between 70% alcohol and 2% glutaraldehyde. From the results, it can be concluded that there was nothing better in the act of disinfection of dental chair units using 70% alcohol and 2% glutaraldehyde in reducing the number of bacteria.

To compare the different amount of bacterial colonies adhered on silk and catgut sutures in odontectomy patient at Dr. Pirngadi Hospital from May to July 2018. Material and Methods: This is laboratory experimental study with a "post-test only control group" design which means the threads that have been remove on 7th day postoperative and would be transferred to Microbiology Laboratory, Faculty of Mathematic and Science, Universitas Sumatera Utara. Bacterial colony counter were used to count the amount of bacteria colony on a petri dish containing PCA medium after the colonization was diluted to 10 -7 mL. The number of patients studied were 26 people, therefore 13 people used silk sutures and 13 people used catgut sutures.was diluted to 10 -7 mL. The number of patients studied were 26 people, therefore 13 people used silk sutures and 13 people used catgut sutures. Results: Statistically analyzed using Mann-Whitney Test. There was no significant difference (p> 0.05) between the amount of bacterial colonies adherence on silk and catgut sutures. The mean bacterial colony on silk sutures are 207.38±173.605 x10 7 CFU/mL, meanwhile on catgut sutures are 115.15±158.905 x10 7 CFU/mL. Conclusion: Catgut suture could minimalize bacterial colonization better than silk suture.