Steviol glycosides contained in Stevia leaves have considerable potential uses for the natural sweetener industry due to it's antidiabetic properties, hence a large number of plant source is required to supply the industry. To overcome the hindrances in the propagation of Stevia plants using in vivo methods, an alternative technique of propagation using in vitro methods is needed. The micropropagation method with the RITA® (Recipient for Automated Temporary Immersion System) is used in the production of a large amount of stevia biomass in approximately a short period. The forming of flowers is one of the limiting factors interfering with the metabolite production, as the content of steviol glycoside will decrease after plant flowering dramatically. This mechanism happened because steviol glycoside synthesis and flowering process share the same precursor. However, this interfering factor could be inhibited by using a high-red LED induction to delay the flowering stage, which may contribute to higher biomass and glycoside concentrations through greater system productivity. Hitherto, there was no significant study about the effect of far-red LED induction to improve the steviol glycoside content of Stevia plants, particularly at the molecular level. This research was therefore performed in the TIS RITA ® bioreactor system to evaluate the effect of far-red LED induction towards biomass growth, multigene expression related to steviol glycosides, and its derivatives, and also the metabolites produced in S. rebaudiana plant. The result showed that the increment of biomass and gene expressions (ent-KO, ent-KS, ent-KAH13, UGT85C2, UGT74G1, and UGT76G1) in high far-red LED RITA ® was higher compared with control RITA ® . To conclude, the system with a far-red LED induction in TIS RITA ® was proven to give a positive effect towards stevia shoots growth and greater metabolites production (stevioside and rebaudioside-A) up to 37.15% and 22.99%, respectively.
Stevia plant contains steviol glycosides, which are estimated to be 300 times sweeter than sucrose. However, conventional (in vivo) propagation of Stevia rebaudiana in Indonesia was not effective due to poor results. Therefore, an alternative method to propagate stevia plants is needed. One of them is by using in vitro method. Multiplication with a large quantity of stevia biomass in a relatively short period can be conducted by using TIS RITA® (Recipient for Automated Temporary Immersion System). This study aimed to evaluate the effect of the immersion period of the medium on growth and the medium bioconversion into the production of shoot biomass. The bioreactors were set up with 15 min and 30 min immersions periods, scheduled every 6 h, and incubated for 21 d. The result indicated that the immersion period affected the biomass and growth rate (µ). Amount of 30 min immersion showed a greater percentage of shoot multiplication, higher biomass, percentage of leaf growth, growth rate, and productivity compared to 15 min immersion. The pattern of sucrose, mineral, and inorganic compounds consumption followed the growth of plant biomass for both systems. In conclusion, 30 min immersion gave a greater efficiency medium bioconversion to plant biomass compared with 15 min immersion.
Sumenep is one of the salt producers in Indonesia. The problem experienced by farmers is the production of salt using evaporation by solar energy that depends on the weather and the low price, caused by worse business management. Salt is a potential commodity, because its market is still wide open. This research aims to develop the salt production method and development strategy of salt business in Sumenep Regency by using SWOT analysis. The result of the analysis concludes that the priority of salt development business strategy in Sumenep Regency are: i) Geoisolator technology application strategy to produce good quality of salt; ii) cooperation strategy in group mechanism to build power and increasing the bargaining value of the farmers; iii) capital strengthening strategy through partner cooperatives; iv) broader marketing management management strategies to industrial salt user sectors.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.