Banyaknya spesies invertebrata laut yang memiliki kemiripan morfologi menyebabkan kesalahan identifikasi sangat mungkin terjadi. Identifikasi spesies secara molekuler sangat diperlukan dalam mempelajari taksonomi yang akurat. Penelitian ini bertujuan untuk menentukan identitas invertebrata laut dari Perairan Papua dengan teknik DNA barcoding menggunakan marka gen COI (Cytochrome c oxidase I). Penelitian mengidentifikasi 29 individu invertebrata laut, yang terdiri dari teripang (6 sampel), lobster (6 sampel), gurita (6 sampel), chiton (5 sampel), dan bulu babi (6 sampel). Metode identifikasi molekuler terdiri dari isolasi DNA, PCR fragmen gen COI, sekuensing, dan analisis sekuens DNA menggunakan software bioinformatika.Hasil penelitian ini menunjukkan bahwa teripang merupakan spesies Bohadschia marmorata, lobser merupakan spesies Panulirus versicolor, gurita merupakan spesies Octopus cyanea, chiton merupakan spesies Ischnochiton australis, dan bulu babi merupakan spesies Tripneustes gratilla, berdasarkan analisis BLAST dan Boldsystem. Dengan tingkat kemiripan sampel dan rujukan dalam kisaran 84.58 sampai 100.00%. Indeks disparitas, jarak genetik dan pohon filogenetik mendukung hasil ini.
SARS-CoV-2 is a new strain of coronavirus (CoV) that was identified in Wuhan in 2019. This virus is known to have the ability to reduce human immunity. Kelor (Moringa oleifera) is a potential natural resource in Indonesia, which is very abundant and contains several metabolic compounds such as phenolics, flavonoids, saponins, cytokines, and caffeoylquinic acid, which was reported to show antioxidants, antibacterial and antiviral. This study aims to predict the biological activity, physicochemical properties, toxicity, and affinity-interactions of the active compounds of M. oleifera leave. The active compounds of M. oleifera were obtained from the KNApSAcK and PubChem. Analysis of the bioactivity of the compounds using the Way2Drug Pass Online. Analysis of drug-likeness and toxicity using the Lipinski web server and pkCSM. Docking is done using Autodock vina software to analyze the interaction of the compounds with Mpro. The results indicate that the compound astragalin is the compound with the highest affinity value, namely -8.7 (kcal/mol), compared to lopinavir as a control compound with an affinity value -6.6 (kcal/mol). The types of bonds in astragalin compounds are hydrogen bonds with amino acids Glutamine 127 and Arginine 298. From these results, it is predicted that astragalin compounds have the highest potential as alternative drugs to increase body immunity against the COVID-19.
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