Research about chromatographic profile of antioxidant and antibacterial compounds in nhexane fraction of Libo leaves (Ficus variegata Blume.)
Key Words: n-hexane fraction of Libo leaves (Ficus variegata Blume.), chromatography, antioxidant, antibacterial
ABSTRAKPenelitian mengenai profil kromatografi senyawa antioksidan dan antibakteri dalam fraksi nheksana dari daun Libo (Ficus variegata Blume.) telah dilakukan dengan tujuan untuk mengetahui profil kromatografi senyawa antioksidan dan antibakteri dalam fraksi n-heksana dari daun Libo. Ekstraksi dilakukan secara maserasi dengan menggunakan pelarut metanol. Isolasi dilakukan dengan 2 metode, kromatografi kolom vakum (KCV) kemudian dilanjutkan dengan kromatografi kolom konvensional (KK). Eluen yang digunakan yaitu n-heksan-etil asetat dan metanol-kloroform. Hasil pemisahan yang diperoleh berdasarkan kromatografi kolom konvensional yaitu sebanyak 10 fraksi. Fraksi-fraksi tersebut kemudian dilakukan pengujian aktivitas antioksidan secara kualitatif menggunakan metode DPPH serta pengujian aktivitas antibakteri menggunakan metode KLT bioautografi. Hasil penelitian yang diperoleh berupa profil kromatografi fraksi yang memiliki aktivitas antioksidan dan antibakteri, yaitu sebanyak 5 fraksi (NHB1, NHB3, NHB4, NHC1, NHC3) aktif sebagai antioksidan dan 4
Dilakukan penelitian dengan judul Analisis Kadar Total Flavonoid dan Uji Aktivitas Antioksidan Ekstrak Daun Kelubut (Passiflora foetida). Penelitian ini bertujuan untuk mengetahui kandungan total flavonoid serta potensi antioksidan ekstrak daun kelubut (Passiflora foetida). Pengujian kandungan total flavonoid menggunakan metode spektrofotometri UV-Vis dengan pereaksi dan alumunium klorida, gambaran hasil flavonoid yaitu menggunakan ekuivalen kuersetin sebagai standar. Sedangkan pengujian aktivitas antioksidan dilakukan dengan menggunakan metode DPPH dan gambaran aktivitas antioksidan yaitu dinyatakan dengan IC50. Hasil yang didapat lalu dihitung dengan menggunakan analisis data regresi linier. Hasil kadar total flavonoid tertingi terdapat pada fraksi etil asetat sebesar 5.44 mg QE/g dan hasil uji aktivitas antioksidan ekstrak daun kelubut (P. foetida) berpotensi sedang yang terdapat pada fraksi etil asetat sebesar 105.840 ppm.
Piper betle var. nigra is a tropical plant closely related to the common piper. P. betle has also been dubbed a promising source of natural antioxidants in herbal health products, antibacterial, antifungal, antimalarial, cytotoxic activity against the cancer cell lines K562 and HL-60, and antileishmanial. The aim of this study to observation Antimicrobial activity and isolation of chemical compound. The antimicrobial activity of P. betle extract was performed by well diffusion method against two oral pathogenic bacteria (Streptococcus mutans and Streptococcus sanguinis) and opportunistic pathogenic yeast (Candida albicans). The inoculum (bacterial and yeast suspension) was prepared from a 24-h culture on NB for bacterial suspension and on TSB for yeast suspension. Extraction and isolation using various method of chromatography. Isolated compounds were characterized by spectroscopic means. Our study showed antimicrobial activity from crude ethanol extract of leaves P. betle L. var. nigra against two oral pathogenic bacteria and opportunistic pathogenic yeast with concentration 0.5% and 1%. The first report of two new amides derivatives, piperenamide A (1) and piperenamide B (2) in P. betle L. var. nigra.
Ficus variegata Blume is specific plant of east Kalimantan. Flavonoid compound of Ficus variegata Blume was isolated by vacuum liquid and column chromatography, with previously extracted by maceration method using n-hexane and methanol, and fractination using ethyl acetate solvent. Eluent used in isolation were n-hexane:ethyl acetate (8:2). The results of elucidation structure by using spectroscopy methods (GC-MS, NMR, and FTIR) was obtained 5-Hidroxy-2-(4-methoxy-phenyl)-8,8-dymethyl-8H-pyrano[2,3-f] chromen-4-one. This compound has an antibacterial and antioxidant activities.
Snakefruit (Salacca zalacca) is one of Indonesian native plant that the pericarp of snakefruit can be used because it has activity as an antimicrobial. This study aims to identify the metabolite content of snakefruit pericarp and to investigate the potential of snakefruit pericarp extracts in inhibiting the growth of Streptococcus mutans and Candida albicans. Snakefruit pericarp was extracted by reflux method using ethanol 96%, and an extract was tested by phytochemical qualitatively and antimicrobial activity of extract was carried out using the well method, by using concentrations are 5, 10, 15, and 20%. The results showed that the extracts contains secondary metabolites i.e. alkaloids, flavonoids, saponins and tannins. Extract of snakefruit pericarp can inhibit the growth of Streptococcus mutans and Candida albicans at 5% concentration and the inhibiting activity increased together with higher concentrations of extract.
Uncaria is a genus of plants that are widely distributed in the tropics. There are about 5 of the 38 species of this genus growing in the tropical rain forests of East Borneo, Indonesia. For a long time, Uncaria is commonly used as a traditional medicine to treat various diseases by the Dayak tribe in Kalimantan, traditional people believe that Uncaria may be cured cancer, tumors, mioms, and cycts. Based on previous studies, the activity of the genus Uncaria has been widely reported such as cytotoxic, antimicrobial, antioxidant, antidiabetic, and thrombolytic activities. This article aims to summarize the potential of the Uncaria genus, focusing on 5 species from East Borneo, namely Uncaria nervosa, Uncaria longiflora, Uncaria gambir, Uncaria tomentosa and Uncaria cordata. The method used in this article is a literature study by collecting previous research articles related to the Uncaria genus. The results of the literature study show that the Uncaria genus in East Borneo has many secondary metabolites with diverse chemical structures that show good biological potential so that they can be used as broad and promising insights for drug discovery and development. This paper is also expected to provide input for the policy of conservation of medicinal plants in the forests of East Borneo.
Kokang (L. amoena (Hassk) Leenh.) merupakan tanaman lokal Kalimantan Timur dan digunakan sebagai alternatif pengobatan oleh suku dayak untuk mengatasi berbagai masalah kulit, diantaranya menghilangkan noda hitam di wajah, menyembuhkan bekas luka cacar, bekas jerawat dan perawatan kulit (skincare). Penelitian ini bertujuan untuk memperoleh isolat senyawa metabolit sekunder yang memiliki aktivitas antioksidan dan mengetahui struktur kimia dari isolat tersebut. Metode yang digunakan adalah ekstraksi secara maserasi dengan metanol. Ekstrak difraksinasi dengan n-heksan, etil asetat dan n-butanol. Fraksi etil asetat diisolasi dengan menggunakan berbagai teknik kromatografi. Pengujian antioksidan menggunakan metode kualitatif dengan DPPH. Hasil isolasi didapatkan isolat EA31 yang memiliki aktivitas antioksidan. Hasil elusidasi struktur isolat berdasarkan MS, 1H-NMR dan 13C-NMR. diperoleh senyawa dengan rumus molekul C15H12O11 yang meberikan aktifitas terdapat radikal bebas DPPH.
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