Novel Amides Derivative with Antimicrobial Activity of Piper betle var. nigra Leaves from Indonesia

Abstract

Piper betle var. nigra is a tropical plant closely related to the common piper. P. betle has also been dubbed a promising source of natural antioxidants in herbal health products, antibacterial, antifungal, antimalarial, cytotoxic activity against the cancer cell lines K562 and HL-60, and antileishmanial. The aim of this study to observation Antimicrobial activity and isolation of chemical compound. The antimicrobial activity of P. betle extract was performed by well diffusion method against two oral pathogenic bacteria (Streptococcus mutans and Streptococcus sanguinis) and opportunistic pathogenic yeast (Candida albicans). The inoculum (bacterial and yeast suspension) was prepared from a 24-h culture on NB for bacterial suspension and on TSB for yeast suspension. Extraction and isolation using various method of chromatography. Isolated compounds were characterized by spectroscopic means. Our study showed antimicrobial activity from crude ethanol extract of leaves P. betle L. var. nigra against two oral pathogenic bacteria and opportunistic pathogenic yeast with concentration 0.5% and 1%. The first report of two new amides derivatives, piperenamide A (1) and piperenamide B (2) in P. betle L. var. nigra.

Evaluation of Anti-Candida Potential of Piper nigrum Extract in Inhibiting Growth, Yeast-Hyphal Transition, Virulent Enzymes, and Biofilm Formation

“…According to recent reports, the ethanolic extract of P. nigrum showed antifungal activity with MIC values between 500–2000 μg/mL against reference strains of C. albicans using the disc diffusion technique [ 48 ]. Our results are comparable with studies that have demonstrated the antibacterial and antifungal activity of the ethanolic extract of P. nigrum against clinically important pathogens such as E. coli, S. aureus, and C. albicans [ 70 , 71 ]. Furthermore, some studies have shown that the ethanol fraction is often the most effective [ 72 , 73 ].…”

Chemical constituents from Piper betle L. Var Nigra (Piperaceae)

“…2-hexenoic acid (2) was observed as an Oil yellow, with its molecular composition established as C6H10O2, based on HR-TOFMS. This showed a [M+H]+ ion peak at m/z 115.1211 (calcd.…”

“…In a previous study of Piper betle L. Var. nigra Two amide derivatives, piperenamide A-B have also been reported to have activity against two oral pathogenic bacteria and opportunistic pathogenic [2].…”

Antifungal and Immunomodulatory Ingredients from Traditional Chinese Medicine

“…nigra is a plant used as a spice and medicine belonging to the Piperaceae family. It contains many bioactive chemicals such as cadinene, caryophyllene, and amide alkaloids [79,80], and the extract of P. betle showed inhibitory effects against C. albicans according to a disk diffusion test [81].…”