of the Essential Oils of Origanum vulgare ssp. vulgare L. and O. vulgare ssp. hirtum (Link) letswaart* 2003, 57, 95-98 K. Veres 1. / E. Varga 1/,~. Dobos ~ / Zs. Hajd0 ~ / I. Mdth6 ~' 3 / Ft. N6meth 2 / K. SummaryThe essential oils of Origanum vulgare L. ssp. hirtum (Link) letswaart and Origanum vulgare L ssp. vulgare (Fam. Lamiaceae), cultivated in Hungary, have been studied byGC and GC-MS and the qualitative and quantitative chemical composition of the essential oils in the f,,vo species have been compared. O. vulgare ssp. hirtum oil was found to contain carvacrol (76.4%), ?terpinene (6.6%), thymol (0.23%), and p-cymene (4.7%) as the main constituents whereas the major compounds in O. vulgare ssp. vulgare oil were p-cymene (22.3%), caryophyllene oxide (10.2%), sabinene (Z9%), 7-terpinene (5.1%), thymol (0.34%), and spathulenol (4.8%). The stability of content and composition of the oils during the flowering period (economically beneficial period) were observed. The effect of long-term storage on the composition of the oil was also investigated for both the crude and distilled oil of Origanum vulgate ssp. vulgate.

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The volatile oil compositions of five Stachys species (Stachys officinalis L., St. grandiflora Host., St. byzantina Koch., St. germanica L. and St. sylvatica L.) have been studied using a GC/MS technique. All species gave low yields of oil. From St. officinalis 38 compounds were separated and partly identified, from St. grandiflora 24, St. byzantina 30, St. germanica 29 and St. sylvatica 43. All the samples contained o-ocymene, β-phellandrene, γ-terpinene, linalool, trans-and cis-pinocamphone, βcaryophyllene, δ-cadinene and α-cadinol. The steam distilled oils were poor in mono-but rich in sesquiterpenes. Some of the sesquiterpenes, like germacrene D, epi-cubebol, and αand γ-muurolene are reported in Stachys species for the first time. The iridoids, harpagide and 8-O-acetyl-harpagide, were identified by TLC in the MeOH extracts of St. sylvatica, St. byzantina, St. officinalis (Betonica officinalis) and Betonica serotina. Taking other chemical constituents also into account Stachys species seem to be similar to those of the subfam. Lamioideae (according to Erdtman's classification).

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Notes(1.5 kg) were extracted with methanol at room temperature by percolation. After removal of the solvent, the extract was dissolved in CHCL3 (1.51) and washed with H20 (11). The CHCI3 phase yielded 4.3 g of extract which was column chromatographed over silica gel (50g) with Cl-Id3 (21) followed by CHCI3-acetone (95 : 5) (2 1),

The volatile constituents of both dried and fresh leaves, twigs and flowers of Philadelphus coronarius L. (Hydrangeaceae) were isolated by hydrodistillation and analyzed by GC/FID and GC/MS. There were major differences between the various oils. For example, epi-13-manool (47.6 %) was the major component of the fresh flower oil, but was not detected in the dried flower oil, whereas isolongifolol (15.5%), 2-nonanol (11.1%) and 7-hydroxycoumarin (9.7%) were the principal components of the dried flower oil. In the oils of both the fresh and dried leaves the major constituent was (E,E)-farnesol, although the proportion of this compound in the fresh leaf oil was considerably higher (37.3%) than that of the dried leaf oil (3.2%). (E,E)-farnesol was also the major component of the fresh twig oil (16.6%), but was absent from the dried twig oil, the major constituent of which was (E,E)-2,4-decadienal (2.4%); however, this was not detected in the fresh twig oil.The plant materials were processed either fresh (collected in June, 2002) or dried at room temperature (collected in June, 2001). The oils were isolated by hydrodistillation for 4 h in a Clevenger-type apparatus, the oils being distilled into n-hexane as an absorbing medium, and dried over anhydrous sodium sulfate (1). The oils were stored in glass bottles at 4°C prior to analysis. Oil yields were 0.06% (V/m), 0.06% (v/m) (fresh and dried flowers oils), 0.05 % (v/m), 0.04 % (v/m) (fresh and dried leaves oils), 0.04 % (v/m), 0.04 % (v/m) (fresh and dried twigs oils), respectively. Previous WorkPhiladelphus coronarius is a popular ornamental shrub (2,3). Aqueous extracts of its flowers are used in traditional medicine and in homeopathy (4,5), but crude aqueous extracts of the leaves have been reported to be cytotoxic and as having antibacterial activities (6,7).Even though this plant has been a favourite in European gardens for centuries, little is known about its chemistry. Umbelliferone, scopoletin, stigmasterol-3β-d-glucoside and sitosterol-3β-d-glucoside were isolated from the chloroform extract of the leaves, and from the light petroleum extract, uvaol and 3β, 28-dehydroxyolean-11(12),13(18)-diene were obtained. The occurence of taraxasterol, β-amyrin, ursolic acid and oleanic acid in the light petroleum extract of the twigs (without leaves) was reported (8-10). The few volatile constituents recorded for P. coronarius to date include nopinone, trans-myrtanol, myrtenal, myrtenol, 2-formamidobenzaldehyde, veratraldehyde, 4,8-dimethylnona-1,3,7-triene and 4,8,12-trimethyltrideca-1,3,7,11-tetraene. The major constituents were Downloaded by [University of Tennessee, Knoxville] at 22:17 26 December 2014 Czigle et al.