LIQUID CHROMATOGRAPHIC MONITORING OF PHYTOECDYSTEROID PRODUCTION OF <i>SERRATULA WOLFFII</i>

Báthori, Mária; Gergely, András; Kalász, Huba; Nagy, Gabe; Dobos, Ágnes; Máthé, Imre

doi:10.1081/jlc-100101452

Cited by 12 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 14a-hydroxy-7-en-6-one chromophore is responsible for ultraviolet (UV) absorption with max at 242 nm in methanol (Dinan 2001). To increase detection specificity for ECD analysis, HPLC combined with UV detection at 254 nm, instead of 242 nm, can be used (Bathori et al 2000).…”

Section: Structure and Types Of Phytoecdysteroidsmentioning

confidence: 99%

Biosynthesis and potential functions of the ecdysteroid 20-hydroxyecdysone — a review

Festucci-Buselli

Contim

Barbosa

et al. 2008

Botany

View full text Add to dashboard Cite

The ecdysteroid 20-hydroxyecdysone (20E) is a steroid hormone found in arthropods and plants. It is suspected to have agrochemical, biotechnological, medicinal, and pharmaceutical applicability. In insects, 20E controls or elicits molting and other developmental processes, and several characterized P450 enzymes are involved in its biosynthesis. In plants, it may act as a defensive substance against insects and nematodes. It is suspected that 20E, being a physiologically active compound, may affect morphological and physiological processes in plants and that C 27 phytosterols may be its precursors. However, neither its precise function nor its mechanism of biosynthesis in plants is fully understood. Here, the importance of 20E and current understanding of its structure, potential functions, and biosynthesis in both plants and insects are reviewed.Résumé : L'ecdysteroïde, le stéroïde hormonal 20-hydroxyecdysone (20E), se retrouve chez les arthropodes et les plantes. On croit qu'il aurait des applications agrochimiques, biotechnologiques, médicinales et pharmaceutiques. Chez les insectes, le 20E contrôle ou induit la mue et autres processus morphogénétiques, et on compte plusieurs enzymes P450 caractérisées impliquées dans sa biosynthèse. Chez les plantes, il peut agir comme substance de défense contre les insectes et les néma-todes. On croit que le 20E, une substance physiologiquement active, pourrait affecter des processus morphologiques et physiologiques chez les plantes et que des phytostérols en C27 pourraient en être les précurseurs. Cependant, on ne comprend pas complètement sa fonction précise ni le mécanisme de sa biosynthèse. Les auteurs passent en revue la compré-hension actuelle de sa structure, de ses fonctions potentielles et de sa biosynthèse, chez les plantes et chez les insectes.

show abstract

Section: Structure and Types Of Phytoecdysteroidsmentioning

confidence: 99%

Biosynthesis and potential functions of the ecdysteroid 20-hydroxyecdysone — a review

Festucci-Buselli

Contim

Barbosa

et al. 2008

Botany

View full text Add to dashboard Cite

show abstract

“…However, a simple clean-up on a polyamide micro-column retains the total amount of 20-hydroxyecdysone (and polypodine B) while the overwhelming majority of the interfering components are removed. LC requires clean-up using both Samplex (BioSeparation Technique, Budapest) and polyamide [11]. Without, and even with, the use Samplex C18, the LC separation of the plant sample exhibited a poorly resolved chromatogram.…”

Section: Discussionmentioning

confidence: 99%

“…Without, and even with, the use Samplex C18, the LC separation of the plant sample exhibited a poorly resolved chromatogram. It was found earlier [11], that fla- Pre-separation purification of the samples on polyamide was used for both TLC and MEKC. Both methods resulted in acceptable separation, although MEKC showed better resolution between polypodine B and 20-hydroxyecdysone.…”

Section: Discussionmentioning

confidence: 99%

Determination of 20-hydroxyecdysone content by thin-layer chromatography and micellar electrokinetic chromatography

Báthori

Máthé

Guttman³

1998

Chromatographia

Self Cite

View full text Add to dashboard Cite

SummarySeasonal dependence of 20-hydroxyecdysone content of Serratula tinctoria and Serratula wolffii (Asteraceae) was investigated by micellar electrokinetic chromatography (MEKC).Samples were collected each month through the vegetation period. The leaves were dried, milled and extracted with methanol. Clean-up of the extracts was by solid-phase extraction using a polyamide microcolumn to remove flavonoids and other plant phenolics which can interfere with the analysis. This work deals with the separation of 20-hydroxyecdysone from polypodine B and the seasonal variation of 20-hydroxyecdysone concentration. Determinations have been performed by both thin-layer chromatography and capillary electrophoresis using micellar electrokinetic chromatography.

show abstract

“…To ensure the proper selectivity, these following mobile phases could be checked: [25]. The method used an automated spot application, developments using the adequate mobile phase, and the ultraviolet absorbance scanning densitometry of fluorescent-quenched spots of samples and that of the standards.…”

Section: Quantification Of Ecdysteroidsmentioning

confidence: 99%

“…Perfect specificity of a definite ecdysteroid determination was given by HPLC/MS or HPLC/MS/MS combination [20][21][22]. HPLC/RIA 10 -9 g [23] Spectrophotometry 10 -6 g [24] TLC 5x10 -5 g [25] groups of ecdysteroids, thereby any hydroxylated compound gave the same reaction), the HPLC/fluorescence detection did not become a popular assay [18][19].…”

Section: Quantification Of Ecdysteroidsmentioning

confidence: 99%

Phytoecdysteroids Effects on Mammalians, Isolation and Analysis

Báthori

2002

MRMC

View full text Add to dashboard Cite

Ecdysteroids are known insect moulting hormones, regulating the insects' metamorphosis. At the same time, ecdysteroids reveal beneficial effects on humans and animals alike. Medicinal plants have been subjected to an intensive research, addressing the presence of ecdysteroids. The possible utilization of medicinal plant deals with their use as raw materials for health preparations and also for the isolation of new phytoecdysteroids. Research on the plant ecdysteroids involves two basic lines. Isolation of major compounds and scout their physiological effects; and isolation of minor ecdysteroids to find new compounds. This review summarizes the recent efforts in the ecdysteroid research including their indication as health improvement preparations, chromatography of ecdysteroids and certain methods for identification of new ecdysteroids.

show abstract

LIQUID CHROMATOGRAPHIC MONITORING OF PHYTOECDYSTEROID PRODUCTION OF SERRATULA WOLFFII

Cited by 12 publications

References 8 publications

Biosynthesis and potential functions of the ecdysteroid 20-hydroxyecdysone — a review

Biosynthesis and potential functions of the ecdysteroid 20-hydroxyecdysone — a review

Determination of 20-hydroxyecdysone content by thin-layer chromatography and micellar electrokinetic chromatography

Phytoecdysteroids Effects on Mammalians, Isolation and Analysis

Contact Info

Product

Resources

About