Antibacterial and antioxidant effects of alcoholic extract for the Cucurbita pepo plant were studied. Properties optical (UV-visible spectrophotometer ) and morphological surface(scanning electron microscopy ) detection of the alcoholic extract was done, and the antibacterial activity was investigated against pathogenic microbes (E.coli, Staphylococcus aureus). Results showed the highest effect for crud extract in the growth of E.coli by the zone of inhibition diameter reached (16.33±0.58 ), followed by Staph. Aureus by the diameter of the inhibition zone reached (12.33±2.30mm). The results indicate that the plant C. pepo can be considered a valuable source of effective antioxidant agents at a 0.8 µg/ml concentration.
Keywords. Cucurbita pepo plant: Alcoholic extract: Antioxidant: Antibacterial Activity
The ethanolic extract leaves of the Cordia myxa plant were used to prepare the biosynthesis of silver nanoparticles (Ag-NPs). The detection of Ag-NPs was done using UV, FTIR, and SEM. We evaluated the anti-bacterial property of ethanolic and nano extract against the growth of two types of pathogenic microbes: Gram -ve bacteria (E. coli) and Gram +ve bacteria (S. aureus). The antibacterial activity of the nano extract was high. In vitro, extracts demonstrated strong antioxidant activity against DPPH at a concentration of 0.8mg/ml. The MTT test was utilized to detect the cytotoxicity of nano extract against lung tumor, and the results indicated that nano extract treatment significantly inhibited the growth of cells (A594 cell line) in a concentration dependent manner. The results indicate, nano extract is a particularly valuable source of antiproliferative and cytotoxic agents.
In this work, antimicrobial potential of silver nanoparticles synthesized from extract of Myrtus communis on different pathogenic bacteria and yeast was investigated. 10 mL of extract was mixed with 90 mL of 2 mM aqueous AgNO3 and heated at 70 °C for 3 min. A change from greenish to dark brown color was observed. Characterization using UV-VIS spectrophotometery and X-ray diffraction analysis were performed. The UV-Vis spectral analysis showed silver surface plasmon resonance band at 425 nm. X-ray diffraction showed that the particles were crystalline in nature with face centered cubic structure (FCC) of the bulk silver with broad beaks at 38.50° and 44.76°. Antimicrobial activity against six microorganisms was tested using well diffusion method. The synthesized silver nanoparticles efficiently inhibited various pathogenic organisms in a dosedependent manner was more pronounced against Gram-positive bacteria than Gram-negative bacteria. The approach of green synthesis seems to be cost effective, eco-friendly and easy alternative to conventional methods of silver nanoparticles synthesis. The powerful bioactivity demonstrated by the synthesized silver nanoparticles leads towards the clinical use as antimicrobial.
The results of the FTIR analysis of the hot aqueous extract of bark indicated that it contains many groups and active compounds, and the results of the bacterial tests conducted by digging and spreading method showed a high inhibitory activity of the hot aqueous extract of cinnamon against all pathogenic bacterial strains and at different concentrations. It was observed that the highest efficacy of the extract was against bacteria an inhibition diameter of 35 mm at concentration 800 mg/mL in Bacillus sp. while the bacterial strains were resistant to most of the antibiotics, in the study (Amoxicillin, Gentamycin, Ampicillin, Erythromycin, Tetracycline) except for gentamicin, which showed inhibition of bacteria Staphylococcus aureus with a diameter 19mm and Serratia sp. in diameter17mm. We conclude from the study that the cinnamon plant contains many active compounds and that the hot aqueous extract of Cinnamomum cassia (cinnamon) bark has a high inhibitory ability for different bacterial strains, which exceeded the inhibitory ability of antibiotics.
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