ABSTRACT:Microalgae are widely used in different industries -pharmaceutical, cosmetic, in those dealing with pollution prevention, aquaculture and especially biofuels. Because of the wide range of use, there is an active interest in microalgae large-scale cultivation. Standard techniques for algae biomass production requires cultivation in nutritious culture media, and well-defined external parameters, such as controlled conditions of light intensity, temperature and aeration gas. Commercial production of microalgae is expensive, and the addition of nutrients in culture media contributes to its high cost. The evaluation of the quality of culture media using wastewater effluents or agro-industrial waste can be a way to reduce algal cultures costs. This research aims to develop a noninvasive method for the testing of effectiveness of agro-industrial waste, previously treated, as culture media through daily monitoring of population increase microalgae. We found that it is possible to detect the growth of the microorganisms by using statistical cumulants as numerical approaches for analyzing the dynamic speckle patterns produced by samples of Chrorella Vulgaris algae cultivated in bold basal culture medium.Key words: Biospleckle, microalgae growing, speckle activity, autocorrelation function, optical density. RESUMEN:Las microalgas, son ampliamente utilizadas en las industrias farmacéutica, cosmética, en aquellas relacionadas con la prevención de contaminación, acuacultura y especialmente en biocombustibles. Debido a su amplio rango de aplicaciones existe un gran interés en el cultivo de microalgas a gran escala. Las técnicas estándar para la producción de biomasa algal requieren de su cultivo en medios nutritivos y parámetros externos bien definidos tales como condiciones controladas de iluminación, temperatura y aireación. La producción comercial de microalgas es costosa y la adición de nutrientes a los medios de cultivos contribuye a aumentar el costo. La evaluación de la calidad de medios de cultivos usando aguas residuales o residuos agroindustriales puede resultar en una vía para reducir costos. Este trabajo tiene por objetivo el desarrollo de un método no invasivo para monitorear la eficiencia de residuos agroindustriales, previamente tratados, como posibles medios de cultivos a través del monitoreo diario del crecimiento poblacional de las microalgas. Hemos encontrado que es posible detectar el crecimiento de los microorganismos usando cumulantes estadísticos como una aproximación numérica para analizar la actividad de patrones de speckle dinámico producidos por muestras de algas Chrorella Vulgaris cultivadas en medio basal Bold.Palabras clave: Biospleckle, crecimiento de microalgas, actividad de speckle, función de autocorrelación, densidad óptica. REFERENCES AND LINKS / REFERENCIAS Y ENLACES[1] H. Rabal, M. Trivi, R. Arizaga, N. Cap, G.Romero, E. Alanís, "Transient phenomena analysis using dynamics speckle patterns," Opt. Eng. 38(1), 57-62 (1996).https://doi.org/10.1117/1.600789[2] G. Romero, E. Alanís, H...
Volcanic ashes features, such as their shapes, textures and sizes, influence their aerodynamic behavior, travel distances, sedimentation and dispersion rates. In particular, their morphology provides information about the fragmentation mechanisms during an eruption, different eruptive styles, magma properties and also is a valuable tool for modeling volcanic plumes. In this work, digital holographic microscopy is used in order to perform a morphological analysis ofashes. A morphological, textural and granulometric 2D and 3D characterization is developed through the calculation of shape and size parameters along with a classification according to the fragmentation mechanism of the eruptive process.
Most digital holographic microscopy architectures introduce a microscope objective in the object arm of the micro-interferometer in order to increase lateral resolution but, as a consequence, a spherical phase aberration arises. The phase distortion must be corrected to achieve reliable phase information linked to the microscopic object under study. In this work, we present a fast and simple numerical method for automatically compensate the phase curvature from the wrapped phase gradient of the reconstructed hologram. Since non-unwrapping methods are required, computational cost is significantly reduced. Furthermore, no additional holograms recording is needed and it does not require prior knowledge of the object or the setup. The method is experimentally demonstrated by a phase contrast imaging of a Pediastrum cell.Key words: phase curvature, digital holographic microscopy, Optical phase aberration, wrapped phase RESUMEN:La mayoría de los montajes experimentales utilizados en microscopía holográfica digital introducen un objetivo de microscopio en el brazo objeto del miro-interferómetro con el objetivo de aumentar la resolución lateral; sin embargo, como consecuencia, aparece una aberración de fase esférica que debe corregirse para acceder al retardo de fase que experimenta es espécimen microscópico bajo estudio. En este trabajo se presenta un método rápido y simple para corregir numéricamente la curvatura de fase a partir del gradiente del mapa de fase envuelto del holograma reconstruido. Dado que no se requiere el empleo de métodos de desenvolvimiento de fase, los costos computacionales son notablemente reducidos. Adicionalmente, no se precisa del registro de hologramas adicionales, ni del conocimiento previo del objeto bajo estudio o del arreglo experimental. El método se demuestra experimentalmente a partir del holograma de una microalga del género Pediastrum.
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