Rolling Shutter Effect aberration compensation in Digital Holographic Microscopy

Monaldi, Andrea Carolina; Romero, Gladis Graciela; Cabrera, Carla; Blanc, Adriana Verónica; Alanís, Elvio

doi:10.1016/j.optcom.2015.12.048

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…A method for correcting this effect was described in Ref. [15]. In order to correct the aberration, the wrapped phase gradient ∇ is computed in Python by means of NumPy, its fundamental package for scientific computing.…”

Section: Resultsmentioning

confidence: 99%

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An automatic wrapped phase gradient-based method for spherical aberration correction in Digital Holographic Microscopy

Monaldi¹,

Romero²,

Blanc³

et al. 2018

Opt. Pura Apl.

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Most digital holographic microscopy architectures introduce a microscope objective in the object arm of the micro-interferometer in order to increase lateral resolution but, as a consequence, a spherical phase aberration arises. The phase distortion must be corrected to achieve reliable phase information linked to the microscopic object under study. In this work, we present a fast and simple numerical method for automatically compensate the phase curvature from the wrapped phase gradient of the reconstructed hologram. Since non-unwrapping methods are required, computational cost is significantly reduced. Furthermore, no additional holograms recording is needed and it does not require prior knowledge of the object or the setup. The method is experimentally demonstrated by a phase contrast imaging of a Pediastrum cell.Key words: phase curvature, digital holographic microscopy, Optical phase aberration, wrapped phase RESUMEN:La mayoría de los montajes experimentales utilizados en microscopía holográfica digital introducen un objetivo de microscopio en el brazo objeto del miro-interferómetro con el objetivo de aumentar la resolución lateral; sin embargo, como consecuencia, aparece una aberración de fase esférica que debe corregirse para acceder al retardo de fase que experimenta es espécimen microscópico bajo estudio. En este trabajo se presenta un método rápido y simple para corregir numéricamente la curvatura de fase a partir del gradiente del mapa de fase envuelto del holograma reconstruido. Dado que no se requiere el empleo de métodos de desenvolvimiento de fase, los costos computacionales son notablemente reducidos. Adicionalmente, no se precisa del registro de hologramas adicionales, ni del conocimiento previo del objeto bajo estudio o del arreglo experimental. El método se demuestra experimentalmente a partir del holograma de una microalga del género Pediastrum.

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Section: Resultsmentioning

confidence: 99%

“…5 a). As can be seen, CMOS aberration may strongly affect estimation; therefore, results could be notably improved by correcting this effect [15] or by using a CCD sensor or a CMOS with global shutter as recording devices. The paraboloid of Eq.…”

Section: Resultsmentioning

confidence: 99%

An automatic wrapped phase gradient-based method for spherical aberration correction in Digital Holographic Microscopy

Monaldi¹,

Romero²,

Blanc³

et al. 2018

Opt. Pura Apl.

View full text Add to dashboard Cite

show abstract

“…To obtain continuous phase map across the windows, a simple phase unwrapping operation [39] may be applied. Further, the phase aberration induced by the rolling shutter effect of the CMOS sensor of the camera is removed using a median filter operation [40]. For experiments and noise characterization of the system, we used the TGXYZ02 test target from Mikromasch.…”

Section: Experimental Setup and Hologram Processingmentioning

confidence: 99%

Nanoscale surface profile measurement using state space approach in digital holographic microscopy

Pandey

Gannavarpu

2023

Surf. Topogr.: Metrol. Prop.

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Surface profile measurement at the nanoscale level has important applications in non-destructive testing and evaluation. The paper proposes a robust method for surface profilometry using digital holographic microscopy setup. The proposed method relies on extracting the phase map encoded in the hologram signal, which directly corresponds to the surface profile, using state space approach. The main advantage of the proposed method is high robustness against noise, which is demonstrated using numerical simulations. For designing the experimental system, a combination of Raspberry-Pi computer and camera module is used for hologram acquisition and processing, which is a step towards low cost imaging. For surface profile measurement, the experimental system has both temporal and spatial sensitivity parameters within 5 nanometers, which indicates robust design. In addition, experimental results show that the proposed method shows superior performance compared to the existing methods for measuring 100 nanometers surface profile features corresponding to different micro-structure regions of a standard calibration test target. Overall, the proposed method allows for single shot, non-contact and full-field measurement of nanoscale surface profile with additional benefits of noise robustness and low cost imaging design.

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“…In obtaining the phase information, there are several problems, such as speckle [6,7] or shot noise [8]. Another effect, a rolling shutter aberration caused by the sequential readout nature of some detectors, has also been studied, and a method for compensating this effect in an image plane DHM has been proposed [9]. In temporal phase-shifting DHM, it was demonstrated that a partially coherent light source (LED) yields a lower phase noise than a coherent one (laser) [10].…”

Section: Introductionmentioning

confidence: 99%

Linearity and Optimum-Sampling in Photon-Counting Digital Holographic Microscopy

et al. 2022

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In the image plane configurations frequently used in digital holographic microscopy (DHM) systems, interference patterns are captured by a photo-sensitive array detector located at the image plane of an input object. The object information in these patterns is localized and thus extremely sensitive to phase errors caused by nonlinear hologram recordings (grating profiles are either square or saturated sinusoidal) or inadequate sampling regarding the information coverage (undersampled around the Nyquist frequency or arbitrarily oversampled). Here, we propose a solution for both hologram recording problems through implementing a photon-counting detector (PCD) mounted on a motorized XY translation stage. In such a way, inherently linear (because of a wide dynamic range of PCD) and optimum sampled (due to adjustable steps) digital holograms in the image plane configuration are recorded. Optimum sampling is estimated based on numerical analysis. The validity of the proposed approach is confirmed experimentally.

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Rolling Shutter Effect aberration compensation in Digital Holographic Microscopy

Cited by 8 publications

References 11 publications

An automatic wrapped phase gradient-based method for spherical aberration correction in Digital Holographic Microscopy

An automatic wrapped phase gradient-based method for spherical aberration correction in Digital Holographic Microscopy

Nanoscale surface profile measurement using state space approach in digital holographic microscopy

Linearity and Optimum-Sampling in Photon-Counting Digital Holographic Microscopy

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