Atherosclerosis is a cardiovascular disease associated with abnormalities of vascular functions. The consumption of mono- and polyunsaturated fatty acids can be considered a strategy to reduce clinical events related to atherosclerosis. In the present study, we investigated the effects of supplementation with 310 mg of ω-3 PUFAs (2:1 eicosapentaenoic/docosahexaenoic acids) for 56 days on rats with hypercholesterolemia induced by a diet containing cholesterol (0.1%), cholic acid (0.5%), and egg yolk. Serum biochemical parameters were determined by the enzymatic colorimetric method. Assessment of vascular effects was performed by analysis of histological sections of the heart and aortic arch stained with hematoxylin and eosin and vascular reactivity of the aorta artery. We observed that treatment with ω-3 PUFAs did not promote alterations in lipid profile. On the other hand, we documented a favorable reduction in liver biomarkers, as well as contributions to the preservation of heart and aortic arch morphologies. Interestingly, the vascular reactivity of rat thoracic aortic preparations was improved after treatment with ω-3 PUFAs, with a decrease in hyperreactivity to phenylephrine and increased vasorelaxation promoted by acetylcholine. Our findings suggest that the supplementation of hypercholesterolemic rats with ω-3 PUFAs promoted improvement in liver and vascular endothelial function as well as preserving heart and aortic tissue, reinforcing the early health benefits of ω-3 PUFAs in the development of atherosclerotic plaque and further related events.
Objetivo: Avaliar se a concentração de Interleucina-6 (IL-6) pode ser utilizada para o monitoramento da resposta ao tratamento com Tocilizumabe (TCZ) em pacientes com artrite reumatoide. Métodos: Trata-se de uma revisão integrativa sustentada em uma pergunta norteadora. Apenas estudos em humanos tratados com TCZ e que apresentavam a concentração de IL-6 foram incluídos. As bases de dados utilizadas foram PubMed, Scientific Electronic Library Online (SciELO) e Biblioteca virtual em saúde (BVS). Resultados: Foram encontrados 257 artigos, no entanto após aplicação dos critérios de inclusão e exclusão apenas 7 artigos foram considerados resultado da pesquisa. A análise dos artigos indica que há uma elevação da concentração de IL-6 após o início da terapia, seguida de um platô e, posteriomente, diminuição com o tempo de tratamento, que foi acompanhada de melhora clínica. Considerações finais: Esse fato sugere a mensuração de IL-6 como um parâmetro importante para monitorar a resposta terapêutica. Para isso, o ideal seria realizar a dosagem de IL-6 antes, durante e após a suspensão do tratamento nos pacientes com remissão da doença. Apenas através de pesquisas será possível padronizar a utilização da IL-6 nesses pacientes.
Mimosa caesalpiniifolia (Fabaceae) is used by Brazilian people to treat hypertension, bronchitis, and skin infections. Herein, we evaluated the antiproliferative action of the dichloromethane fraction from M. caesalpiniifolia (DFMC) stem bark on murine tumor cells and the in vivo toxicogenetic profile. Initially, the cytotoxic activity of DFMC on primary cultures of Sarcoma 180 (S180) cells by Alamar Blue, trypan, and cytokinesis block micronucleus (CBMN) assays was assessed after 72 h of exposure, followed by the treatment of S180-bearing Swiss mice for 7 days, physiological investigations, and DNA/chromosomal damage. DFMC and betulinic acid revealed similar in vitro antiproliferative action on S180 cells and induced a reduction in viable cells, induced a reduction in viable cells and caused the emergence of bridges, buds, and morphological features of apoptosis and necrosis. S180-transplanted mice treated with DFMC (50 and 100 mg/kg/day), a betulinic acid-rich dichloromethane, showed for the first time in vivo tumor growth reduction (64.8 and 80.0%) and poorer peri- and intratumor quantities of vessels. Such antiproliferative action was associated with detectible side effects (loss of weight, reduction of spleen, lymphocytopenia, and neutrophilia and increasing of GOT and micronucleus in bone marrow), but preclinical general anticancer properties of the DFMC were not threatened by toxicological effects, and these biomedical discoveries validate the ethnopharmacological reputation of Mimosa species as emerging phytotherapy sources of lead molecules.
Background:
The search for novel metallic chemical compounds with toxicogenic effects have been of great
importance for more efficient cancer treatment.
Objective:
The study evaluated the cytotoxic, genotoxic and mutagenic activity of organoteluran RF07 in S-180 cell line.
Methods:
The bioassays used were cell viability with 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide
(MTT) test, evaluation of apoptosis and necrosis using fluorescence and flow cytometry, cytokinesis-block micronucleus
test and comet assay. The compound was tested at 1; 2.5 and 5 µM.
Results:
The results showed the cytotoxicity of RF07 at concentrations of 2.5, 5, 10 and 20 µM when compared to the
negative control. For genotoxicity tests, RF07 showed effects in all concentrations assessed by increased index and
frequencies of damage and mutagenic alterations. The compound was also cytotoxic due to the significant decrease in
nuclear division index, with significant values of apoptosis and necrosis. The results of fluorescence and flow cytometry
showed apoptosis as the main type of cell death caused by RF07 at 5 µM, which is thought to avoid an aggressive immune
response of the organism.
Conclusion:
In addition to cytotoxic and genotoxic effects, RF07 creates good perspectives for future antitumor
formulations.
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