In this research, antioxidant activity, total phenolic, and flavonoid content of n-hexane, ethyl acetate, and methanol fractions of roots, stem bark, and leaves of Elaeocarpus mastersii King were determined by the colorimetric method using DPPH assay (1,1-diphenyl-2-picrylhydrazyl), Folin-Ciocalteu reagent, and aluminum chloride reagent, respectively. Phytochemical screening was evaluated by specific reaction of constituents to the specific reagent. Ethyl acetate fraction had the best value of phenolic content and antioxidant activity among all fractions which the best value was obtained from the leaves (380.99 ± 2.14 mg GAE/g DW and IC50 1.95 ± 0.01 µg/mL) followed by the roots (362.88 ± 1.89 mg GAE/g DW and IC50 2.05 ± 0.01 µg/mL) and the stem bark (341.89 ± 3.97 mg GAE/g DW and IC50 2.36 ± 0.02 µg/mL). All fractions of this plant showed the low value of flavonoid content. The phytochemical screening exhibited that this plant was dominated by phenolic and alkaloid compounds. These results demonstrate the great potential of Elaeocarpus mastersii King as a natural antioxidant and active compounds.

No abstract

The anthocyanins in Ficus padana were extracted and identified by using high-performance liquid chromatography/diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI-MS Keywords: Ficus padana; anthocyanin; HPLC-DAD-ESI-MS; antioxidant activity ABSTRAK Senyawa antosianin di dalam buah Ficus padana diekstraksi dan diidentifikasi dengan menggunakan kromatrografi cair kinerja tinggi diode array detektor dan spektrometer massa/elektron spray ionisasi (KCKT-DAD-ESI-SM). Antosianin dideteksi dengan membandingkan antara data spektral masa antosianin yang di dapat dengan data yang sudah dipublikasi. Senyawa pertama (puncak 1) diidentifikasi sebagai pelargonidin [3][4][5][6]

Paracetamol is generally used as analgesic and antipyretic drugs. Contamination paracetamol in the environment can occur because of waste material disposal from production site and immediate disposal of household that cause water pollution. Paracetamol is degraded by photolysis method under irradiation 10 watt UV-light (λ=365 nm), visible-light (Philips LED 13 watt 1400 lux) and solar-light with and without addition C-N-codoped TiO 2 catalyst. The solution is analyzed by UV-Vis spectrophotometer at λ 200-400 nm. Optimum weight of C-N-codoped TiO 2 catalyst obtained is 20 mg under UV-light photolysis. Paracetamol 4 mg/L is degraded 45.48% after 120 minutes under UV-light irradiation without catalyst, and increases to be 69.31% by using 20 mg catalyst. While degradation percentage of paracetamol is 16.96 % without catalyst, the percentage increases to be 34.29% after using 20 mg catalyst for 120 minutes photolysis under visible-light. Degradation of paracetamol by solar light achieves only 12.27% in absance of catalyst for 120 minutes irradiation, but it increases significantly until 70.39% in presence of 20 mg catalyst.

Salix Tetrasperma Roxb. is a plant that found in Indonesia were used as traditional medicine such as diabetes and wound healing. In this study, a flavonoid compound of the ethyl acetate extract of Salix tetrasperma Roxb. leaves was isolated by chromatography technique and the antioxidant activity was determined by DPPH assay. The isolation led to obtain 5,7-dihydroxy-3'-methoxyflavone based on NMR spectra. The ethyl acetate extract exhibited the highest antioxidant activity with the IC 50 is 65.89 µg/mL. This study shows that the Salix tetrasperma Roxb. has good potential as source of antioxidant agent.