Clerodane Diterpenes Isolated from Polyalthia longifolia Induce Apoptosis in Human Leukemia HL-60 Cells

Sari, Dina Permata; Ninomiya, Masayuki; Efdi, Mai; Santoni, Adlis; Ibrahim, S. A.; Tanaka, Kaori; Koketsu, Mamoru

doi:10.5650/jos.62.843

Cited by 16 publications

(12 citation statements)

References 16 publications

(14 reference statements)

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“…Diterpenoids in the hexane extract of P. longifolia seeds has exhibited significant anti-bacterial and anti-fungal activity [ 97 ]. Clerodane diterpenes can induce apoptosis in human leukemia HL-60 cells [ 98 ]. Moreover, 16-Hydroxycleroda-3,13-dien-15,16-olide (HCD) and its analogs extracted from the bark of P. longifolia have strong anti-inflammatory activity [ 99 ].…”

Section: Underlying Mechanisms Of Natural Compound-induced Autophamentioning

confidence: 99%

Natural Compounds from Herbs that can Potentially Execute as Autophagy Inducers for Cancer Therapy

Lin

Tsai

et al. 2017

IJMS

126

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Accumulated evidence indicates that autophagy is a response of cancer cells to various anti-cancer therapies. Autophagy is designated as programmed cell death type II, and is characterized by the formation of autophagic vacuoles in the cytoplasm. Numerous herbs, including Chinese herbs, have been applied to cancer treatments as complementary and alternative medicines, supplements, or nutraceuticals to dampen the side or adverse effects of chemotherapy drugs. Moreover, the tumor suppressive actions of herbs and natural products induced autophagy that may lead to cell senescence, increase apoptosis-independent cell death or complement apoptotic processes. Hereby, the underlying mechanisms of natural autophagy inducers are cautiously reviewed in this article. Additionally, three natural compounds-curcumin, 16-hydroxycleroda-3,13-dien-15,16-olide, and prodigiosin-are presented as candidates for autophagy inducers that can trigger cell death in a supplement or alternative medicine for cancer therapy. Despite recent advancements in therapeutic drugs or agents of natural products in several cancers, it warrants further investigation in preclinical and clinical studies.

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Section: Underlying Mechanisms Of Natural Compound-induced Autophamentioning

confidence: 99%

Natural Compounds from Herbs that can Potentially Execute as Autophagy Inducers for Cancer Therapy

Lin

Tsai

et al. 2017

IJMS

126

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“…Diterpenoids in the hexane extract of P. longifolia seeds shows significant anti-bacterial and anti-fungal activities [ 7 ]. Recently, clerodane diterpenes can induce apoptosis of human leukemia HL-60 cells [ 8 ]. 16-Hydroxycleroda-3,13-dien-15,16-olide (HCD) and its analogs, extracted from the bark of P. longifolia exhibits strong anti-inflammatory activities [ 9 ]; enhanced the expression of cyto-protective HO-1 factor and anti-inflammatory enzyme in microglia [ 10 ]; the induction of apoptosis in leukemia K562 cells via both a reduction in histone modifying enzymes PRC2-mediated gene silencing and the reactivation of downstream tumor suppressor gene expressions [ 11 ] and via PI3K-Akt pathway and Aurora B resulting in gene silencing and cell cycle disturbance [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

The autophagic inhibition oral squamous cell carcinoma cancer growth of 16-hydroxy-cleroda-3,14-dine-15,16-olide

et al. 2017

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16-hydroxycleroda-3, 13-dine-15, 16-olide (HCD) isolated from Polyalthia longifolia possesses numerous biological activities. Previous studies have reported that HCD can block phosphorylation activity of cancer cells to inhibit tumor cell growth, but the anti-tumor activity in oral squamous cell carcinoma is unrevealed. This study investigates the inhibiting effect of HCD on human OSCC cell growth; thereby, developing a new oral cancer drug. In in vitro cultured human OSCC cells (OECM1 and SAS) were employed to test the inhibitory growth of HCD via cell cytotoxic effect using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, Western blotting, and further determining of the inhibitory efficacy of tumor growth by a xenograft tumor on BALB/c male nude mice (in vivo test). Under various concentrations of HCD and time course treatments were shown to effectively cause cell death and cell-cycle arrest in OECM1 and SAS cells, which was confirmed via a clinical drug (cisplatin) as a positive control. In addition, HCD induced the autophagic cell death in OECM1 and SAS cells by LC3-mediated LC3-I/LC3-II/p62 pathway at the in vitro level. An in vivo assay indicated that HCD could treat oral cancer by deferring tumor growth. These findings provide a favorable assessment for further elucidating the role of HCD that targets autophagic cell death pathways as a potential agent for cancer therapy.

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“…Our results showed that 16α-hydroxycleroda-3,13 14 -Z-dien-15,16-olide 3 had the highest activity when compared with polyalthialdoic acid 4 and kolavenic acid 5 18 . It was further noted that the chloroform extract of P. longifolia leaves induced apoptosis through mitochondrial dependent pathway in HL-60 cells 19 .…”

Section: Antileukemic Assaymentioning

confidence: 61%

Comparative Antileukemic Activity of a Tetranorditerpene Isolated from <i>Polyalthia longifolia</i> Leaves and the Derivative against Human Leukemia HL-60 Cells

et al. 2017

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Clerodane Diterpenes Isolated from Polyalthia longifolia Induce Apoptosis in Human Leukemia HL-60 Cells

Cited by 16 publications

References 16 publications

Natural Compounds from Herbs that can Potentially Execute as Autophagy Inducers for Cancer Therapy

Natural Compounds from Herbs that can Potentially Execute as Autophagy Inducers for Cancer Therapy

The autophagic inhibition oral squamous cell carcinoma cancer growth of 16-hydroxy-cleroda-3,14-dine-15,16-olide

Comparative Antileukemic Activity of a Tetranorditerpene Isolated from <i>Polyalthia longifolia</i> Leaves and the Derivative against Human Leukemia HL-60 Cells

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