Comparative Antileukemic Activity of a Tetranorditerpene Isolated from &lt;i&gt;Polyalthia longifolia&lt;/i&gt; Leaves and the Derivative against Human Leukemia HL-60 Cells

Afolabi, Saheed O.; Olorundare, Olufunke E.; Ninomiya, Masayuki; Babatunde, Abiola S.; Mukhtar, Hasan; Koketsu, Mamoru

doi:10.5650/jos.ess17042

Cited by 5 publications

(7 citation statements)

References 18 publications

(13 reference statements)

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“…We had previously reported the purification and isolation of a rare tetranorditerpene from MEP that we characterized using NMR spectroscopic analysis (Figure 8(a)). The compound was confirmed to be a tetranorditerpene 1-naphthalene acetic-7-oxo-1,2,3,4,4a,7,8,8a-octahydro-1,2,4a,5-tetramethyl acid [29]. To ascertain if this is the major active cytotoxic principle from the extract, we performed MTT assay on PCa cells treated with the isolated compound.…”

Section: Resultsmentioning

confidence: 99%

“…We recently isolated active principles from the plant including clerodane diterpene, tetranorditerpene, and other derivatives. We further showed that both tetranorditerpene and clerodane diterpene exerted cytotoxic effect on HL-60 leukemic cell lines [28, 29]. Clerodane diterpene possessed drug resistance modifying potential against methicillin-resistant strain of Staphylococcus aureus [39].…”

Section: Discussionmentioning

confidence: 99%

“…However, it was observed that many of these isolated compounds do not have activity comparable with the plant extracts from which they were isolated [48]. We had previously reported the isolation of a rare tetranorditerpene from MEP and showed its effects on human leukemic HL-60 cells [29]. To determine if this compound was primarily responsible for the growth inhibitory effect of MEP, we evaluated its effect on PCa cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, compounds including cycloartane, triterpenes, clerodane diterpene, tetranorditerpene, and methyl-tetranorditerpene isolated from plant leaves displayed marked growth inhibitory activity in in vitro studies against cancer cell lines [27, 28]. We showed previously that the leaf extract was effective against human leukemia cell lines [29]. Recent findings showed that Polyalthia longifolia induced apoptosis in cervical cancer HeLa cells via the regulation of miRNA, works synergistically with ampicillin against Methicillin-Resistant Staphylococcus Aureus (MRSA) and possesses antiplasmodial activity against chloroquine-sensitive malaria parasite strain NF54 with minimal toxicity to human red blood cells [30–32].…”

Section: Introductionmentioning

confidence: 99%

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Polyalthia longifolia Extract Triggers ER Stress in Prostate Cancer Cells Concomitant with Induction of Apoptosis: Insights from In Vitro and In Vivo Studies

Afolabi

Olorundare

Babatunde

et al. 2019

Oxidative Medicine and Cellular Longevity

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Plant-based therapies are being explored to prevent or treat several cancer types. The antioxidant properties of Polyalthia longifolia plant are well established. In our previous work, we demonstrated the presence of cytotoxic compounds in the methanol extract of Polyalthia longifolia (MEP) with potent activity against human leukemia cells. In the present study, we evaluated the efficacy of MEP against prostate cancer (PCa) and established the molecular basis of its effect in in vitro and in vivo models. We observed that MEP treatment resulted in a significant decrease in the growth and viability of PCa cells, associated with arrest in the G1/S phase of the cell cycle. Apoptosis was confirmed as the primary mode of MEP-induced cell death through activation of the intrinsic apoptotic machinery. Proteomic and biochemical studies identified BiP as an important target of MEP with the activation of the ER stress pathway, as a potential mechanism driving MEP-induced apoptosis. The extract exhibited strong efficacy in the PCa xenograft mouse model with significant inhibition of tumor growth and reduced tumor burden. Taken together, our findings indicate that MEP-induced apoptosis in PCa cells concomitant with the activation of the ER stress pathways results in the inhibition of tumor growth, in vitro and in vivo. Our studies provide initial evidence of the efficacy of MEP against PCa and advocate for in-depth studies in other preclinical models for its possible use in clinical settings.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polyalthia longifolia Extract Triggers ER Stress in Prostate Cancer Cells Concomitant with Induction of Apoptosis: Insights from In Vitro and In Vivo Studies

Afolabi

Olorundare

Babatunde

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Cell proliferation of P3HR1 and K-562 was inhibited by ursolic acid with IC 50 of 2.5 and 17.8 µg/ml. Afolabi et al (2017) isolated a tetranorditerpene from Polyalthia longifolia to treat human leukemic-60 cells after methyl esteri ication of the compound. After treatment, changes in the cellular morphology as indicated by cell shrinkage, blebbing and formation of apoptotic bodies were observed.…”

Section: Cytotoxicitymentioning

confidence: 99%

Targets and Mechanism of Action of Chemical constituents from Plants with Potential Anti-leukemic Activity

Das¹,

M.L²,

Sibi³

2020

ijrps

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The plant kingdom has been the most significant source of anticancer drugs. These include alkaloids, diterpenes, tannins, phenolics, lignans, glycosides which have exhibited lesser toxicity than conventional drugs. In leukaemia, the human body is susceptible to infections due to the replacement of normal leucocytes replaced by a large number of immature cells. Chemotherapy for leukaemia is associated with side effects and drug resistance by the leukemic cells. Cytotoxic agents with higher efficacy and lesser side effects are good candidates in cancer therapy, and plant metabolites serve as potential bioactive agents in anticancer drug formulations. This review article discusses the anti-leukemic properties of compounds obtained from plants and the mechanism of anti-leukemic activity induced by each of these plants. Effect of plants and their metabolites on different leukemic cell lines such as HL60, Kasumi-1, CCRF-CEM, K-562, U-937, THP-1 and MOLT-3 was compared. The findings showed anti-leukemic activity through cell cycle arrest, DNA damage, destruction of mitochondrial function, suppression of tumour genes, apoptosis-inducing enzymes and cytotoxic activities of plants and their derivatives. Based on extensive research ﬁndings from this review, phytochemicals and their derived analogues possess the most promising option for the better and less toxic anti-leukemic treatment. Identification of the mechanism of action by the plant bioactive compounds helps in developing standard herbal medicines for effective leukaemia treatment.

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Phytochemicals from Polyalthia Species: Potential and Implication on Anti-Oxidant, Anti-Inflammatory, Anti-Cancer, and Chemoprevention Activities

2021

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Polyalthia belong to the Annonaceae family and are a type of evergreen tree distributed across many tropical and subtropical regions. Polyalthia species have been used long term as indigenous medicine to treat certain diseases, including fever, diabetes, infection, digestive disease, etc. Recent studies have demonstrated that not only crude extracts but also the isolated pure compounds exhibit various pharmacological activities, such as anti-oxidant, anti-microbial, anti-tumor, anti-cancer, etc. It is known that the initiation of cancer usually takes several years and is related to unhealthy lifestyle, as well as dietary and environmental factors, such as stress, toxins and smoking. In fact, natural or synthetic substances have been used as cancer chemoprevention to delay, impede, or even stop cancer growing. This review is an attempt to collect current available phytochemicals from Polyalthia species, which exhibit anti-cancer potentials for chemoprevention purposes, providing directions for further research on the interesting agents and possible clinical applications.

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Comparative Antileukemic Activity of a Tetranorditerpene Isolated from <i>Polyalthia longifolia</i> Leaves and the Derivative against Human Leukemia HL-60 Cells

Cited by 5 publications

References 18 publications

Polyalthia longifolia Extract Triggers ER Stress in Prostate Cancer Cells Concomitant with Induction of Apoptosis: Insights from In Vitro and In Vivo Studies

Polyalthia longifolia Extract Triggers ER Stress in Prostate Cancer Cells Concomitant with Induction of Apoptosis: Insights from In Vitro and In Vivo Studies

Targets and Mechanism of Action of Chemical constituents from Plants with Potential Anti-leukemic Activity

Phytochemicals from Polyalthia Species: Potential and Implication on Anti-Oxidant, Anti-Inflammatory, Anti-Cancer, and Chemoprevention Activities

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