The present work is focused on fabrication of novel nanofiber (NF) mat as wound-healing scaffold using blends of novel combination of
Hibiscus rosa-sinensis
leaves mucilage (HLM)–Polyvinyl alcohol (PVA)–Pectin,
which was never reported previously. Different ratios of the polymeric blends were electrospun by setting different parameters to achieve best possible electrospun nanofiber mat which was later crosslinked by glutaraldehyde vapor. The optimized formulation of nanofiber mat was characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The crosslinked sample was evaluated for its efficacy in wound healing using Swiss albino mice model, where rapid healing of excised wound was observed with faster epithelization in test mice group than control mice within a period of 8 days. The hemolysis test with optimized crosslinked nanofiber mat CrNF(S7-CL) indicated it to be hemo-compatible. There were no traces of optimized CrNF(S7-CL) when placed under the skin hypodermis in test mice groups revealing its biodegradable nature. The degradation pattern of CrNF(S7-CL) in soil reflects its eco-friendly behavior. Thus, the prepared nanofiber grade CrNF(S7-CL) can be considered as a novel material for faster wound healing and can also be explored for other biomedical applications.
Background: Natural polysaccharides are mostly not stable in their original form but their biodegradable properties can be beneficial for use as polymeric scaffolds for tissue engineering. Microwave irradiation assisted grafting synthesis being an easy and quick method enhances stability of these natural polysaccharides. Materials and Methods: Initially the optimization of the redox initiator ammonium per sulfate (APS) along with the monomer concentration acrylamide (AM) was done by % grafting efficiency (%GE) for getting the optimized grade of graft copolymer (GA-g-PAM). The microwave time was kept constant with the concentration of the polymer Gum acacia (GA). Different analytical techniques were used for the characterization of optimized grade G6 like Fourier transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC), X-ray diffraction(XRD) and Nuclear magnetic resonance (NMR) which confirmed successful grafting reactions and scanning electron microscopy (SEM) was utilized to analyze the interior architecture as well the cell proliferation on to the polymer surface. The optimized grade G6 was inserted under the skin hypodermis for tissue proliferation. Results: The % grafting efficiency (%GE) of optimized grade of Polyacrylamide grafted Gum acacia (GA) graft copolymer (GA-g-PAM) was 94.54%. Histolology studies of local tissue of the test mice revealed that the polymer material after insertion onto the mice skin enhanced the cell proliferation as there were evidences of more collagen as well as fibroblast growth in test animal local tissue than in comparison to control mice. Conclusion: Thus, results indicate that polyacrylamide grafted gum acacia graft copolymer (GA-g-PAM) is sufficiently biocompatible and suitable as versatile material for tissue engineering purpose.
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