Green nanofiber mat from HLM–PVA–Pectin (Hibiscus leaves mucilage–polyvinyl alcohol–pectin) polymeric blend using electrospinning technique as a novel material in wound-healing process

Abstract

The present work is focused on fabrication of novel nanofiber (NF) mat as wound-healing scaffold using blends of novel combination of Hibiscus rosa-sinensis leaves mucilage (HLM)–Polyvinyl alcohol (PVA)–Pectin, which was never reported previously. Different ratios of the polymeric blends were electrospun by setting different parameters to achieve best possible electrospun nanofiber mat which was later crosslinked by glutaraldehyde vapor. The optimized formulation of nanofiber mat was characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The crosslinked sample was evaluated for its efficacy in wound healing using Swiss albino mice model, where rapid healing of excised wound was observed with faster epithelization in test mice group than control mice within a period of 8 days. The hemolysis test with optimized crosslinked nanofiber mat CrNF(S7-CL) indicated it to be hemo-compatible. There were no traces of optimized CrNF(S7-CL) when placed under the skin hypodermis in test mice groups revealing its biodegradable nature. The degradation pattern of CrNF(S7-CL) in soil reflects its eco-friendly behavior. Thus, the prepared nanofiber grade CrNF(S7-CL) can be considered as a novel material for faster wound healing and can also be explored for other biomedical applications.

Electrospun Materials Based on Polymer and Biopolymer Blends—A Review

“…There is also a report on other blends for electrospinning. Sen et al [ 88 ] prepared a blend of two natural polymers (hibiscus leaves mucilage and pectin) and a synthetic polymer (polyvinyl alcohol) by electrospinning technique for the wound healing process. It was found that hibiscus leaves, mucilage, and pectin in nanofiber scaffolds have been effective for wound treatment.…”

“…Electrospinning and film casting methods are used for poly- L -lactic acid (PLA) and chitosan blends [ 88 , 94 ]. PLA/chitosan blend acts as wound dressing material, and it can form an effective barrier against bacteria [ 95 ].…”

From Plants to Wound Dressing and Transdermal Delivery of Bioactive Compounds

“…An interesting valorization of a plant mucilage’s properties (especially charge density here) involves electrospinning, a modern technique applied to obtain nanofibers. Nanofibrillated mats and patches are considered ideal wound healing materials, allowing a sufficient oxygen supply and facile incorporation of the API [ 37 ]. A Plantago ovata mucilage was used as a precursor for the preparation of core–shell nanofibers.…”

Synergistic Effect of Leaf-Pectin from Anisochilus carnosus (L.f.) Wall, Citric Acid, and Lysine Monohydrochloride as a Modulator on Apparent Viscosity of Sucrose-Based Multicomponent Gels

“…1 It is traditionally used as a gelling and thickening agent in the food and pharmaceutical industries. Apart from its known health benefits, 2 novel applications such as preparation of food matrixes containing added nutrients, 3 prebiotics, 4 modification of viscosity of other carbohydrates, 5 food preservation and packaging, 6 emulsion stabilizer, 7 biosorption, 8 targeted drug delivery, 9 controlled drug release, 10 preparation of drugencapsulated emulsion gels 11 and nanoparticles, 12 wound healing, 13 tissue engineering, 14 bioprinting, 15 energy storage, 16 etc. have been developed using pectin-based composites.…”