Introduction: A cross sectional study was carry out on the necessity of creating a poison control center in Cameroon, by evaluating the knowledge and perception of health care professionals in the Laquintinie hospital and the Bonassama District hospital in Douala, based on a 2 years (2014 to 2015) record files reviewed of poison victims.
Enterobacteriaceae are the most common causes of community-acquired and nosocomial infections. They are usually treated with beta-lactam antibiotics, i.e., penicillins, broad-spectrum cephalosporins, and carbapenems (Imipenem, meropenem, ertapenem). In order to evaluate the resistance profile of Escherichia coli (E. coli) to beta-lactam antibiotics, a 3-year retrospective study was carried out in the medical biology laboratory of the Laquintinie Hospital in Douala. The aim was to compare the behavior of Escherichia coli bacteria to penicillins, carbapenems, and cephalosporins. In order to achieve this objective, we used the results obtained from E. coli strains isolated from urine, cervico-vaginal fluid, puncture fluid, pleural fluid and pus samples. Identification was confirmed using the API 20 E Enterobacteriaceae system of biochemical testing and the VITEK 2 system. The VITEK 2 system was used to perform the antibiogram. Statistical analyses obtained from GraphPad Prim V 5.0 software allowed us to perform tests such as ANOVA, Kruskal-Wallis test, and Spearman correlations. Preliminary results show that E. coli bacteria are highly resistant to penicillins and significantly susceptible to cephalosporins of all generations if they are not ESBL (extended-spectrum beta-lactamases). Data on carbapenem behavior show less resistance and moderate susceptibility.
The main aim of this study was to determine the quality of vegetable oils consumed by the population in Yaoundé.
The study was carried out on 14 vegetable oils sampled following a survey. The antioxidant potentials of these oils were analyzed using the 2,2-diphenyl-1-picrylhydrazyl, FRAP total antioxidant capacity. The concentrations of some heavy metals (Pb, Cu, and Fe) were determined using flame atomic absorption spectrometry after acid digestion. The acid and peroxide values were assessed using methods described by the Cameroonian standard on vegetable oils.
The sample included 8 brands of which, 5 of refined palm oil, 4 of soybean oil, 1 brand of cottonseed oil, sunflower oil, and cold extracted olive oil, red palm oil bleached palm oil each. The antioxidant activity showed a good correlation, with red palm oil having the greatest antioxidant potential and bleached palm oil having the least antioxidant potential. The Pb, Cu, and Fe contents had the following conformities: 71.4%, 100% respectively. For the acid and peroxide values, we found 50% and 21.4%, respectively. Six of the fourteen (42.9%) analyzed oils contained less than 33 IU/g vitamin A.
These results highlight the poor quality of the oils consumed in Yaoundé.
Blood transfusion is a substitute therapy which consists to administered blood or one of its components to one or more sick person. hematological components retain their stability if the cold chain is maintained between 2 and 6°C. essential for good transfusion performance. The aim of this work was to evaluate the stability of haemogram parameters under the influence of cold chain on whole blood bags seronegative from infections transmissible by blood transfusion (ITT).
Methodology: A cohort of 200 blood collected into the citrate phosphate dextrose adenine (CPDA) blood bag collected consecutively at the blood transfusion center of the Douala General Hospital constituted the population of the study conducted from March 1st to September 30th, 2018. 5ml of this blood from the donor’s blood bag was collected into a dry tube (for the blood count using URIT 3000 Plus machine) and the rest was stored in a BIOBASE brand refrigerator at a temperature between 2 and 6. On the day of delivery, a second sample was taken by section of the tubing of the bag for the analysis of a second haemogram of the same blood bags. Temperature, refrigerator opening frequency and blood were collected every day. Data analysis was done using by SPSS 20.0 for Windows software. The results were considered significant at p˂0.05.
Results: The storage temperature significantly (p˂0.05) decrease the rate of leukocytes, erythrocytes and hemoglobin. When the shelf life and the frequency of opening the refrigerator increased, hemoglobin, hematocrit, MCV and lymphocyte decreased significantly while leukocyte, MCHC, thrombocytes and granulocytes increase significantly.
Conclusion: This study showed that, the decrease in leukocytes, red blood cells and hemoglobin levels was significantly related to shelf life and frequency of opening the refrigerator. Knowledge on this variation could be very useful in the selection of refrigerated blood or pint and the efficiency of transfusion.
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