Nanomaterials have been used for diagnosis and therapy in the human medical field, while their application in veterinary medicine and animal production is still relatively new. Nanotechnology, however, is a rapidly growing field, offering the possibility of manufacturing new materials at the nanoscale level, with the formidable potential to revolutionize the agri-food sector by offering novel treatment options for prevalent and expensive illnesses such as bovine mastitis. Since current treatments are becoming progressively more ineffective in resistant bacteria, the development of innovative products based on both nanotechnology and phytotherapy may directly address a major global problem, antimicrobial resistance, while providing a sustainable animal health solution that supports the production of safe and high-quality food products. This review summarizes the challenges encountered presently in the treatment of bovine mastitis, emphasizing the possibility of using new-generation nanomaterials (e.g., biological synthesized nanoparticles and graphene) and essential oils, as candidates for developing novel treatment options for bovine mastitis.

The utilization of sex-sorted sperm for artificial insemination and in-vitro fertilization is considered a valuable tool for improving production efficiency and optimizing reproductive management in farm animals, subsequently ensuring sufficient food resource for the growing human population. Despite the fact that sperm sex-sorting is one of the most intense studied technologies and notable progress have been made in the past three decades to optimize it, the conception rates when using sex-sorted semen are still under expectations. Assisted reproduction programs may benefit from the use of emergent nano and microfluidic-based technologies. This article addresses the currently used methods for sperm sex-sorting, as well as the emerging ones, based on nanotechnology and microfluidics emphasizing on their practical and economic applicability.

Measurement of antioxidant capacity represents an analytical major challenge in terms of accuracy, efficiency, rapid response, or low cost of detection methods. Quantification of antioxidant capacity of food samples using disposable screen‐printed microelectrodes (SPMEs) was based on cyclic voltammetry versus open-circuit potential (CV vs OCP) and differential pulse voltammetry (DPV) as compared with spectrophotometric measurement of the CUPRAC reaction with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox). The SPMEs are organic‐resistant electrodes and thus compatible with food samples and organic solvents used to dissolve trolox. A micropipette was used to release a drop of 50 μL sample on the spotted surface of the SPME sensor/working electrode that was time programmed to function according to the working protocol. The SPME response was linearly correlated with trolox content. This preliminary demonstration was focused on the analysis of tea infusions, due to the simplicity and reproducibility of the samples’ preparations involved. Analytical results of the antioxidant capacity (expressed as mol·L−1 trolox equivalents) of the tea samples showed a good agreement in the case of spectrophotometry and differential pulse voltammetry (R2 > 0.998). DPV with SPME based on CUPRAC reactions was proven to be a promising approach for the characterization of antioxidant capacity of tea samples with rapid response, cost-effectiveness, and simplicity of operation.

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ABSTRACT. Three groups of HolsteinFriesian cows were used to test the effect of two intravenous solutions on metritis, mastitis prevalence and reproductive performance. The first solution containing 3000 mg Butaphosphan (100 mg/ml) and 1.5 mg B12 Vitamin (0.05 mg/ml) was administered to BB12 group in the first 3 days after parturition and the second solution containing 3000 mg Butaphosphan (100 mg/ml), 1.5 mg B12 Vitamin (0.05 mg/ml) and 7 g of C vitamin (0.1 g/ml) was administered to BB12C group in the same period. The control group (C) didn't receive any intravenous solution. The TukeyKrammer multiple comparison tests were used to compare the results. The prevalence of clinical metritis, puerperal metritis and clinical mastitis was lower in the BB12C group (p< 0.05), compared to BB12 and C groups. Regarding the prevalence of metritis and mastitis no differences was observed in the BB12 group, compared with the C group (p>0.05). However, no difference was observed in the prevalence of the clinical endometritis (p>0.05) for the all three groups of cows. Also, the BB12C group registered the best calving to first insemination interval and calving to conception interval (p< 0.05). In conclusion, the intravenous cocktail containing Butaphosphan, B12 and C Vitamin can reduce the prevalence of some uterus and udder infection in the first 7 days after parturition in dairy cows.