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The research was conducted from August until November 2012 in the districts of Moldavia (Botoşani, Suceava, Iaşi, Vaslui, Bacău, Neamţ, Galaţi and Vrancea), in the West and the Central part of the country (Cluj and Covasna districts) and in the South-East of the country (Tulcea and Brăila districts). The choice of the districts was established based on the informations received from Animal Improvement and Breeding Offices in Romania. The purpose of this research was to identify the effective of Sura de stepă cattle breed in Romania and the evaluation of the phenotypic characters in steppe animals, which belong to the variety of Moldavian breed. The results showed that the Sura de stepă breed consists of a minor part, being raised in just two districts of Moldavia, Iaşi and Neamţ, respectively, as a pure breed with an average of 0,03 % (83 cows), at the Research and Development Station for Cattle Breeding (R.D.S.C.B.) Dancu-Iaşi, with a core of preservation of 59 cows (0,19 %) and at the Holding TCE 3 Brazi Society, Neamţ district, with a core of 24 cows (0,06 %), and under half-breed form with an average of 0,33 % (592 cows). In Harghita, Covasna and Cluj districts, 295 cows from the Sura de stepă breed, Hungarian variety, were identified, excepting being the animals belonging to University of Agricultural Sciences and Veterinary Medicine Cluj, which come from R.D.S.C.B. Dancu-Iaşi, which are Romanian Sura de stepă, Moldavian variety. In Tulcea district, 20 cows of half-breed Sura de stepă and 50 cows of pure breed cattle, Moldavian variety, were identified, from which 24 cows at a private landlord in Pardina locality, Tulcea district, and 26 cows belonging to nine owners from different localities (C.A. Rosetti, Pardina, Chilia Veche, Sfântu Gheorghe, Crişan), each owning 2-3 cows. The analysis of the main body indices showed that the Sura de stepă cows from Pardina, Tulcea county, are of small size and weight, having smaller values in all analysed parameters, compared to the Sura de stepă cows from R.D.S.C.B. Dancu-Iaşi from the North-East part of the country, which however fitted the specific limits of the breed, Moldavian variety.

The early resumption of estrous cycles following calving is important for high reproductive efficiency, but also for the cows which haven't been observed in estrus during the first 60 days after calving, the last ones having a significantly higher risk of being culled than cows that have displayed estrus .The aim of this study was to determine the effect of hormonal treatment on reproduction period in cows, and also to establish if there is any significant difference in some reproductive parameters between cows treated with hormonal medication and cows which resumed ovarian cyclicity before 60 days postpartum.This study was performed in a dairy cow's farm in North-Eastern Moldavia, over a period of 2 consecutive years, on Romanian Black Spotted cows, in order to determine hormonal treatments influence over reproduction period. Hormonal treatments used for the above mentioned diseases were represented by luteolytic agent prostaglandin F2α, one of its potent analogues (Proliz, Biotur, Romania) and by administration of exogenous gonadotrophin releasing hormone (Receptal, Intervet, Romania).We noticed that average reproduction period (52.1 days) and the other breeding intervals that were taken under study (over a two years period), presented different values for cows that have been under hormonal medication treatment, compared to those which resumed ovarian cyclicity before 60 days postpartum (52.7 days).Thus, this preliminary study supports the development of a more detailed study on several dairy cows' farms in order to investigate the answer of different types of hormonal treatments against persistent corpus luteum and cystic ovarian disease.

The study was performed inside a dairy cows farm in NorthEastern Moldavia, over a period of 4 consecutive years (2010-2013), on a number of 1153 Romanian Black Spotted cows in order to determine the prevalence of different ovarian diseases by year, season and lactation number. Evaluation of ovarian diseases prevalence was based on data gathered from gynaecological cows records which were took as reference. The results showed different pathological entity variation. Thus, 27.84% of the animals taken into study presented ovarian diseases, of which 13.53% are represented by persistent corpus luteum, followed by ovarian hypofunction (7.98%) and a value of 6.33% which was recorded for cystic ovarian disease. From the recorded data analysis, it has been concluded that from the total number of ovarian diseases taken into study, the highest prevalence was registered for persistent corpus luteum (48.6%), followed by ovarian hypofunction (28.66%) and cystic ovarian disease (22.74%). In relation to the different influence factors, ovarian diseases prevalence in dairy cows showed variations depending on the year of study, with mean values ranging from 20.56% (2012) to 30.22% (2010). In conclusion it is considered that the prevalence of ovarian diseases in dairy cows is recording some peak values during the cold season because of the winter feeding (high proportion of silage), lack of physical exercise and protein-rich diets for increasing the milk yield. Also, prolonged period of uterine involution (uterine subinvolution) makes the secretion of luteolytic hormone (PGF 2α) to be lowered in detriment of PGE2.

ABSTRACT. Three groups of HolsteinFriesian cows were used to test the effect of two intravenous solutions on metritis, mastitis prevalence and reproductive performance. The first solution containing 3000 mg Butaphosphan (100 mg/ml) and 1.5 mg B12 Vitamin (0.05 mg/ml) was administered to BB12 group in the first 3 days after parturition and the second solution containing 3000 mg Butaphosphan (100 mg/ml), 1.5 mg B12 Vitamin (0.05 mg/ml) and 7 g of C vitamin (0.1 g/ml) was administered to BB12C group in the same period. The control group (C) didn't receive any intravenous solution. The TukeyKrammer multiple comparison tests were used to compare the results. The prevalence of clinical metritis, puerperal metritis and clinical mastitis was lower in the BB12C group (p< 0.05), compared to BB12 and C groups. Regarding the prevalence of metritis and mastitis no differences was observed in the BB12 group, compared with the C group (p>0.05). However, no difference was observed in the prevalence of the clinical endometritis (p>0.05) for the all three groups of cows. Also, the BB12C group registered the best calving to first insemination interval and calving to conception interval (p< 0.05). In conclusion, the intravenous cocktail containing Butaphosphan, B12 and C Vitamin can reduce the prevalence of some uterus and udder infection in the first 7 days after parturition in dairy cows.

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