Pilot-scale Bacillus thuringiensis based biopesticide production (2000 L bioreactor) was conducted using starch industry wastewater (SIW) as a raw material using optimized operational parameters obtained in 15 L and 150 L fermenters. In pilot scale fermentation process the oxygen transfer rate is a major limiting factor for high product yield. Thus, the volumetric mass transfer coefficient (Ka) remains a tool to determine the oxygen transfer capacity [oxygen utilization rate (OUR) and oxygen transfer rate (OTR)] to obtain better bacterial growth rate and entomotoxicity in new bioreactor process optimization and scale-up. This study results demonstrated that the oxygen transfer rate in 2000 L bioreactor was better than 15 L and 150 L fermenters. The better oxygen transfer in 2000 L bioreactor augmented the bacterial growth [total cell (TC) and viable spore count (SC)] and delta-endotoxin yield. Prepared a stable biopesticide formulation for field use and its entomotoxicity was also evaluated. This study result corroborates the feasibility of industrial scale operation of biopesticide production using starch industry wastewater as raw material.
The aim of this study was to produce Bacillus thuringiensis based biopesticide using starch producing industry wastewater (SIW) fortified with soybean medium and optimize the formulated product using different adjuvants. This study was necessary as low endotoxin concentration is obtained in formulated biopesticide when SIW alone is used as fermentation medium. The fermentation runs were conducted using SIW alone and SIW fortified with 25% soybean (w/v) medium in 2000 L and 150 L bioreactor respectively. SIW supplemented with soybean medium showed an increase in cell count (from 1.95 x 10 8 CFU/mL to 1.65 x 10 9 CFU/mL), spore synthesis (from 1.5 x 10 8 CFU/mL to 1.35 x 10 9 CFU/mL) and endotoxin concentration (from 436 µg/mL to 1170 µg/mL) when compared to SIW medium alone. The fermented broth was concentrated using continuous centrifugation and adjuvants were added for biopesticide formulation in order to enhance its resistance against UV rays and rainfastness. Entomotoxicity of the formulation produced using fermented broth of SIW fortified with soybean (38000 IU/ µL) was higher than that obtained by SIW medium alone (21000 IU/ µL), commercial biopesticide Foray 76B (20000 IU/ µL) and Btk sander's (12500 IU/ µL).
Lignocellulosic biomass is both low cost and abundant, and unlike energy crops, can escape associated ethical dilemmas such as arable land use and food security issues. However, their usage as raw material in a biorefinery implies an inherent upstream pretreatment step to access compounds of interest derived from lignocellulosic biomass. Importantly, the efficiency of this step is determinant for the downstream processes, and while many pretreatment methods have been explored, extrusion is both a very flexible and promising technology. Extrusion is well-known in both the polymer and pharmaceutical industries and has been used since the 18th century. However, as a pretreatment method for lignocellulosic biomass, extrusion is relatively new. The first use for this purpose dates back to the 1990s. Extrusion enjoys a high degree of flexibility due to the many available parameters, but an understanding of extrusion requires a knowledge of these parameters and the different relationships between them. In this paper, we present a concise overview of lignocellulosic biomass extrusion by reviewing key extrusion parameters and their associated extruder design components and operating conditions.
In this study, cost simulation was made to produce Bacillus thuriengiensis based biopesticide formulation using starch industry wastewater (SIW) as substrate. The results obtained at pilot plant (2000L capacity fermenter) were used for cost simulation of the process. The unit production cost for annual production of 5 million L of formulated biopesticide (20.2 Billion International Units (BIU)/L) was estimated to be $ 2.54/L, which is competitive to chemical pesticides. The techno-economic evaluation revealed that the profitability of the biopesticide manufacturing process was sensitive to the plant capacity and selling price of the biopesticide. The manufacturer should target 5 million L annual plant capacity and selling price of $ 15/L for payback period to be less than 5 years. The process serves many advantages ( 1) alternate disposal or bio-valorisation of industry wastewater and (2) use of industry wastewater as inexpensive carbon source reducing cost of raw materials for fermentation.
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