A molecularly imprinted polymer (MIP) with dual dopamine/serotonin-like binding sites (DS-MIP) was synthesized for use as a receptor model of study the druginteraction of biological mixed receptors at a molecular level. The polymer material was produced using methacrylic acid (MAA) and acrylamide (ACM) as functional monomers, N,N′-methylene bisacrylamide (MBAA) as cross-linker, methanol/water mixture (4:1, v/v) as porogen and a mixture of dopamine (D) and serotonin (S) as templates. The prepared DS-MIP exhibited the greatest rebinding of the template(s) in aqueous methanol solution with decreased recognition in acetonitrile, water and methanol solvent. The binding affinity and binding capacity of DS-MIP with S were found to be higher than those of DS-MIP with D. The selectivity profiles of DS-MIP suggest that the D binding site of DS-MIP has sufficient integrity to discriminate between species of non-optimal functional group orientation, whilst the S binding site of DS-MIP is less selective toward species having structural features and functional group orientations different from S. The ligand binding activities of a series of ergot derivatives (ergocryptine, ergocornine, ergocristine, ergonovine, agroclavine, pergolide and terguride) have been studied with the DS-MIP using a competitive ligand binding assay protocol. The binding affinities of DS-MIP were demonstrated in the micro-or submicro-molar range for a series of ergot derivatives, whereas the binding affinities were considerably greater to natural receptors derived from the rat hypothalamus. The DS-MIP afforded the same pattern of differentiation as the natural receptors, i.e. affinity for the clavines > lysergic acid derivatives > ergopeptines. The results suggest that the discrimination for the ergot derivatives by the dopamine and serotonin sites of DS-MIP is due to the structural OPEN ACCESSInt. J. Mol. Sci. 2008, 9 2334 features and functional orientation of the phenylethylamine and indolylethylamine entities at the binding sites, and the fidelity of the dopamine and serotonin imprinted cavities.
A molecularly imprinted polymer (MIP) enantioselective receptor for the (R)-thalidomide enantiomer was synthesized and evaluated for its ability to deliver the drug to cancer cells. Polymer networks with precisely engineered binding sites were built into the assembled nanoparticles by a self-organizing template in the prepolymerized mixture using methacrylic acid, a fluorescently active 2,6-bis(acrylamido)pyridine and N,N 0 methylene-bis-acrylamide, via both a covalent approach and a physical approach. The fine-tuning of particle diameters was carried out by changes to the polymerizing synthesis method, the type of solvent and the amount of the poloxamer that led to an optimal formulation of the nanoparticles with sizes as small as 100 nm. Data from the 1 Hnuclear magnetic resonance spectroscopy revealed the important structural motifs of an (R)-thalidomide-selective cavity for two different polymerization processes. We have investigated their ability for enantiomer recognition and the potential ability to protect the chiral MIP with a self-assembled poloxamer structure. Moreover, the effect of the smaller molecular size can not only enable favorable imaging properties but also facilitate enhanced green fluorescence intensity for the deposited MIP and the (R)-thalidomide in the poloxamer nanoparticles in a cell-line in which the grafted MIP being higher than the deposited one. It was also demonstrated that the deposited MIP nanoparticles had the potential to make the drug effective for attacking multidrug-resistant cells. Thus, the poloxamer nanoparticles containing a thermoresponsive MIP could maximize the release of the nontoxic (R)-thalidomide at the tumor tissue, with the help of a proper temperature shift at the site.
We present details of the chemical surface properties of the molecularly imprinted polymer (MIP) on quartz crystal microbalance (QCM) for the tracking of the chiral recognition of racemic thalidomide and its (R)-enantiomer. We investigate the assembly and specific patterns of enantiomer and racemate of thalidomide on the poly(urethane) coating consisted of the syntheticestrogen bisphenol A (BPA) on a QCM electrode by infrared spectroscopy and atomic force microscopy (AFM), which confirmed the surface properties of these materials. The BPA present on the surface of the coating layer revealed a positive frequency response for the racemic thalidomide that eventually appeared. This involved a negative shift of 80 Hz for a 200 mg mL 21 racemic thalidomide, and in all cases, a negative shift of 200 Hz for a 100 mg mL 21 (R)-thalidomide. The affinity constants (K a ) for the racemate adsorbed onto the polymer layer imprinted with (R)-thalidomide were lower than those for the (R)-thalidomide. Also, the binding energy involved a different binding process of the chiral forms and indicated that the two enantiomers had a twofold difference in their binding energies. Thus, the advantage of the use of BPA is proven that will function as hydrogen-bond donors in the enantioselective recognition site of the MIP. The data of functional analysis demonstrated that the biomimetic detection using molecular imprinting turn out to a study of the pharmaceutical effects of a pharmaceutically chiral compound on natural receptor functions. This approach is highly useful that highlight an enhanced understanding of the mechanism of stereochemistry required for biological controls.
The aim of this research is to study the synergistic effect on the nitric oxide scavenging activity of mangosteen pericarp and the stingless bee (Tetragonula laviceps) propolis extracts and their phytochemical constituents. The propolis and mangosteen pericarp were extracted by reflux method with ethanol. TPC and TFC of propolis extract were 123.73±2.80 mg GAE/g extract and 70.65±11.21 mg QE/g extract, respectively, and mangosteen pericarp extract was 387.93±15.10 mg GAE/g extract and 87.00±5.06 mg QE/g extract, respectively. The ESI-LC-MS data displayed that both extracts have a variety of phytochemical constituents, such as xanthones, flavonoids, and miscellaneous. The synergistic effect of Nitric oxide scavenging activities of propolis and mangosteen pericarp extracts showed higher activity than individual extracts with various concentrations. Thus, the synergistic effect of propolis and mangosteen pericarp extracts may be an alternative source of inflammatory drug development in the future.
Bananas are one of the most popular fruits in the world, yet only around 12% of them are consumed, posing an environmental problem. The goal of this research is to extract Hom Thong banana cellulose, which is the major component of banana peels. Fat analysis was used to extract and bleach Hom Thong banana cellulose, followed by soaking in 15% hydrogen peroxide for 3 h. The Hom Thong banana peel cellulose was washed and dried at 60 °C for 10 h. The obtained Hom Thong banana cellulose was characterized in terms of fatty acid profile, intermolecular interactions, and thermal analysis by using gas chromatography, FT-IR, and DSC techniques, respectively. The results showed that the content of palmitic acid (C16:0) in the post-evaporated ethanolic extract is larger than in pre-evaporated ethanolic extract, with a ratio of 44.91% and 38.62%, respectively. At a ratio of 26.19% and 31.56%, the post-evaporation of the ethanolic extract contained less linoleic acid (18:2cis) than the pre-evaporation of ethanolic extract. Intra-molecular interactions between OH groups of cellulose were shown by FT-IR spectra. DSC thermograms revealed that the extracted cellulose had good thermal characteristics and was appropriate for the food and cosmetic industries.
Meat adulteration has become a serious problem in global which directly affects to food consumers and producers. Therefore, it requires a tool to authenticate meat species to ensure safety of food products. Next generation sequencing (NGS) coupled with ribosomal RNA mitochondrial DNA gene can be used to analyze mixture of meat species in multiple meat samples. Therefore, this study aims to utilize NGS coupled with rRNA gene to identify 4 meat species (cattle, chicken, fish, and pig). Three primer sets (12S-Ki, 16S-KH, and 16S-Ki) were used to amplify DNA from the four meat species. All primer sets could be successfully amplified DNA fragments which corresponded to their size expectation. 16S-KH showed better detection effect in all species comparing with others. While the 12S-Ki and 16S-Ki could not be used to amplify in fish and chicken species. This may occur due to mismatches between sequences of primers and annealed regions of these species. Library construction of all PCR amplicons were prepared and sequenced by NGS. Amplicons amplified by 12S-Ki (fish) and 16SKi (chicken and fish) could not be mapped to the database because no PCR amplicons could not be amplified. NGS coupled with 16S-KH was then evaluated for precision test. The experimental precision was directly investigated comparing the results obtained from libraries that derives from DNA of four meat species which separately amplified for 3 different runs. As expected, the number and proportion of mapped reads between three different runs were also concordant. The percentage of mapped reads ranged from 14.05% to 31.04%, 15.14% to 31.98%, and 14.21% to 33.05% (1st, 2nd, and 3rd run, respectively). This demonstrated that NGS coupled with rRNA mtDNA gene could be reliably implemented as a routine testing. This developed technique can be applied to control and monitor meat adulterations in halal meat production and industry.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.