No abstract

Clove (Syzygium aromaticum (L.) Merr & Perry) is one of the plants used as medicine, society used leaves as anti-inflammatory drugs at the time of toothache, which at the leaf contained flavonoids. The aim of this study is to determine the content of total flavonoid of ethanol extract clove leaf (EECL). Clove leaf was extracted using maceration method with ethanol as a solvent during at 3 x 24 hours. Analysis qualitative of flavonoids compound using thin layer chromatography method. Determination of total flavonoids content of clove leaves using spectrophotometri UV-Vis method λ 418 nm. The result of the research with rendamen yield of EECL were 16,1134%, and total flavonoids content is 73,08 mgRE/g extract with persentation 7,308%.

Cordia myxa L .leaf is a plant from Boraginaceae family which is traditionally used as medicine of several skin diseases . The purpose of thid research is to know the chemical compound antioxidant activity of ethanolic extract of Cordia myxa L. leaf. The used extraction method was maceration method using 70% as solvent. Identification of coumpound group used color reaction and precipitation method respectively. While antioxidant activity test used free radical scavenging method of DPPH (1,1-dyphenyl-2 pycril Hydrazil). From the extraction result, that was found that %redamen of ethanolic extract of Cordia myxa L .leaf 2.76%. While the determination of chemical compound showed that ethanolic extcract of Cordia myxa L .leaf containing steroid, flavonoid, saponin, and phenol. The result antioxidant activity assay showed that ethanolic extract of Cordia myxa L .leaf has intermediate free antiradical activity with IC50 value 54.92 µg/mL. But it is potensial is lower than Quarcetin which has IC50 value 0.52 µg/mL.

Teh hijau memiliki nama spesies Camellia sinensis (L.) Kuntze, family Theaceae dan Jati belanda (Guazuma ulmifolia Lam.) termasuk kedalam family sterculiaceae Tujuan Penelitian ini adalah melakukan standarisasi ekstrak air daun jati belanda dan ekstrak air the hijau. Ekstrak distandardisasi dengan beberapa dua parameter yaitu parameter spesifik dan parameter non spesifik. Kadar sari larut air pada jati belanda 12,88 % dan teh hijau 40,88, sedangkan kadar sari larut etanol pada jati belanda 4, 23 % dan pada teh hijau 4,23 %. Hasil pengujian kandungan kimia menunjukkan pada ekstrak jati belanda mengandung saponin dan flavonoid sedangkan pada teh hijau mengandung tannin dan flavonoid. Kadar air ekstrak daun jati belanda 0,95 % dan teh hijau 2,79%. Hasil kadar abu total jati belanda sebesar 37,61% dan teh hijau 36,84 %. Kadar abu tidak larut asam yaitu pada jati belanda sebesar 3,54% dan teh hijau 3,77%. Hasil dari penetapan susut pengeringan pada ekstrak jati belanda yaitu 0,46 % dan teh hijau 0,46 %. Ekstrak jati belanda maupun teh hijau berdasarkan pengujian standarisasi meliputi parameter spesifik dan no-spesifik memenuhi standarisasi mutu bahan baku.

Indonesia people have been used of Bay Leaves (S. polyanthum [Wight.] Walp.) to treat of antidiarrhea, cholesterol, hypertension, gastritis, and diabetes mellitus. Extrac of Bay Leaves can be inhibit growth of Escherichia coli, Vibrio Cholera, and Salmonella sp. It's the bacterium that produce an entherotoxin so as to cause diarrhoea. Bioassay of antidiarrheal activity by using transit intestinal method and mice (Mus musculus) as an animal model induced by castor oil. The mice were grouped into five groups. Group I is received Na-CMC as control, group II, III, and IV are received of Ethanolic Extract of Bay Leaves (EEBL) with concentration 10%, 20%, and 30%, respectively and group V as positive control by received Loperamide-HCl. The charcoal used to marker as indicators. The result of the test indicate that EEBL have antidiarrheal activity to animal model. Refers to analysis of variant p=0.05, showing that concentration 30% has an activity non significant with loperamide-HCl as positive control.

Phytochemical Screening and Determination of Total Flavonoids Content of Methanolic Extracts of Boroco Herbs (Celocia argentea L).Boroco plant is often used as a traditional medicine for anti inflammatory, diuretic, hypertension, dysentery. Chemical constituents in boroco herbs (Celocia argentea L.) are alkaloids and flavanoids, glycosides, tannin and saponin (2003) . Each sample extraction using 96 % methanol of maceration method. Then performed to determine the class of phytochemical screening of active compounds are contained in the sample. Determination of total flavanoids done by chang et al method ( 2002) of Boroco herbs. Result of the study showed the total flavanoids content of methanolic extract of boroco herbs calculated as a rutin for 2.57%.

ABSTRAKTeh hijau memiliki nama spesies Camellia sinensis (L.) Kuntze, family Theaceae dan Jati belanda (Guazuma ulmifolia Lam.) termasuk kedalam family sterculiaceae. Ekstrakse dengan metode maserasi termodifikasi microwave dengan pelarut air. Purifikasi ekstrak dengan menggunakan pelarut dengan tingkat kepolaran yang berbeda-beda dimulai dengan petroleum eter, kloroform, etil asetat dan metanol. Pengujian aktivitas antioksidan dengan metode peredaman radikal DPPH. Hasil penelitian dipeoleh rendamen ekstrak terpurifikasi ekstrak teh hijau 13,33 % dan jati belanda 46,66 %.Pengujian antioksidan menunjukkan nilai IC50 jati belanda yaitu 10,13 ppm, lebih besar dibandingkan teh hijau yaitu 31,13 %.

The objective of this study was to evaluate on the estrus initial, duration of estrus and pregnancy rate following of prostaglandin F 2 alpha injection in heifers and cows. A total of 60 Bali cows were divided into two group, first group was contain 30 heifers and second group comprise 30 cows. The weight of cows were 300±6.87 and 365±8.14kg, aged approximately 2 years and 3-6 years for heifers and cows, respectively. All the heifers and cows were normal cycling with showing regular cyclic activities and having active of corpus luteum. The parameters in this study was determine including the time required for onset of estrus,duration of estrus and pregnancy rate. The result showed that percentage estrus response, duration of estrus and pregnancy rate were non significant difference (P>0.05) between heifers and bali cows after estrus synchronization with single dose of prostaglandin F 2 α. It was concluded that injection of PGF2α on heifers and bali cows was similar result on the time required for onset of estrus, duration of estrus and pregnancy rate.