Antidiabetic xanthones with α-glucosidase inhibitory activities from an endophytic Penicillium canescens

Malik, Abd.; Ardalani, Hamidreza; Anam, Syariful; McNair, Laura F.; Olsen, Kresten Jon Kromphardt; Frandsen, Rasmus John Normand; Franzyk, Henrik; Kongstad, Kenneth T.

doi:10.1016/j.fitote.2020.104522

Cited by 40 publications

(24 citation statements)

References 39 publications

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“…H. perforatum hairy roots are unique model for production of xanthones and previous HPLC analyses clearly demonstrated that γ-mangostin, mangiferin, and other prenylated and oxygenated xanthones in H. perforatum HR cultures are common defense compounds in response to Agrobacterium transformation (17). Several studies of plant extracts rich with γ-mangostin and prenylated and oxygenated xanthones report strong hypoglycemic effects in STZ diabetic rats (9,10,11). One of the many pharmacological effects of mangiferin is the reduction of plasma glucose in different animal models with diabetes (7) via several unique mechanisms including inhibition of glucosidase (22), which could prevent overloaded carbohydrates from being converted to glucose and absorbed in the intestine.…”

Section: Discussionmentioning

confidence: 99%

“…The H. perforatum extracts contain naphthodianthrones, flavonol glycosides, biflavonoids, phloroglucinol derivatives, and xanthones (4) that are responsible for antidepressant, anti-inflammatory, antiviral, anticancer, antibacterial, and antidiabetic activities (6). Among these bioactive compounds, xanthones are known as the most potent antidiabetic agents (7,8,9,10,11).…”

Section: Introductionmentioning

confidence: 99%

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Hypericum Perforatum L. Hairy Root Extracts – Regulation of Glycemic, Metabolic, Serum Enzyme and Lipid Profile in STZ - Induced Diabetic Rats

Rafailovska

Tushevski

Simic

et al. 2022

Macedonian Veterinary Review

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Apart from currently available therapeutics for the treatment of diabetes mellitus, much attention has been paid to discover phytochemicals from natural resources, mainly due to their low side-effects. Hypericum perforatum hairy root (HR) transformed with Agrobacterium rhizogenes A4 represent prospective experimental system enriched in xanthones, known as potent antidiabetic agents. Thus, the aim of this study was to evaluate HR extracts for their potential antihyperglycemic activity in streptozotocin (STZ)-induced diabetic rats, also compared to the effects of wild-growing Hyperici herba (HH). We conducted an acute-toxicity study, multiple dose study, and 24h blood glucose measurements after a single dose administration of HH and HR (200 mg/kg) in diabetic rats. Furthermore, we examined the effects of 14-days administration of HH and HR extracts on blood glucose levels, metabolic parameters, enzyme, and lipid status in healthy and diabetic rats. Both extracts produced a fall of about 70% in blood glucose level after 24h of administration. Two-week treatment with HH and HR induced a significant decrease (70-72%) in blood glucose levels. Moreover, we found an improvement of the dysregulated metabolic parameters (body weight, food, and water consumption and urine output). Serum enzyme (AST, ALT, and γ-GT) and lipid profile parameters (CHOL, TAG, and HDL) were also improved by both extracts. These findings might provide a new insight for managing diabetic hyperglycemia and dysregulated serum enzyme and lipid profile, using extracts from transgenic roots cultures from H. perforatum.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hypericum Perforatum L. Hairy Root Extracts – Regulation of Glycemic, Metabolic, Serum Enzyme and Lipid Profile in STZ - Induced Diabetic Rats

Rafailovska

Tushevski

Simic

et al. 2022

Macedonian Veterinary Review

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“…Jamur endofit merupakan mikroorganisme yang terdapat dalam jaringan tumbuhan yang bersifat mutualisme terhadap tumbuhan inang dimana mikroorganisme ini tidak menyebabkan kerusakan pada tumbuhan inangnya (Jia et al, 2016;Malik et al, 2020). Jamur endofit telah diketahui berperan sebagai sumber metabolit yang memiliki beberapa aktivitas biologi seperti antimikroba, antikanker, antivirus, antijamur, dan antioksidan (Jalgaonwala et al, 2017).…”

Section: Pendahuluanunclassified

“…Prosedur isolasi jamur endofit menggunakan metode tanam langsung mengikuti (Hasiani, 2015;Malik et al, 2020) dengan sedikit modifikasi yaitu sampel berupa daun, batang dan akar dicuci dengan air suling steril, direndam etanol 70% selama 2 menit, larutan natrium hipoklorit 1% selama 2 menit dan terakhir dibilas dengan air suling steril sebanyak tiga kali.…”

Section: Isolasi Jamur Endofitunclassified

Isolation of endophytic fungi from benalu batu (Begonia Medicinalis) and their toxicity on Artemia Salina

Anam

Syamsidi

Musyahidah

et al. 2022

JIF

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Background: Endophytic fungi are microorganisms found in plant tissues that are mutually beneficial to the host plant. They are known as sources of bioactive metabolites which possess various biological activities such as antimicrobial, anticancer, antiviral, antifungal, and antioxidant. Benalu batu (Begonia medicinalis) has been used empirically as a medicinal plant and has shown anticancer activity in breast cancer cells (T47D cells) and cervical cancer cells (HeLa cells). Objective: This study aimed to isolate the endophytic fungi from benalu batu and determine the toxicity activity and Lethal Concentration 50% (LC50) value of isolated endophytic fungi using shrimp larvae (Artemia salina). Method: Endophytic fungi were isolated from fresh parts; leaves, stems, roots of Benalu Batu. The selected parts were sterilized with ethanol 70% and sodium hypochlorite 1% subsequently placed into Potato Dextrose Agar Chloramphenicol (PDAC) then incubated at 28°C for 7 days. Isolated endophytic fungi were extracted by sonication method using ethyl acetate. The toxicity activity of isolated endophytic fungi was determined using the Brine Shrimp Lethality Test (BSLT). Results: Isolation of the endophytic fungi from benalu batu obtained 10 isolates, which the leaves part obtained 6 isolates, the stem obtained 3 isolates and the root obtained 1 isolate. The results of screening for toxicity test of 10 isolates at concentrations of 500 µg/mL shown that isolate D3, D5, and D6 gave 100% mortality. The LC50 values of isolate D3, D5, and D6 were obtained respectively in 448.03, 8.784, and 9.13 µg/mL. Conclusion: These results indicated that isolate D5 exhibits the greatest toxicity and needs to be investigated the activity for testing on cancer cells. Keywords: endophytic fungi, Begonia medicinalis, toxicity, Artemia salina

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“…MGP11 [16]. Three xanthones as α-glucosidase inhibitors were isolated from an endophytic Penicillium canescens [17].…”

Section: Introductionmentioning

confidence: 99%

Novel Antioxidants and α-Glycosidase and Protein Tyrosine Phosphatase 1B Inhibitors from an Endophytic Fungus Penicillium brefeldianum F4a

Bai

Zhang

et al. 2021

JoF

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Oxidative stress plays a very important role in the progression of diabetes and its complications. A therapeutic agent that is both antidiabetic and antioxidant would be the preferred choice for the treatment of diabetes. The crude extract of the endophytic fungus Penicillium brefeldianum F4a has significant antioxidant and α-glycosidase and protein tyrosine phosphatase 1B (PTP1B) inhibition activities. Chemical investigation of P. brefeldianum F4a using an activity-guided isolation led to the discovery of three new compounds called peniorcinols A–C (1–3) along with six known compounds: penialidins A (4), penialidin F (5), myxotrichin C (6), riboflavin (7), indole-3-acetic acid (8), and 2-(4-hydroxy-2-methoxy-6-methylphenyl) acetic acid (9). Their chemical structures were established by their NMR and HRESIMS. The absolute configurations of 1 and 3 were determined by experimental and calculated electronic circular dichroism (ECD). Their antioxidant activities were evaluated by DPPH• and ABTS•+ scavenging assays. Compounds 1–6 and 8–9 showed moderate to strong free radical scavenging activities. Significantly, 4–6 exhibited more potent ABTS•+ scavenging activity than that of the positive control. Their α-glycosidase and PTP1B inhibition activities were tested. Among them, compound 3 showed α-glucosidase inhibition activity, and compounds 7 and 8 showed PTP1B inhibitory activity for the first time. It is worth noting that 3 and 8 displayed both antioxidant and α-glycosidase or PTP1B inhibition activities. These finding suggest that compounds 3 and 8 could be used as lead compounds to generate new potent drugs for the treatment of oxidative stress-related diabetes.

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Antidiabetic xanthones with α-glucosidase inhibitory activities from an endophytic Penicillium canescens

Cited by 40 publications

References 39 publications

Hypericum Perforatum L. Hairy Root Extracts – Regulation of Glycemic, Metabolic, Serum Enzyme and Lipid Profile in STZ - Induced Diabetic Rats

Hypericum Perforatum L. Hairy Root Extracts – Regulation of Glycemic, Metabolic, Serum Enzyme and Lipid Profile in STZ - Induced Diabetic Rats

Isolation of endophytic fungi from benalu batu (Begonia Medicinalis) and their toxicity on Artemia Salina

Novel Antioxidants and α-Glycosidase and Protein Tyrosine Phosphatase 1B Inhibitors from an Endophytic Fungus Penicillium brefeldianum F4a

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