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Preparation of novel antibacterial and cytocompatible polyurethane membranes as occlusive dressing, which can provide moist and sterile environment over mild exudative wounds is considered in this work. In this regard, an epoxy-terminated polyurethane (EPU) prepolymer based on castor oil and glycidyltriethylammonium chloride (GTEAC) as a reactive bactericidal agent were synthesized. Polyurethane membranes were prepared through cocuring of EPU and different content of GTEAC with 1,4-butane diamine. The physical and mechanical properties, as well as cytocompatibility and antibacterial performance of prepared membranes were studied. Depending on their chemical formulations, the equilibrium water absorption and water vapor transmission rate values of the membranes were in ranges of 3-85% and 53-154g m(-2) day(-1), respectively. Therefore, these transparent membranes can maintain for a long period the moist environment over the wounds with low exudates. Detailed cytotoxicity analysis of samples against mouse L929 fibroblast and MCA-3D keratinocyte cells showed good level of cytocompatibility of membranes after purification via extraction of residual unreacted GTEAC moieties. The antibacterial activity of the membranes against Escherichia coli and Staphylococcus aureus bacteria was also studied. The membrane containing 50% GTEAC exhibited an effective antibacterial activity, while showed acceptable cytocompatibility and therefore, can be applied as an antibacterial occlusive wound dressing.

Preparation and evaluation of new polyurethane membranes for wound dressing application was considered in this work. The membranes were prepared through amine curing reaction of epoxy-terminated polyurethane prepolymers and an antibacterial epoxy-functional quaternary ammonium compound (glycidyltriehtylammonium chloride, GTEACl. To render the prepared membranes to be highly absorptive of wound exudates, poly (ethylene glycol) polyols were introduced into the polyurethane networks. Evaluation of biocompatibity via both MTT assay and direct contact with two different cell lines (fibroblast and epidermal keratinocytes) reveled that membranes with appropriate loading of GTEACl showed proper biocompatibility. Promising antibacterial activity of the prepared membranes against Staphylococcus aureus and Escherichia coli bacteria was confirmed by both agar diffusion and shaking flask methods. The membranes with balanced crosslink density and ionic groups' concentration possessed appropriate hydrophilicity and water vapor transmission rate; therefore, they could prevent the accumulation of exudates and decrease the surface inflammation in the wounded area.

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