Methods commonly used to determine the level of infestation of soil with zoosporangia of Synchytrium endobioticum are often laborious and call for complex equipment and costly and toxic chemicals. After comparative study of various existing methods, the authors propose a simple method based on centrifugation of soil residues in salt solutions of different density.
Characterization of amino acid content of grain of new wheat varieties and lines H. M. Hospodarenko, V. P. Karpenko, V. V. Liubych, V. V. Novikov Cultivation of potato leafroll virus (PLRV) in mammalian continuous cell lines I.V. Volkova, L.M. Reshotko, T.O. Bova, O.O. Dmytruk, S.V. Derevianko Formation of seed productivity and sowing qualities of red clover seed depending on the effect of lime, mineral and water-soluble fertilizers S. F. Antoniv, S. I. Kolisnyk, O. A. Zapruta Physical-chemical composition and technological properties of demineralized milk whey received by membrane methods I. O. Romanchuk, A. V. Minorova, N. L. Krushelnytska Impact of feeding male rats F 2 with different doses of germanium citrate on the content of microelements in the tissues and organs R. S. Fedoruk, U. I. Tesarivska, M. I. Khrabko, M. M. Tsap, H. H. Denys Formation of erosion resistance of gray forest soils in the conditions of Carpathian region O. Y. Kachmar, O. V. Vavrynovych, A. O. Dubytska, V. Ya. Ivaniuk Viability of sperm cells of boars at the addition of fi nely dispersive silica to cryopreservation and defrosting media O. V. Shcherbak, S. I. Kovtun Strategies of decreasing harmfulness of fusariosis agents in agrophytocenoses V. V. Schwartau, O. L. Zozulia, L. M. Mykhalska, O. Yu. Sanin 27 3 12 33 19 Contents 40 47 54 60 Державне видавництво «Аграрна наука» Національної академії аграрних наук України з 2018 року є членом Міжнародної асоціації видавців наукової літератури (PILA) та бере участь у проекті CrossRef на правах резидента.CrossRef є незалежною асоціацією, заснованою та керованою видавцями. Основним завданням CrossRef є організація доступу користувачів до первинних публікацій, що містять науковий контент, та сприяння колективній роботі видавців.Ідентифікатор цифрового об'єкта (DOI) -це система ідентифікації об'єктів контенту в цифровому середовищі. Система DOI забезпечує структуру для постійної ідентифікації, управління інтелектуальним контентом і метаданими, зв'язок користувачів із постачальниками контенту.Державне видавництво «Аграрна наука» НААН надає послуги присвоєння унікальних номерів -індексів DOI, які на сьогоднішній день стали однією з умов для включення видання у міжнародні наукометричні бази, такі як Scopus та Web of Science, і включення до Переліку наукових фахових видань України.Для участі у програмі CrossRef, будь ласка, зв'яжіться з видавництвом «Аграрна Aim. To evaluate potato breeding material for resistance to pathotypes of Synchytrium endobioticum (Schilbersky) Percival (1909) known to be present in Ukraine (pathotypes 1(D 1 ), 11, 13, 18 and 22); to identify resistant registered and potential varieties for the usage in the national wart disease eradication programs and to recommend these selected (potential and registered) potato varieties for the breeding program targeted on the development of multiple resistance against pathotypes of S. endobioticum present in Ukraine. Methods. Evaluation of the potato breeding material and registered potato varieties for the resistance against common path...
There were proposed the researches results for the wart potato on total area 468.6 ha in Zkarpattia, Ivano-Frankivsk and Chernivtsi regions. There were identified the infectious level on the present area. The highest infectious level is in the scope 56—110 zoosparingii on 1 g of soil in that wart potato sources in Zakarpattia Region. The infectious level reached 8—68 zoosparingii on 1 g of soil in Ivano-Frankivsk and Chernivtsi regions. The general area of wart potato sources was consisted 250.3 ha in Western region of Ukraine. There were identified 5 wart potato pathotypes with the help of Ukrainian differentiator varieties in Ukraine.
Goal. Harmonization system for determining potato wart causative agent as European union requirements for determination infectious load degree in Ukrainian sources. Methods. The field inspection was conducted as per the Standard PM3/59(3) and as per methodological recommendations for the samples extraction during the process of quarantine inspection and expertise. The winter zoosporangia causative agents conducted with calcium chloride and kaolin solution usage and flotation in sodium iodide solution. Results. The highest infectious load by zoosporangium disease pathogen was determined in observed 11 settlements in 4 regions of Ukraine from 39 to 43 zoosporangium on 1g of soil in Ivano-Frankivsk region, 49 in Lviv region, 65 — in Zakarpattia and Chernivtsi regions. The highest quantity of potato wart zoosporangium (to 65) was determined during the soils samples extraction as per the Protocole PM3/59(3) There were determined to 97.6% of potato wart zoosporangium in soil’s samples by flotation method in solutions with calcium chloride and kaolin. The researched soil’s sample weight decreased in twice or four times for the best pathogen identification in sources with high infectious load of disease agent. So kaolin is a white clay it paints zoosporangium in white color with next their masking That is why the causative agent viability determining treated by 3% hydrogen peroxide and painting by Coomassie Blue solution. The flotation method in sodium iodide with simultaneous determining pathogen viability, because sodium iodide painted them in intensive yellow colour. Conclusions. Soil samples extraction as per European Standard EPPO PM 3/59(3) was the most effective for determining potato wart zoosporangium. The technique for determining sodium iodide usage was the most favorable for extraction potato wart zoosporangium in disease sources. This technique allowed to determine and identify viability of pathogen winter zoosporangium. It was necessary to recommend the decrease the soil load in 2—4 times and for the next viability evaluation of winter zoosporangium with next painting by 0.5% solution of Coomassie Blue during the usage of solution calcium chloride and kaolin.
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