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Axis formation and related spatial patterning are initiated by symmetry breaking during development. A geometrically confined culture of human pluripotent stem cells (hPSCs) mimics symmetry breaking and cell patterning. Using this, polarized spinal cord organoids (pSCOs) with a self-organized dorsoventral (DV) organization are generated. The application of caudalization signals promoted regionalized cell differentiation along the radial axis and protrusion morphogenesis in confined hPSC colonies. These detached colonies grew into extended spinal cord-like organoids, which established self-ordered DV patterning along the long axis through the spontaneous expression of polarized DV patterning morphogens. The proportions of dorsal/ventral domains in the pSCOs can be controlled by the changes in the initial size of micropatterns, which altered the ratio of center-edge cells in 2D. In mature pSCOs, highly synchronized neural activity is separately detected in the dorsal and ventral side, indicating functional as well as structural patterning established in the organoids. This study provides a simple and precisely controllable method to generate spatially ordered organoids for the understanding of the biological principles of cell patterning and axis formation during neural development.

<p class="MsoNormal" style="text-align: left; margin: 0cm 0cm 0pt; layout-grid-mode: char;" align="left"><span class="text"><span style="font-family: ";Arial";,";sans-serif";; font-size: 9pt;">A new current-programming pixel circuit for active-matrix organic light-emitting diode (AM-OLED) displays, composed of four organic thin-film transistors (OTFTs) and one capacitor, has been designed, simulated and evaluated. The most critical issue in realizing AMOLED displays with OTFTs is the variation and aging of the driving-TFTs and degradation of OLEDs. This can cause image sticking or degradation of image quality. These problems require compensation methods for high-quality display applications, and pixel level approach is considered to be one of the most important factors for improving display image quality. Our design shows that the current OLED and OTFT technology can be implemented for AMOLED displays, compensating the degradation of OTFT device characteristics.</span></span><span style="font-family: ";Arial";,";sans-serif";; font-size: 9pt;"></span></p>

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