Context:Pseudomonas aeruginosa is a gram negative opportunistic bacteria causes several infections commonly colonize these devices and developing biofilms. Bacteria in biofilm can be up to 1,000 times more resistant to antibiotics than the same bacteria circulating in a planktonic state.Case Report:A total of 10 isolates of Pseudomonas aeruginosa were isolated from catheter associated urinary tract infections. While carbenicillin was the most effective antibiotic, all isolates developed multidrug resistant. Both crude and purified bacteriocin showed marked inhibition activity against planktonic and biofilm of the highly resistant isolate P. aeruginosa P7.Conclusion:Bacteriocin extracted from a locally isolated L. acidophilus has an anti P. aeruginosa biofilm activity also it can be used as a therapeutic agent after adequate in vivo experimentation.
Forty-two cyanoprokaryota strains were isolated from 10 diverse sites along Sharkia Governorate, Egypt (7 sites representing the fertile soils and 3 sites representing the new reclaimed soils). Eight genera and 11 species of N 2 -fixing cyanoprokaryota were identified based on available morphological distinction and physiological characterization. On the basis of the obtained results, we classified these isolates to 3 genera and 5 species isolated from fertile soils and 7 genera and 8 species isolated from new reclaimed soils. Nostoc spp strains were the most common filamentous heterocyst-forming cyanoprokaryota (22 strains) and two species of Nostoc including 11 strains of N. muscorum and 11 strains of N. calicicola. Anabaena includes 10 strains in three species (A. oryzae (6 strains), A. circinalis (2 strains) and A. oscillarioides (2 strains). Nodularia spumigena were represented in this work by two strains from fertile soils. In addition Calothrix clavata (2 strains), Synechococcus sp. (3 strains) and one strain of each Oscillatoria homogenea, Dermocarpa olivacea and Xenococcus kerneri were inhabited in the reclaimed soils. This indicates that Nostoc spp. and Anabaena spp. were highly distributed in fertile soils more than new reclaimed soils. The latter species were the most important cyanoprokaryota genera to fix atmospheric nitrogen in rice field.
A total of 20 isolates of lactic acid bacteria (LAB) were isolated anaerobically from different food sources (Domiati cheese; raw milk and mixed pickles). Three out of 20 isolates showed high inhibition of growth pathogenic bacteria by well diffusion assay method and therefore they were chosen for bacteriocin production and further studies. The selected isolates were identified based on morphological, biochemical and MALDI-TOF mass spectrometry. All of the tested species gave a score value between 2.116 to 2.165 (100%) were correctly identified by MALDI-TOF-MS to the genus and species levels. They were identified as L. brevis, L. plantarum and L. fermentum. The bacteriocin was purified by salt precipitation and gel chromatography methods. The molecular weight was determined by SDS-PAGE and amino acid composition was also analysed. The purified bacteriocin was characterized and found to be thermostable at temperatures up to 90°C for 30min, pH from 3 to 11 and its activity improved in the presence of Tween 80, SDS and EDTA. The contents of bacteriocin from the acidic amino acid residues aspartic + glutamic (asp+glu) were 0.74% for all tested samples. The contents of the basic amino acids argnine + lysine + hisitidine (arg + lys + his) were 0.23% for all the tested samples.
PPLICATION of organic amendments to soil is an important …….management strategy for enhancing soil fertility and microorganisms. A pot experiment was conducted under greenhouse conditions to investigate the effect of compost and compost tea(CT), mineral fertilization only complete (N 100) and half (N 50) recommended doses of nitrogen or combined with two kinds of compost tea (according to extract methods) molasses compost tea (CT1) and molasses compost tea+ ammonium sulphate (CT2) with three application ratios (v:v) (1:25, 1:50 and 1:75) on NPK uptake of leaf and root of radish (Raphanus sativus), soil nutrient status after harvest, and soil microbial populations. Application of CT1 showed that N uptake of radish leaf was about 30.1% higher than that of CT2. CT1 compost tea also increased P and K uptake by 12.6 and 51.5%, respectively, compared with CT2 application. At the end of experiment, soil N, P and K contents were greater in compost amended soil,whereas N uptake of radish leafand root showed only a significant positive correlation with soil N content in the CT2 + N treatments. The populations of the different microorganisms were greater in CT treatment, and increased with time as well. However, it was found that there was a significant negative correlation between leaf and root N uptake of radish and population of anaerobic N 2 fixing bacteria in soil after harvest.
A field experiment was carried out during two summer seasons of 2017and 2018 at Private Farm, at El-Salhyia District, Sharkia Governorate, Egypt, to study the effect of compost tea and some nutrient supplements on growth and yield of some potato cultivars (Bavana and Eliance) grown in loamy sand soil conditions. The results showed that, Bavana cultivar gave higher number of main stems/plant, shoots dry weight/plant and total chlorophyll in leaf tissues, whereas Eliance cultivar gave higher values of plant height, number of tubers/plant, average tuber weight, tuber yield/plant and total yield/ fed. and spraying potato plants with compost tea supplemented with NH4NO3( 0.5g/l) + molasses (0.5% v/v) led to increased number of leaves/plant, shoots dry weight, number of tubers/plant, average tuber weight, tuber yield/plant and total yield/ fed. In addition, spraying Bavana cultivar with compost tea supplemented with NH4NO3 + molasses or molasses or NH4NO3 gave the maximum values of number of main stems/plant, total chlorophyll, total carbohydrates and starch with no significant differences between them. On the other hand, spraying Eliance cultivar with compost tea supplemented with NH4NO3+molasses increased significantly plant height. Moreover, the interaction treatments between Bavana or Eliance plants and spraying compost tea supplemented with NH4NO3+ molasses or with molasses had significant effect on average tuber weight, tuber yield/plant, total yield/fed., DM% and P%. In addition, spraying Eliance cultivar with compost tea supplemented with NH4NO3 increased P and K contents in tubers, while, N and total protein in tubers were significantly enhanced due to spraying Bavana cultivar with compost tea supplemented with NH4NO3.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.