Objective To evaluate the sperm's chromatin quality in couples with spontaneous recurrent abortion. Methods Thirty couples with spontaneous recurrent abortion (case group) and 30 fertile couples (control group) referring to Zeinabieh Gynecology clinic of Shiraz were included. Semen samples were collected for each participant and were used for standard semen analysis and sperm nuclear maturity tests including Chromomycin A3 (CMA3), Aniline Blue (AB) staining and Acridine Orange (AO) test (by light microscopy).Result Patients in case group had significantly higher percentage of CMA3 (p < 0.001) and AB (p <0.001) positive spermatozoa compared to controls. However AO results did not differ significantly between groups (p= 0.656). Sperm morphology and progressive motility were negatively correlated with CMA3 (p=0.001 and p=0.043) and AB (p=0.015 and p=0.031) respectively. Conclusion Evaluation of the sperm's quality via CMA3 and AB staining could be considered as one of the complementary tests of semen analysis for assessment of male factor in couples with spontaneous recurrent abortion.

INTRODUCTION:We investigated the antianxiety and sedative effects of the essential oil of Ducrosia anethifolia. Boiss. (Apiaceae).METHODS:We used elevated plus maze, spontaneous motor activity and ketamine‐induced sleep tests in mice. In addition, the essential oil was analyzed by GC/MS. Twenty compounds were identified, and n‐decanal (70.1%) and alpha‐pinene (12.4%) constituted the major components.RESULTS:In elevated plus maze, Ducrosia anethifolia essential oil at doses of 25–200 mg/kg increased the percentage of open arm time and entries. Unlike diazepam, ducrosia anethifolia essential oil could not suppress spontaneous motor activity and did not alter ketamine‐induced sleep parameters. These results are indicative of antianxiety effect of Ducrosia anethifolia essential oil without sedative effect.

Cell therapy consisting of intrasphincteric and submucosal injections of autologous TNCs along with platelets in SUI patients is a feasible and safe procedure. The results point out those subjects cured or with marked improvement after 6 months F/U.

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