We have found that transition of actively dividing Mycobacterium smegmatis cells into the dormant "nonculturable" state is accompanied by increase in the protein/lipid ratio and disappearance of one of the main lipid components of the mycobacterial cells, trehalose monomycolate. In this case, oleic acid is accumulated in the culture medium due to its secretion by the mycobacterial cells. Addition of lipids of different classes to "nonculturable" M. smegmatis cells induces their resuscitation. The lipid reactivating effect is evidently caused by the presence of fatty acids in their composition, because free fatty acids also exhibited reactivation effect. Oleic acid in concentration of 0.05-3 μg/ml exhibited maximal effect, and that allows us to draw a conclusion concerning its signal role in the transition of dormant cells into active state.
Hydro distilled essential oils in air-dry samples of aerial parts of Solidago canadensis L., (Asteraceae) from eight local invasive populations were investigated by GC-MS analysis. A comparative study on quantity and composition of the essential oils obtained from plants, growing in different ecological and climatic conditions, ontogenesis phase and different plant organs was carried out The major compounds detected in oil samples of S. canadensis were α-pinene (1.3 -61.27%), limonene (0.5 -22.5%), bornyl acetat (3.4 -29.8%) and germacrene D (1.8 -39.2%). Samples from inflorescences contained the maximal percentage of monoterpene hydrocarbons, while the leaves' samples showed the maximal cumulative percentage of sesquiterpene and monoterpene hydrocarbons. Data obtained from our studies confirm the availability of alien invasive species Solidago canadensis for medicine and many other purposes. The variability of the qualitative and quantitative composition of essential oils in different geographical locations will allow futher selection of form containing the maximum amount of active substances.
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