Summary. High titred monospecific antibodies are obtained in rabbits injecting as little as 25 μg pure antigen per kg body weight. For polyspecific antibodies the dose is increased 10 times. The immunization schedule is described in detail. A procedure is given for the isolation of the immunoglobulins from antiserum by salting out and ion exchange chromatography. Finally antibody titres as measured by 4 different titration methods are compared using anti‐human IgG from rabbits as a model.

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Many antibody preparations exhibit proteolytic activity due to the presence of plasmin. In crossed immunoelectrophoresis at pH 8.6 this enzyme can degrade certain proteins during electrophoresis in the antibody-containing gel, resulting in artefacts in the form of extra precipitation arcs of congruent shape. The degradation behaviour of spectrin, a major protein of human erythrocyte membranes, was investigated. The artefact could be completely abolished by the addition of protease inhibitors, e.g. aprotinin and soya bean trypsin inhibitor, to the antibody preparations. 0. J. Bjerrum, M . D., The Protein Laboratory, Sigurdsgade 34, DK-2200 COpenhagen, Denmark SUPPI. 2, 81-88, 1975.

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