Single Radial Immunodiffusion

Ingild, A.

doi:10.1111/j.1365-3083.1983.tb03999.x

Cited by 33 publications

(8 citation statements)

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“…The amounts of grass group 1 and 5 allergens in the extracts of the 10 species were quantified by SRID [8]. Purified nPhl p 1 and nPhl p 5 were used to construct linear standard curves, and the concentration of group 1 and 5 allergens was determined from the standard curves and expressed as nPhl p 1 or nPhl p 5 equivalent amounts (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Quantification of Group 1 and 5 allergens. The amounts of grass group 1 and 5 allergens in 10 different single‐species grass pollen extracts were determined by SRID [8]. The pollen extracts from the individual grass species were applied in different concentrations selected to be within the range of the amounts of either nPhl p 1 or nPhl p 5 used to obtain a standard curve (determined from pilot studies).…”

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confidence: 99%

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Phleum pratense alone is sufficient for allergen‐specific immunotherapy against allergy to Pooideae grass pollens

Hejl

Würtzen

Kleine-Tebbe³

et al. 2009

Clin Experimental Allergy

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Phleum pratense alone is sufficient for allergen‐specific immunotherapy against allergy to Pooideae grass pollens

Hejl

Würtzen

Kleine-Tebbe³

et al. 2009

Clin Experimental Allergy

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“…Serum IgM concentrations were determined by single radial immunodiffusion in 0.9% (w/v) agarose gels containing 1 pliml of a rabbit anti-human IgM antibody (DAKO, Copenhagen) (8). Pooled human serum with a known IgM concentration was used as the standard.…”

Section: Determinution Of Total Igmmentioning

confidence: 99%

Serological and clinical findings in patients with serological evidence of reactivated Epstein-Barr virus infection

Obel

Høier‐Madsen

Kangro

1996

Apmis

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Obel, N., Herier-Madsen, M. & Kangro, H. Serological and clinical findings in patients with serological evidence of reactivated Epstein-Barr virus infection. APMIS 104: 424428, 1996.IgM directed against Epstein-Barr virus (EBV) early antigen (IgM-EA) has been established as an early marker of EBV infection and IgG directed against Epstein-Barr virus nuclear antigen I (IgG-EBNA-1) as a late marker. Simultaneous seropositivity to IgM-EA and IgG-EBNA has therefore been proposed as indicating reactivation of latent EBV infection. We have studied 191 patients with serological evidence of reactivated EBV infection with regard to clinical presentation, antibodies directed against EBV viral capsid antigen (IgM-VCA, IgG-VCA), cytomegalovirus (IgM-CMV). human herpesvirus 6 (IgM-HHV-6), IgM rheumatoid factor (IgM-RF), anti-nuclear or anti-cytoplasmic antibodies (ANA/ACA), and total IgM. The clinical manifestations varied considerably. but a diagnosis was established in 121 of the patients. The diversity of the clinical diagnosis probably reflects common reasons for requesting an EBV serological test rather than clinical manifestations of reactivated EBV infection. Only 5.8% of the patients with "serological EBV reactivation" gave a positive result for IgM-VCA. We conclude that "serological EBV reactivation" does not represent an entity relating to clinical manifestations, but probably reflects a non-specific activation of the immune system.

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“…The content of IgA and IgM was measured by radial immunodiffusion [39] and by in-house capture ELISAs (with rabbit anti-IgM or anti-IgA as capture antibodies and the corresponding peroxidase-conjugated antibodies as detecting antibodies, all from DAKO, Copenhagen) using a calibrated reference serum (X908) for calibration.…”

Section: Methodsmentioning

confidence: 99%

“…IgG subclasses were quantified by radial immunodiffusion as described [39], using a WHO reference serum (67/97) for calibration.…”

Section: Methodsmentioning

confidence: 99%

Development, Manufacturing and Characterization of a Highly Purified, Liquid Immunoglobulin G Preparation from Human Plasma

Laursen

Blou

Sullivan

et al. 2014

Transfus Med Hemother

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Background: The use of plasma-derived immunoglobulin G (IgG) is increasing, and the number of diseases, including immunodeficiencies, neurological diseases and autoimmune conditions, treated with intravenous IgG (IVIG) is expanding. Consequently, there is a great need for high-yield production processes for plasma-derived IgG. The aim of this work was to develop a high-yield process leading to a highly purified, liquid, ready-to-use IgG for intravenous use. Methods: Plasma from healthy, voluntary, non-remunerated donors was fractionated by ethanol precipitation. IgG was extracted from fraction II + III using a phosphate/acetate buffer, pH 4, and purified by chromatography. Results: Precipitation with 6% polyethylene glycol at pH 7 removed high molecular-weight contaminating proteins, aggregates and contaminating viruses. Ion exchange chromatography at pH 5.7 on serially connected anion and cation exchange columns allowed for elution of IgG from the cation exchange column in good yield and high purity. Further safety was achieved by solvent/detergent treatment and repeated ion exchange chromatography. The product consisted of essentially only IgG monomers and dimers, and had a high purity with very low levels of IgM and IgA. Conclusion: A process providing highly purified IVIG in good yield was developed.

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Single Radial Immunodiffusion

Cited by 33 publications

References 0 publications

Phleum pratense alone is sufficient for allergen‐specific immunotherapy against allergy to Pooideae grass pollens

Phleum pratense alone is sufficient for allergen‐specific immunotherapy against allergy to Pooideae grass pollens

Serological and clinical findings in patients with serological evidence of reactivated Epstein-Barr virus infection

Development, Manufacturing and Characterization of a Highly Purified, Liquid Immunoglobulin G Preparation from Human Plasma

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