The effects of glutaraldehyde, formaldehyde, or osmium tetroxide fixation on the number of labeled Con A surface receptors on mouse peritoneal macrophages were compared. Gold-labeled Con A receptors were found to be isolatedly arranged and evenly distributed on cell surfaces independent of the fixative used. Only cells preincubated with Con A and subsequently fixed by osmium tetroxide showed arrangement of labeled receptors in clusters. Significant differences were found in the number of Con A receptors per cell depending on the fixative used. The fluorescence intensity of FITC-Con A staining was detected spectrophotometrically, the characteristic X-rays of gold-labeled Con A receptors were determined by means of electron beam-induced X-ray microanalysis. The experimental results obtained both at light and electron microscopic level pointed to formaldehyde being the best fixative also for this purpose.
Einyeynngen a m I d . 5 . 1979) Es wurden c'n. 160 Hefestamrne aus verschiedenen Familien, Gattungen untl Arten dcr Asconiyceten, Deuteromyceten nnd Basidiomyceten bei konstanter Zellzahl iind Lektinkonzentration auf ihre Agglutinierbarkeit durch Lektine getestet (Concanavalin A, Lens culinnrzs-Hamagglutiiiin, Weizenkeimlingsagglutinin, Dolichos biflorus-Agglutinin, Erdn u6-Hhmagglutinin, Kicinva comr/~unis-Hdmagglutinin). Dsbci ergaben sich gwppenspezifische hggluti~~ationsmuster. Ein Stamm der Art Hhodosporidiurn toruloides kann durch Welzenkeirnlingsagglutinin zur Agglutination gebracht werden.Einige Hefestamme der Gsttung Rhodotorula und Rhodosporidium lassen sich durch die untersuchten Lektine niclit agglutinieren. Sie besitzen offenbar gegenilber den anderen gepruften Hefcn deutlich nbweichcnde Zellw andstrukturen.
The investigation presented was aimed at studying microecological phenomena during and after microbial penetration of the gap between restoration and tooth substance and to verify our theory on 'microbiosphere in the marginal gap after filling'. Eighty-two teeth filled with different restorative materials were investigated by means of a combination of methods including light microscopy, transmission electron microscopy and scanning electron microscopy. During bacterial invasion into the space between a restoration and the tooth phenomena of microbial viability such as bacterial adherence, interaction, proliferation, penetration, and metabolism were observed. The relevance of these phenomena for microbial penetration along the restoration/tooth interface and their relationship to the onset of secondary carious lesions is discussed.
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