“…Different combinations of high speed centrifugation, ammonium sulphate precipitation, chromatography on hydroxylapatite (O'hEocha, 1962;Roth et al, 1989;Sidler et al, 1989), hydrophobic chromatography (Leibo and Jones, 1964;Schoenleber et al, 1983;Roth et al, 1989) ion -exchange chromatography (Thepenier et al, 1987) gel filtration (Gantt, 1969;Schoenleber et al, 1983;Jahn et al, 1984) etc. were used for isolation and purification of B-PE from the crude extract.…”